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The map label for this gene is dus2 [H]

Identifier: 126695351

GI number: 126695351

Start: 16721

End: 17725

Strand: Reverse

Name: dus2 [H]

Synonym: P9301_00131

Alternate gene names: 126695351

Gene position: 17725-16721 (Counterclockwise)

Preceding gene: 126695357

Following gene: 126695344

Centisome position: 1.08

GC content: 30.85

Gene sequence:

>1005_bases
ATGAATTTCATTCAGCCTAATTCTATTAAAAATATTCACAAATTAAGTATTGCTCCAATGATGGATTGTACTGATAAGCA
CTTCAGAATGATAATGAGAAAAATAAGTTCTGAAGCTCTATTGTATACGGAAATGATTGTGGCCCAGAGTTTAGTTTATA
CGGATAAAAAAGAATATTTTTTAGACTTTAATAGTGAAGAACACCCGATATCGATTCAGTTTGGTGGGGACGACCCTAAA
ATCCTCAAAGAAGCAGCCCAAATGGCACAGGATTGGGGTTATGACGAAATAAACTTTAATGTTGGTTGTCCCAGTCCTAG
GGTTTGTTCTGGAAATTTTGGCGCTTCTCTTATGAAAGATCCTGAAAAAGTAGCAAAATGTATAGAATCTTTAAAAAATA
ATTGCAACTTACCGGTTACGATCAAACACAGAATCGGTGTAGATAATGATGATAGTTTCGTTAACTTGAATAATTTCGTA
AGAATTATCGCAAATGCTGGTGCGGACAGATTTACAGTTCATGCAAGAAAAGCAATATTAAAAGGTCTAAATCCAAAACA
AAACAGGACCATACCTCCACTAAATTATGATGTAGTTAAAAAATTAAAAAAATCAAATCCAGAATTGTTAATAGAAATCA
ATGGGGGTTTAACAAATATCGATCAATCATTAAAAGCCTTAAATGATTTTGATGGGGTAATGATTGGACGGTCAATTTAT
AAACATCCCTTAAGATGGTCTGAAATTGATCAAAAAATTTATGGAATTAATAAAAAAAACAAATCTGCTTCTAATATTAT
ATTCTCCTTAATTCCATACATAGAAGCTCATATAAGTAATGGAGGAAAATCTTGGGATATTTGTAAACATCTTATAAATT
TAGTCGAAGGTATACCAAAAGCAAAAATTTGGAGAAATCAAATTTCAAAAAAATCTATAAAAAAGGAATTAGATATTGAA
TATCTATTTAAATTAACGTCAGCCCTTCAAGAAATGGGTTTCTAA

Upstream 100 bases:

>100_bases
CATGGGCTTAAAGATTATTTTTAAGAATGACATAGTAGGAATTAGAATAAAAGTTCTTCTTGATAGAAATTGATTCATAT
ATTTAAATTACAAAAAAGCA

Downstream 100 bases:

>100_bases
TCTGCCTCGTTTGAAGCATTGCTCTCATTGGAGACTCCCCTTTTTCTCCTACTCCTACCGCTTTCATATTCAGAATTTTC
AGAAGAATTTCCATAACTTC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MNFIQPNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVYTDKKEYFLDFNSEEHPISIQFGGDDPK
ILKEAAQMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKNNCNLPVTIKHRIGVDNDDSFVNLNNFV
RIIANAGADRFTVHARKAILKGLNPKQNRTIPPLNYDVVKKLKKSNPELLIEINGGLTNIDQSLKALNDFDGVMIGRSIY
KHPLRWSEIDQKIYGINKKNKSASNIIFSLIPYIEAHISNGGKSWDICKHLINLVEGIPKAKIWRNQISKKSIKKELDIE
YLFKLTSALQEMGF

Sequences:

>Translated_334_residues
MNFIQPNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVYTDKKEYFLDFNSEEHPISIQFGGDDPK
ILKEAAQMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKNNCNLPVTIKHRIGVDNDDSFVNLNNFV
RIIANAGADRFTVHARKAILKGLNPKQNRTIPPLNYDVVKKLKKSNPELLIEINGGLTNIDQSLKALNDFDGVMIGRSIY
KHPLRWSEIDQKIYGINKKNKSASNIIFSLIPYIEAHISNGGKSWDICKHLINLVEGIPKAKIWRNQISKKSIKKELDIE
YLFKLTSALQEMGF
>Mature_334_residues
MNFIQPNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVYTDKKEYFLDFNSEEHPISIQFGGDDPK
ILKEAAQMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKDPEKVAKCIESLKNNCNLPVTIKHRIGVDNDDSFVNLNNFV
RIIANAGADRFTVHARKAILKGLNPKQNRTIPPLNYDVVKKLKKSNPELLIEINGGLTNIDQSLKALNDFDGVMIGRSIY
KHPLRWSEIDQKIYGINKKNKSASNIIFSLIPYIEAHISNGGKSWDICKHLINLVEGIPKAKIWRNQISKKSIKKELDIE
YLFKLTSALQEMGF

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=232, Percent_Identity=27.5862068965517, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI40807366, Length=169, Percent_Identity=29.585798816568, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI145693211, Length=318, Percent_Identity=40.251572327044, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1789660, Length=241, Percent_Identity=26.5560165975104, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=24.896265560166, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=26.3473053892216, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25144369, Length=226, Percent_Identity=26.5486725663717, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=206, Percent_Identity=30.0970873786408, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24580595, Length=226, Percent_Identity=29.2035398230088, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920448, Length=226, Percent_Identity=29.2035398230088, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37902; Mature: 37902

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIQPNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVYTDKKEYF
CCCCCCCCCCHHHHHHCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHEE
LDFNSEEHPISIQFGGDDPKILKEAAQMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKD
EECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCHHHCC
PEKVAKCIESLKNNCNLPVTIKHRIGVDNDDSFVNLNNFVRIIANAGADRFTVHARKAIL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHH
KGLNPKQNRTIPPLNYDVVKKLKKSNPELLIEINGGLTNIDQSLKALNDFDGVMIGRSIY
CCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHH
KHPLRWSEIDQKIYGINKKNKSASNIIFSLIPYIEAHISNGGKSWDICKHLINLVEGIPK
HCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
AKIWRNQISKKSIKKELDIEYLFKLTSALQEMGF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFIQPNSIKNIHKLSIAPMMDCTDKHFRMIMRKISSEALLYTEMIVAQSLVYTDKKEYF
CCCCCCCCCCHHHHHHCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHEE
LDFNSEEHPISIQFGGDDPKILKEAAQMAQDWGYDEINFNVGCPSPRVCSGNFGASLMKD
EECCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEECCCCCCHHHCC
PEKVAKCIESLKNNCNLPVTIKHRIGVDNDDSFVNLNNFVRIIANAGADRFTVHARKAIL
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHH
KGLNPKQNRTIPPLNYDVVKKLKKSNPELLIEINGGLTNIDQSLKALNDFDGVMIGRSIY
CCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHH
KHPLRWSEIDQKIYGINKKNKSASNIIFSLIPYIEAHISNGGKSWDICKHLINLVEGIPK
HCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
AKIWRNQISKKSIKKELDIEYLFKLTSALQEMGF
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]