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The map label for this gene is gatA

Identifier: 126696250

GI number: 126696250

Start: 782885

End: 784333

Strand: Direct

Name: gatA

Synonym: P9301_09121

Alternate gene names: 126696250

Gene position: 782885-784333 (Clockwise)

Preceding gene: 126696249

Following gene: 126696251

Centisome position: 47.68

GC content: 36.37

Gene sequence:

>1449_bases
ATGAATTTTAATTCTTTAAGAAAAGAAATTTTCAGCAATAATGCTTCTGTTAAGGAACTAGTCAATGATATCTTTACCAA
TATCGATCATAAAGACCCTGAAATTAACTCATATATTTGTACTACAAAAGATAATGCCATCGCGCAAGCAGAGAACATTG
ATAAATTAATTCAATTTAAAGAAAAACTGCCCCCTCTCGCTGGGATGCCGATAGCAATAAAGGATAATATTTGCACTAAA
GGAGTTGCAACAACCTGTGCAAGTAAAATGCTCAAAAACTTTGTTGCTCCTTATGAATCTACAGCTTCAAGTAAATTATG
GTCTTCAGGAGGAATTTGTTTAGGAAAAACAAATTTAGACGAATTTGCAATGGGTAGTTCAACGGAAACCTCTCTATTTG
GTGTTACTTCAAATCCTTGGGATATTAATAGAGTGCCAGGAGGAAGTTCAGGCGGTAGTGCTGCTTCAGTTGCTGCTGGA
TTTTGCGCGGCTGCTATAGGTTCTGACACAGGAGGATCAATAAGACAGCCAGCTTCATTTTGTGGGGTTGTAGGTCTTAA
ACCTACTTATGGCAGAGTTAGTAGATGGGGACTGGTAGCATTCGCTAGTTCTCTTGATCAAATTGGACCAATCACAAATA
CTGTCTCAGATGCTGCTGAAATTCTTTATTCAATTTCTGGTAAAGATCCCTTTGACTCTACATGTCTTGATAAGCCAGTA
CCAAATTACTTGACTCACTTAAATAAATCTATAAAAGGTTTAAAAATTGGAATCATTAAAGAATGTTTTGAGCACCCAGG
TCTTAATCCAGAAGTTAAGGAATCTGTTCTTTCTGGAGTTGATAGATTCCAAGCTTTGGGGGCTGAAATCATCGAAGTTG
AATGTCCGAGATTTAACGATGGAATTGCGACCTATTATGTCATTGCACCATCTGAAGCCTCCGCAAATTTAGCTAGATAT
GATGGAGTCAAATATGGCTACAGATCGAATGATGGCTCAAATCTTATAGATATGACTTCTAAAAGTAGAGCTGAAGGTTT
TGGAGATGAGGTACAAAGAAGAATTTTGATAGGTACTTATGCTTTGTCAGCTGGATACAGTGATGCCTATTACAAGAAGG
CACAAAAAGTTAGGACACTCATTAGAAAAGATTTTGATAATGCTTTTAATAAAGTAGATGTTTTATTAACTCCAACTTGC
CCAACTACCGCTTTTTTGAAGGGAGATTTTGTCAATGATCCACTTTCAATGTATTTATCTGATCTATTAACTGTTCCTGC
TAATTTAGCTGGCCTCCCAGCAATCAGTATTCCTTGTGGTTTTGATACGAAGGGATTACCTATTGGAATGCAATTAATAG
GCAATGTATTAGAAGAAGATAGAATATTGAATGCCGCAAATATCTTTGAAATTGATGCTCAGGTAATTAAGAACAGACCT
TCATTTTAA

Upstream 100 bases:

>100_bases
TGATGAAGGATCTAAAAATATTAAACACAGTTTAATACGTTGTAAAAAAGAAGAACCATTAACTGTTTGATAACTTTGAT
TTTAAGAAATAAATTAAGAA

Downstream 100 bases:

>100_bases
ATTTGTATTAATAATTAATTTGTAATCACAAAGTATAGATCTTTTAGAAATTTTCTCTATCTTATATATATAAATTGATT
GAATATGGCTTTCGTACCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MNFNSLRKEIFSNNASVKELVNDIFTNIDHKDPEINSYICTTKDNAIAQAENIDKLIQFKEKLPPLAGMPIAIKDNICTK
GVATTCASKMLKNFVAPYESTASSKLWSSGGICLGKTNLDEFAMGSSTETSLFGVTSNPWDINRVPGGSSGGSAASVAAG
FCAAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPFDSTCLDKPV
PNYLTHLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGYRSNDGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRKDFDNAFNKVDVLLTPTC
PTTAFLKGDFVNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGMQLIGNVLEEDRILNAANIFEIDAQVIKNRP
SF

Sequences:

>Translated_482_residues
MNFNSLRKEIFSNNASVKELVNDIFTNIDHKDPEINSYICTTKDNAIAQAENIDKLIQFKEKLPPLAGMPIAIKDNICTK
GVATTCASKMLKNFVAPYESTASSKLWSSGGICLGKTNLDEFAMGSSTETSLFGVTSNPWDINRVPGGSSGGSAASVAAG
FCAAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPFDSTCLDKPV
PNYLTHLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGYRSNDGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRKDFDNAFNKVDVLLTPTC
PTTAFLKGDFVNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGMQLIGNVLEEDRILNAANIFEIDAQVIKNRP
SF
>Mature_482_residues
MNFNSLRKEIFSNNASVKELVNDIFTNIDHKDPEINSYICTTKDNAIAQAENIDKLIQFKEKLPPLAGMPIAIKDNICTK
GVATTCASKMLKNFVAPYESTASSKLWSSGGICLGKTNLDEFAMGSSTETSLFGVTSNPWDINRVPGGSSGGSAASVAAG
FCAAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPFDSTCLDKPV
PNYLTHLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGYRSNDGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRKDFDNAFNKVDVLLTPTC
PTTAFLKGDFVNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGMQLIGNVLEEDRILNAANIFEIDAQVIKNRP
SF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=468, Percent_Identity=41.4529914529915, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI195972892, Length=490, Percent_Identity=24.2857142857143, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI166795287, Length=231, Percent_Identity=29.4372294372294, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=448, Percent_Identity=37.9464285714286, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=470, Percent_Identity=26.5957446808511, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17538252, Length=316, Percent_Identity=28.7974683544304, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=281, Percent_Identity=30.9608540925267, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17556264, Length=241, Percent_Identity=31.9502074688797, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17556278, Length=313, Percent_Identity=30.9904153354633, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=277, Percent_Identity=30.6859205776173, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=321, Percent_Identity=24.6105919003115, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=422, Percent_Identity=35.781990521327, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6319685, Length=448, Percent_Identity=32.3660714285714, Blast_Score=123, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6320448, Length=133, Percent_Identity=39.0977443609023, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648113, Length=457, Percent_Identity=43.7636761487965, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24652985, Length=481, Percent_Identity=25.1559251559252, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922090, Length=481, Percent_Identity=25.1559251559252, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652981, Length=481, Percent_Identity=25.1559251559252, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652983, Length=481, Percent_Identity=25.1559251559252, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24644968, Length=483, Percent_Identity=26.0869565217391, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI161078093, Length=485, Percent_Identity=28.0412371134021, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45550774, Length=490, Percent_Identity=24.2857142857143, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648435, Length=490, Percent_Identity=24.2857142857143, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648437, Length=490, Percent_Identity=24.2857142857143, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24648441, Length=432, Percent_Identity=24.537037037037, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648439, Length=432, Percent_Identity=24.537037037037, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356731, Length=465, Percent_Identity=23.6559139784946, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PROM0 (A3PCR0)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091136.1
- ProteinModelPortal:   A3PCR0
- SMR:   A3PCR0
- STRING:   A3PCR0
- GeneID:   4912320
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_09121
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   PMAR167546:P9301ORF_0929-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51812; Mature: 51812

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 74-74 ACT_SITE 149-149 ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFNSLRKEIFSNNASVKELVNDIFTNIDHKDPEINSYICTTKDNAIAQAENIDKLIQFK
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
EKLPPLAGMPIAIKDNICTKGVATTCASKMLKNFVAPYESTASSKLWSSGGICLGKTNLD
HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCH
EFAMGSSTETSLFGVTSNPWDINRVPGGSSGGSAASVAAGFCAAAIGSDTGGSIRQPASF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPFDSTCLDKPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PNYLTHLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFND
HHHHHHHHHHHCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRSNDGSNLIDMTSKSRAEGFGDEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHEEEH
ALSAGYSDAYYKKAQKVRTLIRKDFDNAFNKVDVLLTPTCPTTAFLKGDFVNDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDTKGLPIGMQLIGNVLEEDRILNAANIFEIDAQVIKNRP
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SF
CC
>Mature Secondary Structure
MNFNSLRKEIFSNNASVKELVNDIFTNIDHKDPEINSYICTTKDNAIAQAENIDKLIQFK
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
EKLPPLAGMPIAIKDNICTKGVATTCASKMLKNFVAPYESTASSKLWSSGGICLGKTNLD
HHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCH
EFAMGSSTETSLFGVTSNPWDINRVPGGSSGGSAASVAAGFCAAAIGSDTGGSIRQPASF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPFDSTCLDKPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PNYLTHLNKSIKGLKIGIIKECFEHPGLNPEVKESVLSGVDRFQALGAEIIEVECPRFND
HHHHHHHHHHHCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGYRSNDGSNLIDMTSKSRAEGFGDEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCHHHHCCCCHHHHHHHHEEEH
ALSAGYSDAYYKKAQKVRTLIRKDFDNAFNKVDVLLTPTCPTTAFLKGDFVNDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDTKGLPIGMQLIGNVLEEDRILNAANIFEIDAQVIKNRP
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
SF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA