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The map label for this gene is def [H]

Identifier: 126695417

GI number: 126695417

Start: 81806

End: 82411

Strand: Reverse

Name: def [H]

Synonym: P9301_00791

Alternate gene names: 126695417

Gene position: 82411-81806 (Counterclockwise)

Preceding gene: 126695419

Following gene: 126695416

Centisome position: 5.02

GC content: 33.5

Gene sequence:

>606_bases
GTGGCAAACCATTTTTCACAACTTGCAAAAAAGTCAAGAACAAATGGAAACGCAGAAAAAATTGCAAAAGAACAACCAGG
TAAGCCGTCTCTAGACATTTATAAACTTGGTGATGATGTATTAAGACAAAATTCCAAAAGAATAACTAAAGTTGACGAAT
CGATTAGAAAACTTGCTAGAGAAATGCTTCAAAGCATGTATGCAGCTAAAGGAATTGGACTTGCTGCACCTCAAATTGGA
ATCAACAAAGAGCTTCTTGTCATAGACGTAAATTTTGAAGATTCAGCAGCAGAACCTTTAATATTAATCAATCCAGAAAT
TACAGACTTTGGAACAACCCTTAATTCATATGAAGAAGGCTGCTTGAGTATACCTGGCGTCTATTTGAATGTAGTAAGAC
CATCAACTATAAAATTAAAATTTAGAGATGAAATGGGACGGCCACGTAAAATGAAAGCAGATGGACTTTTAGCGAGGTGT
ATTCAACACGAAATGGATCACTTAAACGGAATATTATTTGTAGATAGAGTTACATCAAAAGATGATTTGAACAAAGAACT
TTTAAAAGAAGGATTTAACGAAAAAGACGTTATCTCAATTAATTAA

Upstream 100 bases:

>100_bases
AATTGATCATCATTACTCATTTAAAAAAACTATAAAGAATGCAAATTAAAGTGCTAGTATTTTTATAGCTAAACTCTTAA
GTAAAAACCTTTTTTTTAAC

Downstream 100 bases:

>100_bases
TTTAATGACTGAAACAACAATATTTCAAAAAATCATTAATGAAGAAATACCCTGCGATAAGCTTTATGAAGATGAGTTTT
GTATTGCGTTTAATGATATC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MANHFSQLAKKSRTNGNAEKIAKEQPGKPSLDIYKLGDDVLRQNSKRITKVDESIRKLAREMLQSMYAAKGIGLAAPQIG
INKELLVIDVNFEDSAAEPLILINPEITDFGTTLNSYEEGCLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARC
IQHEMDHLNGILFVDRVTSKDDLNKELLKEGFNEKDVISIN

Sequences:

>Translated_201_residues
MANHFSQLAKKSRTNGNAEKIAKEQPGKPSLDIYKLGDDVLRQNSKRITKVDESIRKLAREMLQSMYAAKGIGLAAPQIG
INKELLVIDVNFEDSAAEPLILINPEITDFGTTLNSYEEGCLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARC
IQHEMDHLNGILFVDRVTSKDDLNKELLKEGFNEKDVISIN
>Mature_200_residues
ANHFSQLAKKSRTNGNAEKIAKEQPGKPSLDIYKLGDDVLRQNSKRITKVDESIRKLAREMLQSMYAAKGIGLAAPQIGI
NKELLVIDVNFEDSAAEPLILINPEITDFGTTLNSYEEGCLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARCI
QHEMDHLNGILFVDRVTSKDDLNKELLKEGFNEKDVISIN

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=181, Percent_Identity=27.6243093922652, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=43.6241610738255, Blast_Score=119, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=30.1204819277108, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24645726, Length=168, Percent_Identity=29.7619047619048, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 22512; Mature: 22381

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANHFSQLAKKSRTNGNAEKIAKEQPGKPSLDIYKLGDDVLRQNSKRITKVDESIRKLAR
CCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHH
EMLQSMYAAKGIGLAAPQIGINKELLVIDVNFEDSAAEPLILINPEITDFGTTLNSYEEG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCHHHCHHHHHHCC
CLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARCIQHEMDHLNGILFVDRVTSK
CCCCCCEEEEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
DDLNKELLKEGFNEKDVISIN
CCCCHHHHHCCCCCCCEEEEC
>Mature Secondary Structure 
ANHFSQLAKKSRTNGNAEKIAKEQPGKPSLDIYKLGDDVLRQNSKRITKVDESIRKLAR
CHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEECCHHHHHCCCHHHHHHHHHHHHHHH
EMLQSMYAAKGIGLAAPQIGINKELLVIDVNFEDSAAEPLILINPEITDFGTTLNSYEEG
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCHHHCHHHHHHCC
CLSIPGVYLNVVRPSTIKLKFRDEMGRPRKMKADGLLARCIQHEMDHLNGILFVDRVTSK
CCCCCCEEEEEECCCEEEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCC
DDLNKELLKEGFNEKDVISIN
CCCCHHHHHCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA