Click here to switch to the map view.

The map label for this gene is met17 [H]

Identifier: 126696007

GI number: 126696007

Start: 595232

End: 596560

Strand: Direct

Name: met17 [H]

Synonym: P9301_06691

Alternate gene names: 126696007

Gene position: 595232-596560 (Clockwise)

Preceding gene: 126696006

Following gene: 126696008

Centisome position: 36.25

GC content: 38.53

Gene sequence:

>1329_bases
TTGAGCAACCAAAAATTCGAGACGCTTCAGTTACATGCAGGCCAAGTGCCTGATCCAACTACAAATTCTAGAGCAGTTCC
CATTTATCAAACTAGTTCCTATGTTTTTGATAATGCCGAGCATGGCGCGAATCTTTTTGGATTAAAAGAATTTGGAAATA
TTTATACTCGACTTATGAATCCCACCACAGATGTCTTTGAAAAAAGGATGGCAGCTTTGGAGGGAGGGATGGCAGCACTT
GCAACATCCTCAGGGCAAGCTGCTCAATTCTTAGCAATCGTGAACTGCATGACAGCGGGGGATAATTTTGTCTCTACATC
TTTTCTATATGGTGGGACCTACAATCAATTCAAAGTACAATTTCCAAGATTAGGAATAGAAGTTAAATTTGCAGATGGTG
ATAGTATAGATAGTTTTAGAAATAAAATTGATGATAAAACAAAAGCAATATATGTCGAATCAATGGGAAATCCTAGGTTC
AATATTCCAGATTTTGAGGGACTCTCTGCTTTGGCGAAGGAAAATGGAATTCCTCTAATAGTGGATAATACCCTTGGTGC
TGGTGGTGCTTTAATAAGACCAATTGATTTTGGAGCCGATGTTGTTGTAGAGAGTGCAACGAAATGGATTGGTGGACATG
GAACAAGCATCGGTGGGGTTATTGTCGATGCAGGAACCTTTGATTGGGGAAATGGTAAATTCCCTCTAATGAGTCAGCCA
AGCGCTGCTTATCATGGACTCGTTCATTGGGACGCATTTGGTTTCGGTAGTGATATCTGCAAATCTTTGGGAGTACCTGA
TAATAGAAATATAGCTTTTGCTTTGAGAGCAAGACTTGAATGCCTTAGAGACTGGGGATCAGCTCAAAGTCCTTTTAATT
CGTTTTTGCTTTTGCAGGGTTTGGAAACTCTAAGCTTAAGGATAGAAAGACAAACTTCTAATGCTCTTGAATTAGCAAAA
TGGTTAGATTCTAATTCTAATGTAAGTAGTGTTAATTATCCTGGCCTAGAATCTGATCCATATTACTCAAGTGCCAAAAA
ATATACTACTGGAAGGGGAATGGGTTGCATGCTTATGTTCTCTCTTAATGGAGGTTATGAAAATGCAGTAAAATTTATTG
ATTCCTTAAAATTAGCGAGTCACCTTGCTAATGTAGGTGATTCAAAAACTTTAGTAATTCATCCGGCTTCAACTACTCAT
CAGCAATTATCTGAAGAAGAACAATTATCTGCAGGCGTTACCCCCACGATGGTAAGAGTTTCTGTAGGAATTGAACATAT
TGATGATATTAAAGCCGATTTCGAACAAGCACTTTCACAAATCACATAG

Upstream 100 bases:

>100_bases
TTACCCCTCATTAAAAAAGGATTTATAAATATTTATCTTTTTTTTGTTTTTTTTTGGAACTTTTTTGAGTTAAATAGATA
ATAGGATCATTTAAAAAATT

Downstream 100 bases:

>100_bases
GAAAGGAGATTTATTGGCTTTAATAATTCCTGGTAATTATCACAAGATTAGTGATGTTGAGAAAAATCATATATCTTGGA
TCGAACCAGAATTGGCAAAA

Product: putative O-Acetyl homoserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAAL
ATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQFPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRF
NIPDFEGLSALAKENGIPLIVDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSQP
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQGLETLSLRIERQTSNALELAK
WLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMFSLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTH
QQLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALSQIT

Sequences:

>Translated_442_residues
MSNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAAL
ATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQFPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRF
NIPDFEGLSALAKENGIPLIVDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSQP
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQGLETLSLRIERQTSNALELAK
WLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMFSLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTH
QQLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALSQIT
>Mature_441_residues
SNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMNPTTDVFEKRMAALEGGMAALA
TSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQFPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRFN
IPDFEGLSALAKENGIPLIVDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSQPS
AAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQGLETLSLRIERQTSNALELAKW
LDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMFSLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTHQ
QLSEEEQLSAGVTPTMVRVSVGIEHIDDIKADFEQALSQIT

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=439, Percent_Identity=30.0683371298405, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI299473759, Length=439, Percent_Identity=27.1070615034169, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI25453487, Length=438, Percent_Identity=27.1689497716895, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1790375, Length=433, Percent_Identity=28.8683602771363, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789383, Length=446, Percent_Identity=21.7488789237668, Blast_Score=102, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=455, Percent_Identity=29.6703296703297, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=438, Percent_Identity=32.1917808219178, Blast_Score=181, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=437, Percent_Identity=30.6636155606407, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323333, Length=451, Percent_Identity=53.6585365853659, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6319307, Length=444, Percent_Identity=31.0810810810811, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=464, Percent_Identity=25.6465517241379, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321170, Length=332, Percent_Identity=25, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922554, Length=438, Percent_Identity=29.2237442922374, Blast_Score=170, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47853; Mature: 47722

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMN
CCCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRMAALEGGMAALATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRFNIPDFEGLSALAKENGIPLI
ECCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
VDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSQP
EECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHC
LETLSLRIERQTSNALELAKWLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMF
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
SLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTHQQLSEEEQLSAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHCCCCCEEEEE
SVGIEHIDDIKADFEQALSQIT
EECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNQKFETLQLHAGQVPDPTTNSRAVPIYQTSSYVFDNAEHGANLFGLKEFGNIYTRLMN
CCCCEEEEEEECCCCCCCCCCCEEEEEEECCCEEECCCCCCCCEEEHHHHHHHHHHHCC
PTTDVFEKRMAALEGGMAALATSSGQAAQFLAIVNCMTAGDNFVSTSFLYGGTYNQFKVQ
CHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEE
FPRLGIEVKFADGDSIDSFRNKIDDKTKAIYVESMGNPRFNIPDFEGLSALAKENGIPLI
ECCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEE
VDNTLGAGGALIRPIDFGADVVVESATKWIGGHGTSIGGVIVDAGTFDWGNGKFPLMSQP
EECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCEEECCCCCCCCCCCC
SAAYHGLVHWDAFGFGSDICKSLGVPDNRNIAFALRARLECLRDWGSAQSPFNSFLLLQG
CHHHCCEEEEECCCCHHHHHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHC
LETLSLRIERQTSNALELAKWLDSNSNVSSVNYPGLESDPYYSSAKKYTTGRGMGCMLMF
CCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
SLNGGYENAVKFIDSLKLASHLANVGDSKTLVIHPASTTHQQLSEEEQLSAGVTPTMVRV
EECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHCHHHHHHCCCCCEEEEE
SVGIEHIDDIKADFEQALSQIT
EECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]