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The map label for this gene is rumA [H]

Identifier: 126455291

GI number: 126455291

Start: 2222042

End: 2223439

Strand: Reverse

Name: rumA [H]

Synonym: BURPS1106A_2233

Alternate gene names: 126455291

Gene position: 2223439-2222042 (Counterclockwise)

Preceding gene: 126453166

Following gene: 126452672

Centisome position: 55.75

GC content: 67.31

Gene sequence:

>1398_bases
GTGTCAGAAGCCGTCCCCCTCTCCACGCGCCGCGCATCGTCCGCGGGCGACGCGCCCGGTCGCGCCCCGGTTCTCGATAT
CGATTCGCTCGACATGGAGGCGCGCGGCGTCGGCCGCGTCGTCGACGAAAACGGCGAGCCCGGCAAGGTGATTTTCGTCG
AGGGCGCGTTGCCCGGCGAGCGCGTCACTTATTCGAGCTTTCGCCGCAAGCCGACCTACGAGCAGGCGCAGGTCGTCGAT
ATTCTGCGCCCGAGCGTGATGCGCACGCAGCCGAAATGCGCGTTTTTCGGCACGTGCGGCGGCTGCTCGATGCAGCATCT
CGACATGCGCGCGCAGGTCGCGATCAAGCAGCGCGTGCTCGAGGATAACCTCTGGCATCTCGCGAAGCTGCGCGCCGAGG
CGATGTTCGCGCCGATTCACGGGCCGTCGTGGGGCTATCGCTATCGTGCGCGCCTCACCGTGCGCCATGTCGAGAAGAAG
GGCGGCGTGCTCGTCGGCTTCCACGAGAAAAAGAGCAGCTACGTGGCCGACATGACGAGCTGCGAAGTGCTGCCGCCGCA
CGTGTCGGCGATGCTCGTGCCGCTGCGCCGGCTGGTCGAGCAATTATCGATCCGCGATCGCATGCCGCAGATCGAGCTCG
CGGTCGGCGCCCGGGTGACGGCGCTCGTGCTGCGCGTGCTCGAGCCGATCAACGCGGCCGACGAGGCGCTCCTGCGCGAA
TTCGCCGACACGCATCGCGTGCAGTTCTGGCTGCAGCCGAAAGGGCCGGATACGGTCGCGCCGTTCTATCCGCTTGACGC
GCAGCTCGATTACACGCTGCCGGAATTCGGGATCCGGATGCCGTTCAAGCCGACCGATTTCACGCAGGTCAATCACCAGA
TCAACCGCGTGCTCGTCGGCCGCGCGCTGCGGCTACTCGCGCCCGAACGCGGCGATCGCGTGCTCGATCTGTTCTGCGGG
ATCGGCAACTTCACGCTGCCGCTCGCGCGGCTCGCACGCGAAGTGGTCGGCATCGAGGGCAGCGACGCGCTGACTGCGCG
CGCGCTCGAGAATGCGAAGGAAAACGGCGTCGACGGCCATACGTCGTTCGCGTGCCGTAACCTGTTCGAGGTGACGGCGG
ACGATCTGCGCGCGCTCGGCGCATTCGACAAGTTCCTGGTCGATCCGCCGCGCGAAGGCGCACTCGCGGTATCGAAGGCG
CTCGCCGAGATCGCGCAGAGCGGCGCGGGGCCGTTGCCTGCGCGGATCGTGTACGTGTCGTGCAACCCGTCGACGCTCGC
GCGAGACGCGGGGCTCCTCGTGCACGAGGCGGGTTATCGGCTCAAGGGCGCGGGCGTCGTCAACATGTTCCCGCATACCT
CGCATGTCGAATCGATCGCGCTGTTCGAGCGCGATTGA

Upstream 100 bases:

>100_bases
ACGTTCCAACTGATGAGTCGCATCGTCGAATCTCCGTTGCTTGGCGGGCGTTCGTCTACAATCTCGCCTTTCCCCCACAA
TCGTTCGTCAGGAAAAGCTG

Downstream 100 bases:

>100_bases
CGCGGGAGCCGGGTGGCGCGTGCATGAAAAAAACCGGCCCGAAGGCCGGTTTTGGGGCAGGGGGCGCCGGCCTTCAGTTG
CGGTTGCCGCCGAAGATGCC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGERVTYSSFRRKPTYEQAQVVD
ILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHGPSWGYRYRARLTVRHVEKK
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVTALVLRVLEPINAADEALLRE
FADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPERGDRVLDLFCG
IGNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALGAFDKFLVDPPREGALAVSKA
LAEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD

Sequences:

>Translated_465_residues
MSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGERVTYSSFRRKPTYEQAQVVD
ILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHGPSWGYRYRARLTVRHVEKK
GGVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVTALVLRVLEPINAADEALLRE
FADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPERGDRVLDLFCG
IGNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALGAFDKFLVDPPREGALAVSKA
LAEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD
>Mature_464_residues
SEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGERVTYSSFRRKPTYEQAQVVDI
LRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVLEDNLWHLAKLRAEAMFAPIHGPSWGYRYRARLTVRHVEKKG
GVLVGFHEKKSSYVADMTSCEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVTALVLRVLEPINAADEALLREF
ADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVGRALRLLAPERGDRVLDLFCGI
GNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGHTSFACRNLFEVTADDLRALGAFDKFLVDPPREGALAVSKAL
AEIAQSGAGPLPARIVYVSCNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=200, Percent_Identity=31, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI51173878, Length=200, Percent_Identity=31, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789148, Length=446, Percent_Identity=36.7713004484305, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1787083, Length=399, Percent_Identity=25.062656641604, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1790403, Length=357, Percent_Identity=24.9299719887955, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24666579, Length=209, Percent_Identity=31.5789473684211, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 51088; Mature: 50957

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGE
CCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCC
RVTYSSFRRKPTYEQAQVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVL
CCCHHHHCCCCCHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDNLWHLAKLRAEAMFAPIHGPSWGYRYRARLTVRHVEKKGGVLVGFHEKKSSYVADMTS
HHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEEEEHHCCCCEEEEEECCCCCHHHHCCC
CEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVTALVLRVLEPINAADEALLRE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
FADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
HHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALRLLAPERGDRVLDLFCGIGNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGH
HHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TSFACRNLFEVTADDLRALGAFDKFLVDPPREGALAVSKALAEIAQSGAGPLPARIVYVS
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
CNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD
CCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHEEEEEECC
>Mature Secondary Structure 
SEAVPLSTRRASSAGDAPGRAPVLDIDSLDMEARGVGRVVDENGEPGKVIFVEGALPGE
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEECCCCCCCEEEEEECCCCCC
RVTYSSFRRKPTYEQAQVVDILRPSVMRTQPKCAFFGTCGGCSMQHLDMRAQVAIKQRVL
CCCHHHHCCCCCHHHHHHHHHHCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
EDNLWHLAKLRAEAMFAPIHGPSWGYRYRARLTVRHVEKKGGVLVGFHEKKSSYVADMTS
HHHHHHHHHHHHHHEEECCCCCCCCEEEEEEEEEEEHHCCCCEEEEEECCCCCHHHHCCC
CEVLPPHVSAMLVPLRRLVEQLSIRDRMPQIELAVGARVTALVLRVLEPINAADEALLRE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
FADTHRVQFWLQPKGPDTVAPFYPLDAQLDYTLPEFGIRMPFKPTDFTQVNHQINRVLVG
HHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHH
RALRLLAPERGDRVLDLFCGIGNFTLPLARLAREVVGIEGSDALTARALENAKENGVDGH
HHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
TSFACRNLFEVTADDLRALGAFDKFLVDPPREGALAVSKALAEIAQSGAGPLPARIVYVS
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEE
CNPSTLARDAGLLVHEAGYRLKGAGVVNMFPHTSHVESIALFERD
CCCHHHHHHCCEEEECCCCEEECCCEEEECCCCCCHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA