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The map label for this gene is citA [H]

Identifier: 126455057

GI number: 126455057

Start: 2913104

End: 2914399

Strand: Reverse

Name: citA [H]

Synonym: BURPS1106A_2968

Alternate gene names: 126455057

Gene position: 2914399-2913104 (Counterclockwise)

Preceding gene: 126454187

Following gene: 126453882

Centisome position: 73.07

GC content: 65.9

Gene sequence:

>1296_bases
ATGTCTACCGCGTCCCCCGCAATCCAGCAGGAATCCAAAGCCAGGACCGTGTTCCGCGTCGTCAGCGGCAACTTCCTGGA
GATGTACGACTTCATGGTCTACGGCTACTACGCGTCCGCGATCGCCAAGACCTACTTCCCGAGCGGCAACGCGTTCGCCT
CGCTGATGCTGTCGCTGTCGGTGTTCGGCGCGGGCTTTCTGATGCGTCCCGTCGGCGCGATCGTGCTCGGCGCGTACATC
GACCATCACGGCCGCCGCAAGGGCCTCATCCTGACGCTCGCGTTGATGGCGCTCGGCACGCTGACCGTCGCGACGATTCC
GGGCTACGCGACGATCGGCGTGCTCGCGCCGATTCTCGTGCTGCTCGGCCGCCTGCTGCAGGGTTTTTCGGCGGGCGTCG
AGCTGGGCGGCGTATCGGTGTATCTGTCCGAGATCGCGACGAAGGGCAACAAGGGCTTCTATACGTCGTGGCAGTCGGGC
AGCCAGCAGGTGGCCGTGGTGTTCGCCGCGTTCGTCGGCGTGCTGCTGAACCGCGCGCTGCCTGTCGAGCAAATGACGTC
GTGGGGCTGGCGCATCCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGCTCGCTGAAGGAGA
CCGACGAGTTCCTCGCGAAGCGCCGCCGCCCGAGCATGGGCGAGATCATGAAGTCGATGCTCGAGAACTGGGGCGTGGTG
CTGGCCGGCATGGGGATGGTCATCATGACGACCGTGTCGTTCTACATGATCACCGCGTACACGCCGACTTTCGGCAAGGA
AGTGCTGCATCTGTCGGCGATCGACGCGCTCGTCGTGACCGTTTGCGTCGGGCTGTCGAACCTGGTCTGGCTGCCGCTGT
CGGGCGCGCTGTCCGATCGGATCGGCCGCCGCCCGGTGCTGATCGCGTTCACCGCGCTCACGATCCTCACCGCGTATCCG
GCGATGCAGTGGCTCGTCGGTTCGCCGTCGTTCCTGCGGCTGTTGGGCGTCGAGCTGTGGCTGTCGTTCCTGTACGGCTC
GTACAACGGCGCGATGGTCGTCGCGCTGACCGAGGTGATGCCGGCCGACGTGCGCACCGCGGGCTTCTCGCTCGCTTACA
GCCTCGCGACGACGATCGGCGGCTTCACGCCGGCGATCTCGACGCTGCTGATTCACGAGACCGGCAACAAGGCGGCGCCG
GGCCTCTGGCTCGGCCTCGCCGCGATCTGCGGGCTGATCGCGACGCTCGTGCTGTACCGGTCGCCTGAAGCGCGCAATCA
ATACAAGACGACTTGA

Upstream 100 bases:

>100_bases
TCGGTCTGCGCGGGCCCCGGCGCGCCGGGGCCAGAAACACGATGTGACACGCGCCGTGCGCCGCCCCCGGCGGTCGCGGC
GCAGCCTTATCGAGAGAGAC

Downstream 100 bases:

>100_bases
GCGAACCGGCCGCACGCGGCCCAGCAGGCCGCCGTCGACGAATCGACAAACGGAGCGCGTCGCGCTCCGTTTGGGTTGGG
GCGAAGCGTCGGCGCGGCGC

Product: citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSG
SQQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRRRPSMGEIMKSMLENWGVV
LAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLVWLPLSGALSDRIGRRPVLIAFTALTILTAYP
AMQWLVGSPSFLRLLGVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETGNKAAP
GLWLGLAAICGLIATLVLYRSPEARNQYKTT

Sequences:

>Translated_431_residues
MSTASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYI
DHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSG
SQQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRRRPSMGEIMKSMLENWGVV
LAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLVWLPLSGALSDRIGRRPVLIAFTALTILTAYP
AMQWLVGSPSFLRLLGVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETGNKAAP
GLWLGLAAICGLIATLVLYRSPEARNQYKTT
>Mature_430_residues
STASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLSVFGAGFLMRPVGAIVLGAYID
HHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILVLLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSGS
QQVAVVFAAFVGVLLNRALPVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRRRPSMGEIMKSMLENWGVVL
AGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLVWLPLSGALSDRIGRRPVLIAFTALTILTAYPA
MQWLVGSPSFLRLLGVELWLSFLYGSYNGAMVVALTEVMPADVRTAGFSLAYSLATTIGGFTPAISTLLIHETGNKAAPG
LWLGLAAICGLIATLVLYRSPEARNQYKTT

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=31.1163895486936, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788942, Length=431, Percent_Identity=33.1786542923434, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=28.8782816229117, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1789941, Length=425, Percent_Identity=31.2941176470588, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87082231, Length=162, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46470; Mature: 46338

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCC
>Mature Secondary Structure 
STASPAIQQESKARTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNAFASLMLSLS
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VFGAGFLMRPVGAIVLGAYIDHHGRRKGLILTLALMALGTLTVATIPGYATIGVLAPILV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYTSWQSGSQQVAVVFAAFVGVLLNRAL
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCC
PVEQMTSWGWRIPFLIGCLIVPFLFLIRRSLKETDEFLAKRRRPSMGEIMKSMLENWGVV
CHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
LAGMGMVIMTTVSFYMITAYTPTFGKEVLHLSAIDALVVTVCVGLSNLVWLPLSGALSDR
HHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHH
IGRRPVLIAFTALTILTAYPAMQWLVGSPSFLRLLGVELWLSFLYGSYNGAMVVALTEVM
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHC
PADVRTAGFSLAYSLATTIGGFTPAISTLLIHETGNKAAPGLWLGLAAICGLIATLVLYR
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHC
SPEARNQYKTT
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]