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The map label for this gene is pyrB [H]

Identifier: 126455367

GI number: 126455367

Start: 3067588

End: 3068673

Strand: Reverse

Name: pyrB [H]

Synonym: BURPS1106A_3145

Alternate gene names: 126455367

Gene position: 3068673-3067588 (Counterclockwise)

Preceding gene: 126452664

Following gene: 126452005

Centisome position: 76.94

GC content: 66.11

Gene sequence:

>1086_bases
GTGCGCGTCGCGCATCCGGGCGCCGTTTGGCAACACGTTGGAATCACGCACATCATGACGACCGACACCTCCGGCCGCAC
CGGCAATCCCGCCGCGGCCGCGCCCGCCGAGCGCTTTCGCTATGGCTTTCTGAAGGGCAACCCGCAGCTCACGAAGAACG
GCGAGCTCAAGCATCTGTTGACGATCGAGGGCCTGCCGCGCGCGATCCTCAATCAGATTCTCGACACCGCCGAGCAGTTC
GTCAGCGTGACCGACCGCGAAGTGAAGAAGGTGCCGCTCTTGCGCGGCAAATCGGTATTCAACCTGTTCTTCGAGAATTC
GACGCGCACGCGCACGACGTTCGAGATCGCCGCGAAGCGGCTGTCCGCCGACGTGATCAACCTGAACATCAACGCGTCGT
CGACGAGCAAGGGCGAGTCGCTCCTCGACACGATCAACAATCTGTCGGCGATGCACGCCGATCTCTTCGTCGTGCGCCAC
GCATCGAGCGGCGCGCCGTACCTGATCGCCGAGCACTGCGCGCCGCACGTGCACGTGATCAACGCGGGCGACGGCCGTCA
CGCGCACCCGACGCAGGGCCTTCTCGACATGTACACGATCCGCCACTACAAGCGCGACTTCACGAAGCTGCGCGTCGCGA
TCGTCGGCGACATTCTCCATTCGCGCGTCGCGCGCTCGGACATTCACGCGCTGACGACGCTCGGCGTGCCCGAGGTGCGC
GCGATCGGCCCGCGCACGCTGCTGCCGGGCGGGCTCGAGCAGATGGGCGTGCGCGTGTTCCACAATCTCGACGAAGGCCT
GAGGGACGTCGACGTGATCATCATGCTGCGCCTGCAGAACGAGCGGATGAGCGGCGCGCTGCTGCCGTCCGCGCAGGAAT
ATTTCAAGAGCTGGGGCTTGACGCCCGAGCGCCTTGCGCTCGCCGCGCCCGACGCGATCGTGATGCACCCGGGGCCGATG
AACCGCGGCGTCGAGATCGATTCGCAAGTGGCCGACGGCCCGCAATCGGTGATCCTGAACCAGGTGACGTTCGGCATCGC
GGTGCGCATGGCCGTGATGGGCATCGTCGCGGGCACGAGCGACTGA

Upstream 100 bases:

>100_bases
CGGACGCGACGCTCGTGCTCGAGCGCGCGGACGCGGGACGCTTCACGCTTCGCACCGAGGCGCGCGCGGGCTGAACGCGC
GCGACGCATTCCGGCCGCCC

Downstream 100 bases:

>100_bases
CGGGGCGCGGGCATCGCGGGCAACGCGGCGGGCCCGCACCGCGCGCCGGCTTCGCCGAAATCAACGCATTCAACGCATCA
ACAGACAGCGCATGAAGATT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRVAHPGAVWQHVGITHIMTTDTSGRTGNPAAAAPAERFRYGFLKGNPQLTKNGELKHLLTIEGLPRAILNQILDTAEQF
VSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMHADLFVVRH
ASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVR
AIGPRTLLPGGLEQMGVRVFHNLDEGLRDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAAPDAIVMHPGPM
NRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGTSD

Sequences:

>Translated_361_residues
MRVAHPGAVWQHVGITHIMTTDTSGRTGNPAAAAPAERFRYGFLKGNPQLTKNGELKHLLTIEGLPRAILNQILDTAEQF
VSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMHADLFVVRH
ASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVR
AIGPRTLLPGGLEQMGVRVFHNLDEGLRDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAAPDAIVMHPGPM
NRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGTSD
>Mature_361_residues
MRVAHPGAVWQHVGITHIMTTDTSGRTGNPAAAAPAERFRYGFLKGNPQLTKNGELKHLLTIEGLPRAILNQILDTAEQF
VSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLNINASSTSKGESLLDTINNLSAMHADLFVVRH
ASSGAPYLIAEHCAPHVHVINAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVR
AIGPRTLLPGGLEQMGVRVFHNLDEGLRDVDVIIMLRLQNERMSGALLPSAQEYFKSWGLTPERLALAAPDAIVMHPGPM
NRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGTSD

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=33.974358974359, Blast_Score=159, Evalue=4e-39,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=33.6569579288026, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=31.8318318318318, Blast_Score=124, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=30.6709265175719, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24642586, Length=317, Percent_Identity=34.7003154574132, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 39379; Mature: 39379

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAHPGAVWQHVGITHIMTTDTSGRTGNPAAAAPAERFRYGFLKGNPQLTKNGELKHLL
CCCCCCCHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCEEEEE
TIEGLPRAILNQILDTAEQFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
LSADVINLNINASSTSKGESLLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHVI
HCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCEEEEE
NAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVR
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AIGPRTLLPGGLEQMGVRVFHNLDEGLRDVDVIIMLRLQNERMSGALLPSAQEYFKSWGL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCC
TPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGTS
CHHHEEEECCCEEEECCCCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
D
C
>Mature Secondary Structure
MRVAHPGAVWQHVGITHIMTTDTSGRTGNPAAAAPAERFRYGFLKGNPQLTKNGELKHLL
CCCCCCCHHHHHCCEEEEEEECCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCEEEEE
TIEGLPRAILNQILDTAEQFVSVTDREVKKVPLLRGKSVFNLFFENSTRTRTTFEIAAKR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHH
LSADVINLNINASSTSKGESLLDTINNLSAMHADLFVVRHASSGAPYLIAEHCAPHVHVI
HCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHEEEEEEECCCCCCEEECCCCCCEEEEE
NAGDGRHAHPTQGLLDMYTIRHYKRDFTKLRVAIVGDILHSRVARSDIHALTTLGVPEVR
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AIGPRTLLPGGLEQMGVRVFHNLDEGLRDVDVIIMLRLQNERMSGALLPSAQEYFKSWGL
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCC
TPERLALAAPDAIVMHPGPMNRGVEIDSQVADGPQSVILNQVTFGIAVRMAVMGIVAGTS
CHHHEEEECCCEEEECCCCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA