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The map label for this gene is cysH [H]

Identifier: 126695431

GI number: 126695431

Start: 95956

End: 96678

Strand: Reverse

Name: cysH [H]

Synonym: P9301_00931

Alternate gene names: 126695431

Gene position: 96678-95956 (Counterclockwise)

Preceding gene: 126695436

Following gene: 126695430

Centisome position: 5.89

GC content: 31.81

Gene sequence:

>723_bases
ATGATTGAAAAAATCCACAAAAATATTCAAACTAACTTGATGAAATATAATCAAGAGCTTGTAAATATGAAGCCTCAAGA
AATTCTTACATGGGGTTATGAAAAGTTTGATGATCAATTTGCTATTACAACAAGTTTTGGTATACAGTCATCAGTCCTTT
TACATATGGTCAGCAAATTATCTCTACAAAAAAAAATCAAAATATTTTGGATAGATACAGGTTACCTACCTACAGAAACA
TACCATTACGCTGAAAATCTTATTGATCGTTTATCCTTAAAAGTTGAAGTTCTGCAAAGTGAATTATCTCCAGCAAGAAT
GGAGGCCAAATACGGAAAACTTTGGGAAACAAATAAAGTGAGTGATTTAGACAAGTATCATGAATTAAGAAAGATAAAAC
CTCTAGAAAATGGTCTAGAAAAATATAGTATTTATTGCTGGGCAAGCGGAGTTAGAGCAGGCCAAACAGAAACTAGAAAC
AAAATGAAATTCATAGACGTAATTCGTCAAAGACTCTCTTTAAGACCTTTATTAAATTGGACAAATAAAGATATTTTTTA
TTATATGGAAGAGAATAATTTACCTGCCCATCCACTTTTTATCAAAGGTTATTCTTCTGTAGGAGATTGGCATTCAAGCA
GTCCCGATGGTATGGAAACAAAGGGCAGAGATACAAGATTTGGAGGGATTAAACAAGAATGTGGAATACACACTAATAAT
TAA

Upstream 100 bases:

>100_bases
TTCATTAGAGAAATATTGTTATGCGTAAATGTATTATTAAACTATACGAATAATTTTTAAATTGAGCGAAATAAAATTAA
ACTCATAATCAAACTGAAGA

Downstream 100 bases:

>100_bases
ATTGATCATAGAACAATGGTCTCAGATATAAATTTTTTATTAGTAGGCAATAGTAGGCTTCATTGGGCAAAATATTCTAA
AAATCAATCTAAATTCTTCC

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MIEKIHKNIQTNLMKYNQELVNMKPQEILTWGYEKFDDQFAITTSFGIQSSVLLHMVSKLSLQKKIKIFWIDTGYLPTET
YHYAENLIDRLSLKVEVLQSELSPARMEAKYGKLWETNKVSDLDKYHELRKIKPLENGLEKYSIYCWASGVRAGQTETRN
KMKFIDVIRQRLSLRPLLNWTNKDIFYYMEENNLPAHPLFIKGYSSVGDWHSSSPDGMETKGRDTRFGGIKQECGIHTNN

Sequences:

>Translated_240_residues
MIEKIHKNIQTNLMKYNQELVNMKPQEILTWGYEKFDDQFAITTSFGIQSSVLLHMVSKLSLQKKIKIFWIDTGYLPTET
YHYAENLIDRLSLKVEVLQSELSPARMEAKYGKLWETNKVSDLDKYHELRKIKPLENGLEKYSIYCWASGVRAGQTETRN
KMKFIDVIRQRLSLRPLLNWTNKDIFYYMEENNLPAHPLFIKGYSSVGDWHSSSPDGMETKGRDTRFGGIKQECGIHTNN
>Mature_240_residues
MIEKIHKNIQTNLMKYNQELVNMKPQEILTWGYEKFDDQFAITTSFGIQSSVLLHMVSKLSLQKKIKIFWIDTGYLPTET
YHYAENLIDRLSLKVEVLQSELSPARMEAKYGKLWETNKVSDLDKYHELRKIKPLENGLEKYSIYCWASGVRAGQTETRN
KMKFIDVIRQRLSLRPLLNWTNKDIFYYMEENNLPAHPLFIKGYSSVGDWHSSSPDGMETKGRDTRFGGIKQECGIHTNN

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=225, Percent_Identity=33.3333333333333, Blast_Score=154, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325425, Length=250, Percent_Identity=31.2, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 28080; Mature: 28080

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKIHKNIQTNLMKYNQELVNMKPQEILTWGYEKFDDQFAITTSFGIQSSVLLHMVSKL
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHCCCEEEEEECCCHHHHHHHHHHHH
SLQKKIKIFWIDTGYLPTETYHYAENLIDRLSLKVEVLQSELSPARMEAKYGKLWETNKV
HHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
SDLDKYHELRKIKPLENGLEKYSIYCWASGVRAGQTETRNKMKFIDVIRQRLSLRPLLNW
HHHHHHHHHHHCCHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TNKDIFYYMEENNLPAHPLFIKGYSSVGDWHSSSPDGMETKGRDTRFGGIKQECGIHTNN
CCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MIEKIHKNIQTNLMKYNQELVNMKPQEILTWGYEKFDDQFAITTSFGIQSSVLLHMVSKL
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHCCCEEEEEECCCHHHHHHHHHHHH
SLQKKIKIFWIDTGYLPTETYHYAENLIDRLSLKVEVLQSELSPARMEAKYGKLWETNKV
HHHCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
SDLDKYHELRKIKPLENGLEKYSIYCWASGVRAGQTETRNKMKFIDVIRQRLSLRPLLNW
HHHHHHHHHHHCCHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
TNKDIFYYMEENNLPAHPLFIKGYSSVGDWHSSSPDGMETKGRDTRFGGIKQECGIHTNN
CCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1463852 [H]