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The map label for this gene is trmE

Identifier: 126695547

GI number: 126695547

Start: 191803

End: 193185

Strand: Reverse

Name: trmE

Synonym: P9301_02091

Alternate gene names: 126695547

Gene position: 193185-191803 (Counterclockwise)

Preceding gene: 126695549

Following gene: 126695546

Centisome position: 11.77

GC content: 27.48

Gene sequence:

>1383_bases
ATGGATTCGATAGTTACTACAGAAGATACGATAGCCGCAATTGCTTCAGCTATAAGTATTGGGAAGGGAGGAGTTGCGAT
AATAAGAGTATCAGGGAAAGACGCAATAAATTCTTGCAAAAAGATTGTTCAAACTAAATCTAAATATGCATGGGAATCAC
ATAGGGTTTTTCGTGGTTTTATTCAGGAAAATAAACAAAATAAATTTATAGATGAAGTTTTAATTTTAGTGATGAAATCA
CCAAATAGCTTCACAGGAGAGGATGTCGTTGAACTTCATTGTCATGGCGGGATTATCATAGTAAATAAAGTTTTAAAGAT
ATTATTATCTAGTAATTCTAGAGTTAGAATTGCAAACCCAGGAGAATTTAGTCAAAGAGCTTTTCTTAATGGGAAAATAG
ACCTTACTCAAGCCGAGTCGATTAATCAATTAATTAATGCAAGCAATACCAGATCAGCAGAGTTAGCCTTTAGCGGAATT
CAAGGAGAAATAAAGAAAAAAATTGATGATATTAAAAATGACCTTATAAATCAACTTTGCGAAATAGAAGCGAGAGTTGA
TTTTGAAGAAGACTTCACAGATTTTGATTACACCAAATATCTAAAAAACATTAAAAAAGTCAAAGAAAAAATAGAATTAC
TAATTGAAAATGCAAAAAGAAATTCATATATTCACAATGGGATATCCATTGCGCTTATAGGTAAAACAAATGTTGGTAAA
AGCTCTTTATTAAATTTGCTTGCAAAAAAAGAGAAAGCAATCGTAACTAATATTCCTGGAACAACTAGAGATGTTATTGA
AGTTAATTTAACTATTAATGATATTCCAATGAAAATAATTGATACTGCTGGCATAAGAGAAACTTATGAACAAATTGAAA
GTATTGGAATTAAAAAAAGTTTTGGGAAAATAAAAGAGTCAGATTTTATAATTTATATTTATAGTCTTGAAGAAGGATTT
AATGAAGAAGACAAAAAAATAATACAAGAAATCCCCAAAGAAAAATTAATTACTATTTTGGGCAATAAAAAAGATTTAAT
TGATTGCAAAAATATTAATTCAAATGAGCTAAAAAACACAATTCTTATGAGTATTAAGAATAATGATGGTGAAAAATTAT
TAATAGACACAATCATAAAAAAATGCGGATTAAAACAAGTAGAAAATATTAATATATTTTTAAACGAAAGACATCAAACA
AATTTGTCTGCTTGCTTATCTAATTTAAATGATACTGATGAAATAATTGAAAATAAATTGCCATTTGATTTGTTATCAAT
TGAACTGAGAGATGGAATTCAAAACTTATCTAAAATAACTGGTCAAGAATTAACAGAGGAACTTCTAGATAATATTTTTT
CTAAGTTTTGTATTGGCAAATAA

Upstream 100 bases:

>100_bases
TTTTCTTATAGGTAATATCTCTTTTAAATCTCATTCAGAAAGAAAATCTCAAAATTAAATTCTTGATAATCTTACTTAAT
TATCCATAACAAAGCGAGAG

Downstream 100 bases:

>100_bases
ATTTGAGTTAAATTACATAGTGATATATAGTTGAATGTCTAACTTCAGTTTAATTTTTATTAATTATTTCTTAGTTTAGT
GGATTTAAATACTCCAATAA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGFIQENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIIIVNKVLKILLSSNSRVRIANPGEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGI
QGEIKKKIDDIKNDLINQLCEIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETYEQIESIGIKKSFGKIKESDFIIYIYSLEEGF
NEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNTILMSIKNNDGEKLLIDTIIKKCGLKQVENINIFLNERHQT
NLSACLSNLNDTDEIIENKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK

Sequences:

>Translated_460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGFIQENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIIIVNKVLKILLSSNSRVRIANPGEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGI
QGEIKKKIDDIKNDLINQLCEIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETYEQIESIGIKKSFGKIKESDFIIYIYSLEEGF
NEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNTILMSIKNNDGEKLLIDTIIKKCGLKQVENINIFLNERHQT
NLSACLSNLNDTDEIIENKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
>Mature_460_residues
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGFIQENKQNKFIDEVLILVMKS
PNSFTGEDVVELHCHGGIIIVNKVLKILLSSNSRVRIANPGEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGI
QGEIKKKIDDIKNDLINQLCEIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETYEQIESIGIKKSFGKIKESDFIIYIYSLEEGF
NEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNTILMSIKNNDGEKLLIDTIIKKCGLKQVENINIFLNERHQT
NLSACLSNLNDTDEIIENKLPFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=29.3103448275862, Blast_Score=211, Evalue=9e-55,
Organism=Homo sapiens, GI193082993, Length=454, Percent_Identity=30.1762114537445, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=27.6209677419355, Blast_Score=203, Evalue=3e-52,
Organism=Escherichia coli, GI2367268, Length=461, Percent_Identity=33.6225596529284, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI87082120, Length=120, Percent_Identity=32.5, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=28.5714285714286, Blast_Score=159, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6323665, Length=504, Percent_Identity=31.547619047619, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=29.957805907173, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PROM0 (A3PAQ7)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090433.1
- ProteinModelPortal:   A3PAQ7
- SMR:   A3PAQ7
- STRING:   A3PAQ7
- GeneID:   4912085
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_02091
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   PMAR167546:P9301ORF_0211-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00449
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51651; Mature: 51651

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: BINDING 29-29 BINDING 91-91 BINDING 132-132 BINDING 460-460

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGF
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQENKQNKFIDEVLILVMKSPNSFTGEDVVELHCHGGIIIVNKVLKILLSSNSRVRIANP
HHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEECCC
GEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGIQGEIKKKIDDIKNDLINQLC
CCCCHHHEECCCEEEHHHHHHHHHHCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
HHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCH
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETYEQIESIGIKKS
HHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHH
FGKIKESDFIIYIYSLEEGFNEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNT
HCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHEEEEECCCCCEEECCCCCHHHHCCE
ILMSIKNNDGEKLLIDTIIKKCGLKQVENINIFLNERHQTNLSACLSNLNDTDEIIENKL
EEEEEECCCCCEEHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHCCC
PFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDSIVTTEDTIAAIASAISIGKGGVAIIRVSGKDAINSCKKIVQTKSKYAWESHRVFRGF
CCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQENKQNKFIDEVLILVMKSPNSFTGEDVVELHCHGGIIIVNKVLKILLSSNSRVRIANP
HHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCEEEHHHHHHHHHCCCCEEEECCC
GEFSQRAFLNGKIDLTQAESINQLINASNTRSAELAFSGIQGEIKKKIDDIKNDLINQLC
CCCCHHHEECCCEEEHHHHHHHHHHCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHHHHH
EIEARVDFEEDFTDFDYTKYLKNIKKVKEKIELLIENAKRNSYIHNGISIALIGKTNVGK
HHHHCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEECCCCCH
SSLLNLLAKKEKAIVTNIPGTTRDVIEVNLTINDIPMKIIDTAGIRETYEQIESIGIKKS
HHHHHHHHHHCCEEEECCCCCCCEEEEEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHH
FGKIKESDFIIYIYSLEEGFNEEDKKIIQEIPKEKLITILGNKKDLIDCKNINSNELKNT
HCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHEEEEECCCCCEEECCCCCHHHHCCE
ILMSIKNNDGEKLLIDTIIKKCGLKQVENINIFLNERHQTNLSACLSNLNDTDEIIENKL
EEEEEECCCCCEEHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHCCCCHHHHHHCCC
PFDLLSIELRDGIQNLSKITGQELTEELLDNIFSKFCIGK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA