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The map label for this gene is ppnK [H]

Identifier: 126695514

GI number: 126695514

Start: 163350

End: 164261

Strand: Reverse

Name: ppnK [H]

Synonym: P9301_01761

Alternate gene names: 126695514

Gene position: 164261-163350 (Counterclockwise)

Preceding gene: 126695515

Following gene: 126695513

Centisome position: 10.0

GC content: 30.15

Gene sequence:

>912_bases
ATGAAACTCTCATTAGTGCTTATTGTATATCGTTCAGATAGTTCTATCGCTAAAGAGGCTTCTAAATTCTGTGAAGAAGT
CCTCAAAGCTAAAAACATAAAATCAAAAAGAATTGAAAGTGATTTTTATAAAGATGAAATTGAAAAATATTTTTGTAATA
AGGAATTAAAACCAAATATTGGCATAGTTCTTGGTGGAGATGGAACCTTTCTAAAATGTGCAAATGCTTTAGCTGATTAT
GATATTCCTTTGTTGAGCATTAATATTGGTGGAAATCTGGGTTTTCTTACACAAGAAAAAGATTTTTTGTTTGATAAATC
TTTTATTGAAATACTTGAAAAAGAAGAATATACAATTGACTTTCGTAATAGATTAAACTGTAATGTCTGTATTAATGGGA
CAATTTCTGAGAAAAAGATAATAAAAAGTTTCGATGCGTTAAATGATTTTTATTTTAAATCTGTTGAAGAAGACATTTCT
CCAACCAATCAAATACAGATTGAAATAGATAACGAGAAGGTTAACGAATATAAAGGTGATGGATTAATCATATCTACATC
TACTGGCTCAACAGCCTACTCAATGGCTGCAGGGGGGCCAATAGTGCACCCAAGTATTGATGCGATGATAATTAACCCTA
TATGCCCGATGAGTTTGGCTAGTAGACCAATAGTCATACCTAATACAAGTAAGGTAATAATCAAACCTGTAAAAAAAAGT
AAAGGGGAAATTAAATTATGGAGAGATGGTTCAAAATGTATGACCATCAAGGAAAATTATTATTGTGAGATCAAAAAAGG
GCAATCACCCTGCAAAATAATAAAATTTAAAAAAAGCACTAATTACTACAATACCCTAATTAAAAAACTAGACTGGAAAG
GCGATTTATCTCAAAAATATCCAAAACATTAA

Upstream 100 bases:

>100_bases
AGAAGCTGCTGTAGGGTTAGCTATCTTATTATCTCTTTATAGGAATAGGGTGACTGTAGATATGGAAAGTTTTAATTTAT
TAAAATGGTAAAACCACTAA

Downstream 100 bases:

>100_bases
ATGGCCTTAGAGATAGAAAGAAGATTTCTTATAAAAAATGATAATTGGAAAGAATTCATAAATAAAAAAATTCCTATTGA
ACAAGGATATTTATCCAACA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1 [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKLSLVLIVYRSDSSIAKEASKFCEEVLKAKNIKSKRIESDFYKDEIEKYFCNKELKPNIGIVLGGDGTFLKCANALADY
DIPLLSINIGGNLGFLTQEKDFLFDKSFIEILEKEEYTIDFRNRLNCNVCINGTISEKKIIKSFDALNDFYFKSVEEDIS
PTNQIQIEIDNEKVNEYKGDGLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
KGEIKLWRDGSKCMTIKENYYCEIKKGQSPCKIIKFKKSTNYYNTLIKKLDWKGDLSQKYPKH

Sequences:

>Translated_303_residues
MKLSLVLIVYRSDSSIAKEASKFCEEVLKAKNIKSKRIESDFYKDEIEKYFCNKELKPNIGIVLGGDGTFLKCANALADY
DIPLLSINIGGNLGFLTQEKDFLFDKSFIEILEKEEYTIDFRNRLNCNVCINGTISEKKIIKSFDALNDFYFKSVEEDIS
PTNQIQIEIDNEKVNEYKGDGLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
KGEIKLWRDGSKCMTIKENYYCEIKKGQSPCKIIKFKKSTNYYNTLIKKLDWKGDLSQKYPKH
>Mature_303_residues
MKLSLVLIVYRSDSSIAKEASKFCEEVLKAKNIKSKRIESDFYKDEIEKYFCNKELKPNIGIVLGGDGTFLKCANALADY
DIPLLSINIGGNLGFLTQEKDFLFDKSFIEILEKEEYTIDFRNRLNCNVCINGTISEKKIIKSFDALNDFYFKSVEEDIS
PTNQIQIEIDNEKVNEYKGDGLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
KGEIKLWRDGSKCMTIKENYYCEIKKGQSPCKIIKFKKSTNYYNTLIKKLDWKGDLSQKYPKH

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Homo sapiens, GI55743112, Length=326, Percent_Identity=26.0736196319018, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788968, Length=238, Percent_Identity=34.0336134453782, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320794, Length=257, Percent_Identity=29.5719844357977, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322509, Length=302, Percent_Identity=26.4900662251656, Blast_Score=97, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325068, Length=175, Percent_Identity=34.2857142857143, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28573830, Length=252, Percent_Identity=28.1746031746032, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28573828, Length=252, Percent_Identity=28.1746031746032, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI161077047, Length=252, Percent_Identity=28.1746031746032, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28573832, Length=252, Percent_Identity=28.1746031746032, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI28573826, Length=252, Percent_Identity=28.1746031746032, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281363323, Length=334, Percent_Identity=24.8502994011976, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363321, Length=334, Percent_Identity=24.8502994011976, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129957, Length=334, Percent_Identity=24.8502994011976, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653424, Length=334, Percent_Identity=24.8502994011976, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24653422, Length=334, Percent_Identity=24.8502994011976, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 34285; Mature: 34285

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSLVLIVYRSDSSIAKEASKFCEEVLKAKNIKSKRIESDFYKDEIEKYFCNKELKPNI
CCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
GIVLGGDGTFLKCANALADYDIPLLSINIGGNLGFLTQEKDFLFDKSFIEILEKEEYTID
EEEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCEEEE
FRNRLNCNVCINGTISEKKIIKSFDALNDFYFKSVEEDISPTNQIQIEIDNEKVNEYKGD
ECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCC
GLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
EEEEEECCCCCEEEECCCCCEECCCCCEEEECCCCCHHHCCCCEECCCCCEEEEEECCCC
KGEIKLWRDGSKCMTIKENYYCEIKKGQSPCKIIKFKKSTNYYNTLIKKLDWKGDLSQKY
CCCEEEEECCCEEEEEECCEEEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCC
PKH
CCC
>Mature Secondary Structure
MKLSLVLIVYRSDSSIAKEASKFCEEVLKAKNIKSKRIESDFYKDEIEKYFCNKELKPNI
CCEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
GIVLGGDGTFLKCANALADYDIPLLSINIGGNLGFLTQEKDFLFDKSFIEILEKEEYTID
EEEECCCCCHHHHHHHHHCCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCEEEE
FRNRLNCNVCINGTISEKKIIKSFDALNDFYFKSVEEDISPTNQIQIEIDNEKVNEYKGD
ECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCC
GLIISTSTGSTAYSMAAGGPIVHPSIDAMIINPICPMSLASRPIVIPNTSKVIIKPVKKS
EEEEEECCCCCEEEECCCCCEECCCCCEEEECCCCCHHHCCCCEECCCCCEEEEEECCCC
KGEIKLWRDGSKCMTIKENYYCEIKKGQSPCKIIKFKKSTNYYNTLIKKLDWKGDLSQKY
CCCEEEEECCCEEEEEECCEEEEEECCCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCC
PKH
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA