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The map label for this gene is yafV [C]

Identifier: 126695979

GI number: 126695979

Start: 570264

End: 571091

Strand: Direct

Name: yafV [C]

Synonym: P9301_06411

Alternate gene names: 126695979

Gene position: 570264-571091 (Clockwise)

Preceding gene: 126695978

Following gene: 126695980

Centisome position: 34.73

GC content: 36.84

Gene sequence:

>828_bases
TTGACTGATTTTTTGGTAGCTGCATTGCAAATTACAAGTACTTCAAATGTTGAAGCGAATTTCATTGAAGCAGAAGAACA
GATTGAATTAGCTGCTAGAAGAGGTGCTGAGTTAATTGGATTGCCCGAGAATTTTGCTTTTTTAGGAGGAGATGATGAAA
AACTTAGATTAGCTTCGGAATTGTCAGAAAAGTGTGCAAATTTGCTAAAAACTATGTCACAAAGGTATCAAGTATTTCTT
TTGGGAGGGGGTTATCCTGTCCCTGCTGGTGATGACAGTCATACTTTTAATAGATCAGCACTATTTGGTAAAGATGGACA
GGTTTTGGCAAAATATGACAAAATTCATTTGTTTGATGTTGATTTGCCAGATGGAAATTTATATAAGGAATCATCCACTA
TTTTATCTGGGAAAGAGTATCCGCCTGTTGTAGATATTCCGGGCTTATGCAAAATAGGATTATCAATTTGTTATGACGTT
AGATTTCCTGAACTCTATAGATATTTGTCTTCAAATGGTGCAGAACTAATTATGATTCCTGCAGCTTTTACAGCATTTAC
TGGAAAAGATCATTGGCAAATCCTATTACAAGCAAGAGCAATTGAGAATACTGCATATGTAGTCGCTCCAGCTCAAACTG
GGATTCATTATGGGAGAAGGCAAAGTCATGGCCATGCAATGGTAATTGACCCTTGGGGTACAGTTTTATCTGATGCCGGA
AAAACTCAGGGAGCTGCAATAGCTCCTGCTGATAAAGAAAGAGTAAAGAAAATAAGGGAGCAGATGCCAAGCCTTAAACA
TAGGAAAAATAAGTTGTTTTCAAAATAA

Upstream 100 bases:

>100_bases
TTGCTCTGAACTTGATTGCTTAGATATTGTTCCAGCTCAAGTTGAAAGAGGTGTGATTCGTGCCTCATGATTTACGAATT
GGTTCACTAGGAGTAAAGTC

Downstream 100 bases:

>100_bases
TGATAAAGTTTTTAGATAATAAACTTTTTCGTTATTTATCCGTTTTTTTATTTTTAAATTCTTTAATCCTTCCAGTTAAA
TCGTCAAGTGCTCTGGCGGC

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTDFLVAALQITSTSNVEANFIEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASELSEKCANLLKTMSQRYQVFL
LGGGYPVPAGDDSHTFNRSALFGKDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGKEYPPVVDIPGLCKIGLSICYDV
RFPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSK

Sequences:

>Translated_275_residues
MTDFLVAALQITSTSNVEANFIEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASELSEKCANLLKTMSQRYQVFL
LGGGYPVPAGDDSHTFNRSALFGKDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGKEYPPVVDIPGLCKIGLSICYDV
RFPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSK
>Mature_274_residues
TDFLVAALQITSTSNVEANFIEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASELSEKCANLLKTMSQRYQVFLL
GGGYPVPAGDDSHTFNRSALFGKDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGKEYPPVVDIPGLCKIGLSICYDVR
FPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAGK
TQGAAIAPADKERVKKIREQMPSLKHRKNKLFSK

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=273, Percent_Identity=39.1941391941392, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI297632350, Length=273, Percent_Identity=39.1941391941392, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI5031947, Length=273, Percent_Identity=39.1941391941392, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI9910460, Length=274, Percent_Identity=33.2116788321168, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=37.3056994818653, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1786412, Length=249, Percent_Identity=28.5140562248996, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=272, Percent_Identity=39.7058823529412, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6322335, Length=302, Percent_Identity=33.112582781457, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323383, Length=281, Percent_Identity=31.3167259786477, Blast_Score=145, Evalue=5e-36,
Organism=Drosophila melanogaster, GI17933642, Length=271, Percent_Identity=35.4243542435424, Blast_Score=165, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21355835, Length=275, Percent_Identity=31.6363636363636, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30319; Mature: 30188

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLVAALQITSTSNVEANFIEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASE
CCCCEEEEEEEECCCCCCEEEECHHHHHHHHHHCCCEEEECCCCCEEECCCCHHHHHHHH
LSEKCANLLKTMSQRYQVFLLGGGYPVPAGDDSHTFNRSALFGKDGQVLAKYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCEECCCCCCCCCCCCCEEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGKEYPPVVDIPGLCKIGLSICYDVRFPELYRYLSSNGAELIMIP
ECCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCHHEEEEEHHHCCCCEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSK
CCCCCEECCCCHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
TDFLVAALQITSTSNVEANFIEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASE
CCCEEEEEEEECCCCCCEEEECHHHHHHHHHHCCCEEEECCCCCEEECCCCHHHHHHHH
LSEKCANLLKTMSQRYQVFLLGGGYPVPAGDDSHTFNRSALFGKDGQVLAKYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCEECCCCCCCCCCCCCEEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGKEYPPVVDIPGLCKIGLSICYDVRFPELYRYLSSNGAELIMIP
ECCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCHHEEEEEHHHCCCCEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSK
CCCCCEECCCCHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]