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The map label for this gene is gltX [H]

Identifier: 126695836

GI number: 126695836

Start: 434230

End: 435660

Strand: Reverse

Name: gltX [H]

Synonym: P9301_04981

Alternate gene names: 126695836

Gene position: 435660-434230 (Counterclockwise)

Preceding gene: 126695837

Following gene: 126695832

Centisome position: 26.53

GC content: 32.08

Gene sequence:

>1431_bases
TTGGAAAAACGTTTAAGACTAGCCCCGAGTCCAACGGGTTTATTTCATATTGGGACAGCACGAACAGCACTATTCAATTG
GTTGTATGCACAAAAAATAGGTGGAAAATTTCTTATCAGAATAGAAGATACAGATTTTCTTCGATCTAAATCTGAATATA
CAAAAAATATATTAGAAGGCTTGAAATGGCTTGGGCTTAAATGGGATGAAGAACCTATAAAGCAAAGTGACCGAATTTCG
ATTCACAAAAGTTATATCAAAAAACTATTGGAATGTGGAGCTGCATATAGGTGCTTTACATCAGAAGATGAAATATCTGA
ATTAAGAGAAGAACAAAAAAAGAAAGGATTACCTCCAAAGCATGATAATAGACACAGAAGTCTTTCAAAAGAAGAAATAG
AAACATTCATATCCCAAGGGCGGACTTCGGTAATAAGATTTAAGATTGATGAAAAAATTGAAATTAAATGGATAGATCAG
ATAAGAGGCGAAATCAAATGGCAAGGTAAGGATTTGGGCGGTGATTTAGTTTTATCAAGAAGGGCTAAGGGATATGAGAT
TGGTGATCCTTTGTATAATCTTGCAGTAGTAGTTGATGATAATTTCATGAATATTACTCACGTTGTAAGGGGTGAAGACC
ATATCTCGAACACTGCAAAACAAATATTGATTTATAAAGCATTAAATTTTAATTTACCAACTTTTTCGCATACACCCTTA
ATACTGAATAGCGAAGGGAAAAAATTATCTAAGAGAGATTGCGTTACTTCAATCGACGAATTTAGAGAAATGGGATATTT
ACCTGAGGCCCTATCGAACTATATGGCATTTTTAGGTTGGTCTCCAAAATCTGCCGACAGAGAAATACTTTCTCTTGAAG
AGATATCTAAAATTTTTGACTTATCAGAAATAAATAAAGCTGGAGCCAAATTTAGTTGGGAAAAACTCAACTGGATTAAT
TCTCAATATATAAAAAATATTGAATCAATTAAGTTAATTGAGATCATGAGAAAATACTGGGACGACAATGGTTGGAAGCC
GCCATCTGAAGAATGGGCTAATAAATTAGCAATTTTGATTAGAGACTCTATGACTCTTTTAAAAGATTCAATTGATCAAT
CAAAACCATTTTTCTTGATACCTACAATTCAAAAAGAAGGTCAAGATTTTCTGGAAATCCGGGAAAGCAAATTATCTTTA
AAACTAATCTTAAATTATTTAATTGAGAAAAATACTATAAAACTAAATAAAGAGAAAGCCAAAGAAATAATAAACGAAAT
CTCAAAAAAGCATAATATTAAAAAAGGGATATTAATGAAATCACTAAGAGTAGCCTTTTTTGGATCTCTCAGTGGGCCAG
ATTTAATTCAAAGTTGGGAGCTTTTCGCAGAAAGTAAAACTGATAGAACTCGAATTGAAAGATGTCTTTAA

Upstream 100 bases:

>100_bases
CAAAACTCTGGGACCGTAGTTCAACTGGTTAGAGCACCGCCCTGTCACGGCGGAAGTTGCGGGTTCGAATCCCGTCGGTC
CCGTTTTTTCTAAAAGAAAT

Downstream 100 bases:

>100_bases
TCAATCAAAATTATTTTTTTGGCCTAATTTAAGTCTATTTACCAAAGGTTTAGCTGAAAGTCCTTGTATTCCTACTGTCA
TCAAAATAGTAAGAAAAACT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILEGLKWLGLKWDEEPIKQSDRIS
IHKSYIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPKHDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIEIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYKALNFNLPTFSHTPL
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKSADREILSLEEISKIFDLSEINKAGAKFSWEKLNWIN
SQYIKNIESIKLIEIMRKYWDDNGWKPPSEEWANKLAILIRDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLEIRESKLSL
KLILNYLIEKNTIKLNKEKAKEIINEISKKHNIKKGILMKSLRVAFFGSLSGPDLIQSWELFAESKTDRTRIERCL

Sequences:

>Translated_476_residues
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILEGLKWLGLKWDEEPIKQSDRIS
IHKSYIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPKHDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIEIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYKALNFNLPTFSHTPL
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKSADREILSLEEISKIFDLSEINKAGAKFSWEKLNWIN
SQYIKNIESIKLIEIMRKYWDDNGWKPPSEEWANKLAILIRDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLEIRESKLSL
KLILNYLIEKNTIKLNKEKAKEIINEISKKHNIKKGILMKSLRVAFFGSLSGPDLIQSWELFAESKTDRTRIERCL
>Mature_476_residues
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILEGLKWLGLKWDEEPIKQSDRIS
IHKSYIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPKHDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIEIKWIDQ
IRGEIKWQGKDLGGDLVLSRRAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYKALNFNLPTFSHTPL
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKSADREILSLEEISKIFDLSEINKAGAKFSWEKLNWIN
SQYIKNIESIKLIEIMRKYWDDNGWKPPSEEWANKLAILIRDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLEIRESKLSL
KLILNYLIEKNTIKLNKEKAKEIINEISKKHNIKKGILMKSLRVAFFGSLSGPDLIQSWELFAESKTDRTRIERCL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=334, Percent_Identity=34.4311377245509, Blast_Score=208, Evalue=1e-53,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=35.6371490280778, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI226510925, Length=253, Percent_Identity=32.4110671936759, Blast_Score=125, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI71993394, Length=479, Percent_Identity=31.1064718162839, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=333, Percent_Identity=40.2402402402402, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=27.9475982532751, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=32.9268292682927, Blast_Score=241, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55324; Mature: 55324

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILEG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LKWLGLKWDEEPIKQSDRISIHKSYIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPK
HHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCC
HDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIEIKWIDQIRGEIKWQGKDLGGDLVLSR
CCCHHHCCCHHHHHHHHHCCCCEEEEEEECCEEEEEEHHHHCCEEEECCCCCCCHHEEEC
RAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYKALNFNLPTFSHTPL
CCCCCCCCCCCEEEEEEEECCCEEEHEEECCCHHHHHHHHHHHHEEEECCCCCCCCCCCE
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKSADREILSLEEISKIFD
EECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LSEINKAGAKFSWEKLNWINSQYIKNIESIKLIEIMRKYWDDNGWKPPSEEWANKLAILI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEHHH
RDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLEIRESKLSLKLILNYLIEKNTIKLNKEKA
HHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHH
KEIINEISKKHNIKKGILMKSLRVAFFGSLSGPDLIQSWELFAESKTDRTRIERCL
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MEKRLRLAPSPTGLFHIGTARTALFNWLYAQKIGGKFLIRIEDTDFLRSKSEYTKNILEG
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHH
LKWLGLKWDEEPIKQSDRISIHKSYIKKLLECGAAYRCFTSEDEISELREEQKKKGLPPK
HHHCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHCCCCCC
HDNRHRSLSKEEIETFISQGRTSVIRFKIDEKIEIKWIDQIRGEIKWQGKDLGGDLVLSR
CCCHHHCCCHHHHHHHHHCCCCEEEEEEECCEEEEEEHHHHCCEEEECCCCCCCHHEEEC
RAKGYEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIYKALNFNLPTFSHTPL
CCCCCCCCCCCEEEEEEEECCCEEEHEEECCCHHHHHHHHHHHHEEEECCCCCCCCCCCE
ILNSEGKKLSKRDCVTSIDEFREMGYLPEALSNYMAFLGWSPKSADREILSLEEISKIFD
EECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
LSEINKAGAKFSWEKLNWINSQYIKNIESIKLIEIMRKYWDDNGWKPPSEEWANKLAILI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHEEHHH
RDSMTLLKDSIDQSKPFFLIPTIQKEGQDFLEIRESKLSLKLILNYLIEKNTIKLNKEKA
HHHHHHHHHHHCCCCCEEEEECHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHH
KEIINEISKKHNIKKGILMKSLRVAFFGSLSGPDLIQSWELFAESKTDRTRIERCL
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA