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The map label for this gene is glyS [H]

Identifier: 126696157

GI number: 126696157

Start: 708223

End: 710385

Strand: Direct

Name: glyS [H]

Synonym: P9301_08191

Alternate gene names: 126696157

Gene position: 708223-710385 (Clockwise)

Preceding gene: 126696155

Following gene: 126696159

Centisome position: 43.13

GC content: 29.17

Gene sequence:

>2163_bases
TTGTCTAAATATTTACTTGAGATAGGAACAGAAGAGTTGCCCGCAAAATTTTCTTATTCTGTTCTAGAGCAATTTAAATC
TCTAATAGAATTTGAATTGGATAAAAAGTTAATCAAATTCGAACATATAGTTGTTACCTCCACACCAAGGAGGATAGTTC
TACTTCTCGAAGGTTTAGTTGATTATGCAGAAGATAAAATAATAGAAAGGAAAGGGCCTAAAGCAAATTCAGCTTATTTA
AATGGATGTCCTACTAATGCTGCTTTGGGATTTGCTAATAGCTTAGATATAAATGTTGGTGAGCTAGAAATAAAAAATAC
GGAAAAGGGTGATTTTGTATTTGGAAAGAAAATTGAGAAAGGACTATCAACAAAAATTTCTTTGTCTTCGATTATCCCAA
AATTAGTAAAGAGTCTTCAAGGCGCTCGATTTATGAAATGGGGGGCCGGGAACATGAAATTTTCAAGACCAATTAGGTGG
ATTGCCTCTATTTATAATGATGAAATTCTTGATTTTGAATTTGATGAATGTGATCCAAAAATCAAAATAAGTAATAAAAC
AAAAAGTCATAGGCTAATCAATGAAGTTTTAGAAGTTCAGAATCCTGATGAATTTTTTGAATTATTGAAACGAAATAGAG
TAATAGCTATTCGAAAAGAAAGAAAAGAAAAAATTGAAAGTTTAATAAATCAGGCATCTAAATCTTTAAATCTGGAACCT
GACCTTTCAGAAGGATTACTAAATGAACTTACTGATTTAGTTGAATGGCCAGACTTAATTATTGGCAAATTTAGTAATGA
ATTTCTTGATCTTCCTGTTGAAGTCCTCTCAACAGTCATGAAAATTCATCAGAGATACGTTCCTCTTTTATTGAAAAATG
AAAGTTTTTCTAAACTAGATTTAAGCTCTGAAAAAAATATTAGTACAACTTTCTGCGTTATTTCAAATGGTCTTGAAGAA
TCAAATAATAATATTGCCAAAGGTAATGAGAAAGTATTAAGGGCAAGGTTTTCAGATGCAAAGTTTTTCGTAGAAAGTGA
CAAAAAAGTTGCTTCAATCGAAAGAAATGAAAAGCTTAAATCTGTTTCATATTTGAAAGGACTTGGAAATATATTTCAGA
GGGTAGAAAGGATAGAGGAAGTTACTAAAAAAATTCTTAAATTTTTAAATGATAAGTCTTTAGAAGAAAAAAAAATAATA
GAAGCTGCTAAATACTGCAAAAACGATTTATGTAGTGAAATTGTTTTCGAATTCCCTGAGCTGCAAGGAATAATGGGTGC
TAAATATCTTAAATATGAGGGATTTAGTGAGGATGTTTGCTTGGCTGTCGCTGAACATTATTTACCTTCTTTTTATAAAG
ATGCTTTGCCCTCCACAAAATATGGTGCAATAGTTTCCATAGCAGATAAGGTCGAAACTTTAATAAGTATATTTATTTCT
GGTAAACGTCCCAGTGGATCATCTGATCCTTATGCTTTAAGAAGAAATTTGAATGGAGTTATTAAAATAATTTGGGATTA
CGAATTTGATTTGCCTTTAGATAAATTATTTAACGAACTTATTGATTTTTGGAAAATCGCATTTCCGAATTTAAACTTCT
CAAGAGAAAAAGTTTTTAATGATTTAAATGAATTTTTAGTTCAAAGAATTGTTAGTCATCTAGAAGAAATATCACTAAGT
AAAGAATTAATAAGGGCCGTTTGCTCTTCTGATGAATTATCTAAAAAAAGAGTATTGAATATTGTTGATCTTAAAAATAG
GATTAAATCTATTATGGATTTTAATGAAAAGGAAAATTTTGTTGAAATCCAGAAGGTAATTACTAGGGCAAGCAAATTAG
CTAATAATAGTGATCTTTCAACAGACGTTCTCGCTACAAGAGATTATGTAAACACAAAACTTTTTGAAAAGGATTGCGAA
TTGAAAGTTTTTGAATTTATTGAAGAATTAGAAAAACTTTTTTCAGAAGATTATTGCAATTATTTGGAACTTCTAAATTT
GTTTGAGATTAATGTAAATACTATCGAGGATTTATTTGATAATGAAAAAGGAGTCCTAATAATGTCAGAGGATTTAAAAA
TAAGAAATAATAGACTCAATTTGTTGAGCTTAATTAGAAATTATTCTCTAAAAATAGCCGACTTTACACTTTTGAACTCT
TAA

Upstream 100 bases:

>100_bases
CAACCTTGATCAAAGGACCAAAACATATCTCTTTTTATACATTAGCGAAAAGCTTATTTAACTGGTAGTAAATTATAGTA
AGAATAGTCATATTTTTTTC

Downstream 100 bases:

>100_bases
CTTTAATGCCTCCCTTTATCGAATAATTTTCTAGCATTTTTTCTACTTCTTTATTTTCCCTGACGGCACTATCAACGGGT
TTATATTTTAAAGGCGCTAA

Product: glycyl-tRNA synthetase beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MSKYLLEIGTEELPAKFSYSVLEQFKSLIEFELDKKLIKFEHIVVTSTPRRIVLLLEGLVDYAEDKIIERKGPKANSAYL
NGCPTNAALGFANSLDINVGELEIKNTEKGDFVFGKKIEKGLSTKISLSSIIPKLVKSLQGARFMKWGAGNMKFSRPIRW
IASIYNDEILDFEFDECDPKIKISNKTKSHRLINEVLEVQNPDEFFELLKRNRVIAIRKERKEKIESLINQASKSLNLEP
DLSEGLLNELTDLVEWPDLIIGKFSNEFLDLPVEVLSTVMKIHQRYVPLLLKNESFSKLDLSSEKNISTTFCVISNGLEE
SNNNIAKGNEKVLRARFSDAKFFVESDKKVASIERNEKLKSVSYLKGLGNIFQRVERIEEVTKKILKFLNDKSLEEKKII
EAAKYCKNDLCSEIVFEFPELQGIMGAKYLKYEGFSEDVCLAVAEHYLPSFYKDALPSTKYGAIVSIADKVETLISIFIS
GKRPSGSSDPYALRRNLNGVIKIIWDYEFDLPLDKLFNELIDFWKIAFPNLNFSREKVFNDLNEFLVQRIVSHLEEISLS
KELIRAVCSSDELSKKRVLNIVDLKNRIKSIMDFNEKENFVEIQKVITRASKLANNSDLSTDVLATRDYVNTKLFEKDCE
LKVFEFIEELEKLFSEDYCNYLELLNLFEINVNTIEDLFDNEKGVLIMSEDLKIRNNRLNLLSLIRNYSLKIADFTLLNS

Sequences:

>Translated_720_residues
MSKYLLEIGTEELPAKFSYSVLEQFKSLIEFELDKKLIKFEHIVVTSTPRRIVLLLEGLVDYAEDKIIERKGPKANSAYL
NGCPTNAALGFANSLDINVGELEIKNTEKGDFVFGKKIEKGLSTKISLSSIIPKLVKSLQGARFMKWGAGNMKFSRPIRW
IASIYNDEILDFEFDECDPKIKISNKTKSHRLINEVLEVQNPDEFFELLKRNRVIAIRKERKEKIESLINQASKSLNLEP
DLSEGLLNELTDLVEWPDLIIGKFSNEFLDLPVEVLSTVMKIHQRYVPLLLKNESFSKLDLSSEKNISTTFCVISNGLEE
SNNNIAKGNEKVLRARFSDAKFFVESDKKVASIERNEKLKSVSYLKGLGNIFQRVERIEEVTKKILKFLNDKSLEEKKII
EAAKYCKNDLCSEIVFEFPELQGIMGAKYLKYEGFSEDVCLAVAEHYLPSFYKDALPSTKYGAIVSIADKVETLISIFIS
GKRPSGSSDPYALRRNLNGVIKIIWDYEFDLPLDKLFNELIDFWKIAFPNLNFSREKVFNDLNEFLVQRIVSHLEEISLS
KELIRAVCSSDELSKKRVLNIVDLKNRIKSIMDFNEKENFVEIQKVITRASKLANNSDLSTDVLATRDYVNTKLFEKDCE
LKVFEFIEELEKLFSEDYCNYLELLNLFEINVNTIEDLFDNEKGVLIMSEDLKIRNNRLNLLSLIRNYSLKIADFTLLNS
>Mature_719_residues
SKYLLEIGTEELPAKFSYSVLEQFKSLIEFELDKKLIKFEHIVVTSTPRRIVLLLEGLVDYAEDKIIERKGPKANSAYLN
GCPTNAALGFANSLDINVGELEIKNTEKGDFVFGKKIEKGLSTKISLSSIIPKLVKSLQGARFMKWGAGNMKFSRPIRWI
ASIYNDEILDFEFDECDPKIKISNKTKSHRLINEVLEVQNPDEFFELLKRNRVIAIRKERKEKIESLINQASKSLNLEPD
LSEGLLNELTDLVEWPDLIIGKFSNEFLDLPVEVLSTVMKIHQRYVPLLLKNESFSKLDLSSEKNISTTFCVISNGLEES
NNNIAKGNEKVLRARFSDAKFFVESDKKVASIERNEKLKSVSYLKGLGNIFQRVERIEEVTKKILKFLNDKSLEEKKIIE
AAKYCKNDLCSEIVFEFPELQGIMGAKYLKYEGFSEDVCLAVAEHYLPSFYKDALPSTKYGAIVSIADKVETLISIFISG
KRPSGSSDPYALRRNLNGVIKIIWDYEFDLPLDKLFNELIDFWKIAFPNLNFSREKVFNDLNEFLVQRIVSHLEEISLSK
ELIRAVCSSDELSKKRVLNIVDLKNRIKSIMDFNEKENFVEIQKVITRASKLANNSDLSTDVLATRDYVNTKLFEKDCEL
KVFEFIEELEKLFSEDYCNYLELLNLFEINVNTIEDLFDNEKGVLIMSEDLKIRNNRLNLLSLIRNYSLKIADFTLLNS

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=725, Percent_Identity=30.4827586206897, Blast_Score=321, Evalue=8e-89,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 82781; Mature: 82650

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKYLLEIGTEELPAKFSYSVLEQFKSLIEFELDKKLIKFEHIVVTSTPRRIVLLLEGLV
CCCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHH
DYAEDKIIERKGPKANSAYLNGCPTNAALGFANSLDINVGELEIKNTEKGDFVFGKKIEK
HHHHHHHHHCCCCCCCCCEECCCCCCHHHCCCCCCCCCCCEEEECCCCCCCEEEHHHHHH
GLSTKISLSSIIPKLVKSLQGARFMKWGAGNMKFSRPIRWIASIYNDEILDFEFDECDPK
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
IKISNKTKSHRLINEVLEVQNPDEFFELLKRNRVIAIRKERKEKIESLINQASKSLNLEP
EEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCC
DLSEGLLNELTDLVEWPDLIIGKFSNEFLDLPVEVLSTVMKIHQRYVPLLLKNESFSKLD
CHHHHHHHHHHHHHHCHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHCCCEECCCCCCCCC
LSSEKNISTTFCVISNGLEESNNNIAKGNEKVLRARFSDAKFFVESDKKVASIERNEKLK
CCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
SVSYLKGLGNIFQRVERIEEVTKKILKFLNDKSLEEKKIIEAAKYCKNDLCSEIVFEFPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHH
LQGIMGAKYLKYEGFSEDVCLAVAEHYLPSFYKDALPSTKYGAIVSIADKVETLISIFIS
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
GKRPSGSSDPYALRRNLNGVIKIIWDYEFDLPLDKLFNELIDFWKIAFPNLNFSREKVFN
CCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
DLNEFLVQRIVSHLEEISLSKELIRAVCSSDELSKKRVLNIVDLKNRIKSIMDFNEKENF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VEIQKVITRASKLANNSDLSTDVLATRDYVNTKLFEKDCELKVFEFIEELEKLFSEDYCN
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YLELLNLFEINVNTIEDLFDNEKGVLIMSEDLKIRNNRLNLLSLIRNYSLKIADFTLLNS
HHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCEECCCHHHHHHHHHHCCCEEEEEEEECC
>Mature Secondary Structure 
SKYLLEIGTEELPAKFSYSVLEQFKSLIEFELDKKLIKFEHIVVTSTPRRIVLLLEGLV
CCCHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHH
DYAEDKIIERKGPKANSAYLNGCPTNAALGFANSLDINVGELEIKNTEKGDFVFGKKIEK
HHHHHHHHHCCCCCCCCCEECCCCCCHHHCCCCCCCCCCCEEEECCCCCCCEEEHHHHHH
GLSTKISLSSIIPKLVKSLQGARFMKWGAGNMKFSRPIRWIASIYNDEILDFEFDECDPK
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
IKISNKTKSHRLINEVLEVQNPDEFFELLKRNRVIAIRKERKEKIESLINQASKSLNLEP
EEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCCC
DLSEGLLNELTDLVEWPDLIIGKFSNEFLDLPVEVLSTVMKIHQRYVPLLLKNESFSKLD
CHHHHHHHHHHHHHHCHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHCCCEECCCCCCCCC
LSSEKNISTTFCVISNGLEESNNNIAKGNEKVLRARFSDAKFFVESDKKVASIERNEKLK
CCCCCCCCEEEEEEECCHHHCCCCCCCCCHHHHHHHHCCCCEEEECCCHHHHHHHHHHHH
SVSYLKGLGNIFQRVERIEEVTKKILKFLNDKSLEEKKIIEAAKYCKNDLCSEIVFEFPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHH
LQGIMGAKYLKYEGFSEDVCLAVAEHYLPSFYKDALPSTKYGAIVSIADKVETLISIFIS
HHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
GKRPSGSSDPYALRRNLNGVIKIIWDYEFDLPLDKLFNELIDFWKIAFPNLNFSREKVFN
CCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
DLNEFLVQRIVSHLEEISLSKELIRAVCSSDELSKKRVLNIVDLKNRIKSIMDFNEKENF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
VEIQKVITRASKLANNSDLSTDVLATRDYVNTKLFEKDCELKVFEFIEELEKLFSEDYCN
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
YLELLNLFEINVNTIEDLFDNEKGVLIMSEDLKIRNNRLNLLSLIRNYSLKIADFTLLNS
HHHHHHHHHCCHHHHHHHHCCCCCEEEEECCCEECCCHHHHHHHHHHCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA