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The map label for this gene is spt [H]

Identifier: 126696278

GI number: 126696278

Start: 808548

End: 809732

Strand: Direct

Name: spt [H]

Synonym: P9301_09401

Alternate gene names: 126696278

Gene position: 808548-809732 (Clockwise)

Preceding gene: 126696274

Following gene: 126696280

Centisome position: 49.25

GC content: 34.68

Gene sequence:

>1185_bases
TTGACGGAAGCAAAAATTATTTCGGCTTTAAATGAAGAGAATTTTTCTCATTTCTCAAAGACTTATGTTCCCTCTAGACT
TTTATTAGGTCCTGGTCCTTCAAACGCACATCCAGAAGTCTTAAGCGCTCTTTCTTTGAATCCTATTGGCCACTTAGATG
AAGCATATATTTCATTGATGTCTGACGTTCAACAACTTCTTAGATATACCTGGCAATGTAATAATCGTCTGACTCTTCCA
ATGAGTGGCACTGGAAGTGCAGCTATGGAAGCCTCAATAGCTAATTTTATAGAGGAGGGAGAAAAAATTCTTATCGCTAA
AAAAGGATATTTTGGAGACAGACTTGTTGATATGGCAACAAGATATAAAGCAGATGTATCTGTTATGGAAAAACCTTGGG
GTGAATCCTTTTCTTATGAAGAAATCAAGTATGAAATAGAAACTAAAAAACCAGCAATTTTTGCTATCGTCCATGCTGAA
ACATCAAGTGGGGTTTTACAACCTCTTAATGGAATTGGTGAAGTATGTAGAAAAAATAACTGCTTGTTTTTAGTTGACGC
AGTTACATCCCTCGGCGCTCTTGAACTATTGATAGATGAATGGAAAATAGATTTAGCATATAGTTGTAGCCAGAAAGGAT
TAAGCTGTCCGCCTGGACTAAGTCCTTTTACAATGAATAAGAGAGCTGAAGATAAACTAAGTTCAAGAAAAACAAAAGTC
CCAAACTGGTATTTAGATTTATCTCTTTTAAATAAATATTGGGGTTCTGATCGCGTTTATCATCATACGGCCCCTGTAAA
TATGAATTTTGCTATTCGAGAAGGTTTACGATTAATTGCAAATGGTGGTTTAGATAATGTTTGGTATAGACATAATACTA
ATGCAAAGAAACTTTGGAAAGGATTAGAAAGTCTAGGCATGGAATTACATGTATCAGAGGATTATAGATTGCCAACTCTT
ACAACAGTTAAGATCCCACCTGCAGTAGATGGAGATGGTTTTAGAAATCATCTTTTAAGAAAATTTGGAATAGAAATAGG
AAATGGACTTGGAGAATTGTCTGGTAAGGTTTGGCGCATAGGATTAATGGGCTTTAACTCAAGTGAGGAGAATGTAGATA
GATTATTAAACCTATTTGATACTGAATTAAAAAAATATTCTATTTTTGAGTCCTCAACTTTTTAA

Upstream 100 bases:

>100_bases
TAATTGTTTACTTAAATATTTAAATTGAATTAATTTCTTTAAATAAATATCGCTTATATGGAGATATTAGTTTAATTTAA
AAGTAATTGAATGTAATGCT

Downstream 100 bases:

>100_bases
ACCATAATTGTAAATTTTTACAGCATTCGTCTTCTAAAATACCTCCTACGATTTCCATTTTGTGATGAGAACTTTTATGC
TTCGATAAATCAATTGAGCC

Product: serine:pyruvate/alanine:glyoxylate aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTEAKIISALNEENFSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLMSDVQQLLRYTWQCNNRLTLP
MSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMATRYKADVSVMEKPWGESFSYEEIKYEIETKKPAIFAIVHAE
TSSGVLQPLNGIGEVCRKNNCLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRKTKV
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANGGLDNVWYRHNTNAKKLWKGLESLGMELHVSEDYRLPTL
TTVKIPPAVDGDGFRNHLLRKFGIEIGNGLGELSGKVWRIGLMGFNSSEENVDRLLNLFDTELKKYSIFESSTF

Sequences:

>Translated_394_residues
MTEAKIISALNEENFSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLMSDVQQLLRYTWQCNNRLTLP
MSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMATRYKADVSVMEKPWGESFSYEEIKYEIETKKPAIFAIVHAE
TSSGVLQPLNGIGEVCRKNNCLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRKTKV
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANGGLDNVWYRHNTNAKKLWKGLESLGMELHVSEDYRLPTL
TTVKIPPAVDGDGFRNHLLRKFGIEIGNGLGELSGKVWRIGLMGFNSSEENVDRLLNLFDTELKKYSIFESSTF
>Mature_393_residues
TEAKIISALNEENFSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLMSDVQQLLRYTWQCNNRLTLPM
SGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMATRYKADVSVMEKPWGESFSYEEIKYEIETKKPAIFAIVHAET
SSGVLQPLNGIGEVCRKNNCLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRKTKVP
NWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANGGLDNVWYRHNTNAKKLWKGLESLGMELHVSEDYRLPTLT
TVKIPPAVDGDGFRNHLLRKFGIEIGNGLGELSGKVWRIGLMGFNSSEENVDRLLNLFDTELKKYSIFESSTF

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=366, Percent_Identity=41.5300546448087, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17536281, Length=369, Percent_Identity=38.7533875338753, Blast_Score=287, Evalue=8e-78,
Organism=Saccharomyces cerevisiae, GI6321079, Length=375, Percent_Identity=26.9333333333333, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI17530823, Length=368, Percent_Identity=40.7608695652174, Blast_Score=307, Evalue=9e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 44125; Mature: 43994

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAKIISALNEENFSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLM
CCHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SDVQQLLRYTWQCNNRLTLPMSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMAT
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RYKADVSVMEKPWGESFSYEEIKYEIETKKPAIFAIVHAETSSGVLQPLNGIGEVCRKNN
HHHHCHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCC
CLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRKTKV
CEEEEECHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANGGLDNVWYRHNTNAKKLWK
CCEEEEHHHHHHHCCCCCEEEECCCCCCCHHHHHCHHEEECCCCCCCEEEECCCHHHHHH
GLESLGMELHVSEDYRLPTLTTVKIPPAVDGDGFRNHLLRKFGIEIGNGLGELSGKVWRI
HHHHCCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCHHHCCCHHHCCCCEEEE
GLMGFNSSEENVDRLLNLFDTELKKYSIFESSTF
EEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TEAKIISALNEENFSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLM
CHHHHHHHHCHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SDVQQLLRYTWQCNNRLTLPMSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMAT
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RYKADVSVMEKPWGESFSYEEIKYEIETKKPAIFAIVHAETSSGVLQPLNGIGEVCRKNN
HHHHCHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCC
CLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEDKLSSRKTKV
CEEEEECHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANGGLDNVWYRHNTNAKKLWK
CCEEEEHHHHHHHCCCCCEEEECCCCCCCHHHHHCHHEEECCCCCCCEEEECCCHHHHHH
GLESLGMELHVSEDYRLPTLTTVKIPPAVDGDGFRNHLLRKFGIEIGNGLGELSGKVWRI
HHHHCCCEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCHHHCCCHHHCCCCEEEE
GLMGFNSSEENVDRLLNLFDTELKKYSIFESSTF
EEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]