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The map label for this gene is leuA [H]

Identifier: 126455016

GI number: 126455016

Start: 1258850

End: 1260397

Strand: Direct

Name: leuA [H]

Synonym: BURPS1106A_1286

Alternate gene names: 126455016

Gene position: 1258850-1260397 (Clockwise)

Preceding gene: 126452290

Following gene: 126453638

Centisome position: 31.56

GC content: 64.79

Gene sequence:

>1548_bases
ATGACAGACAAGCTGATCATTTTCGACACGACGTTGCGCGACGGCGAGCAATCGCCCGGTGCGTCGATGACGAAGGAAGA
GAAAATCCGTATCGCGAAGCAGCTCGAGCGGATGAAGGTCGACGTGATCGAAGCCGGCTTCGCGGCCAGCTCGAACGGCG
ATTTCGACGCGATCCAGACGATCGCGTCGCAAGTGAAGGACAGCACGATCTGCTCGCTCGCGCGCGCGAACGACAAGGAT
ATCCAGCGCGCGGCCGATGCGCTCAAGCCCGCGAACAGCTTCCGGATTCACACGTTCATCGCGACCTCGCCGCTGCACAT
GGAGAAAAAGCTGCGGATGACGCCGGACCAGGTGTTCGAGCAGGCGCGCCTCGCGGTGCGCTTCGCGCGCAAGTTCACCG
ACAACATCGAGTTCTCGCCGGAGGACGGCAGCCGCTCGGACATGGATTTCCTCTGCCGCGTGCTCGAGGCGGTGATCGCC
GAGGGCGCGACGACGATCAACATCGCGGACACCGTCGGCTACGGTGTGCCGGAGCTATACGGCAACCTCGTGAAGACGCT
GCGCGAGCGCATCCCGAACTCGGACAAGGCGATCTTCTCGGTGCACTGCCACAACGATCTGGGGATGGCGGTCGCGAACT
CGCTCGCCGGCGTGAAGATCGGCGGCGCGCGCCAGGTCGAATGCACGATCAACGGCCTCGGCGAGCGCGCGGGCAACACG
TCGCTCGAGGAAATCGTGATGGCCGTGAAGACCCGCAAGGATTACTTCGGCCTCGATCTCGGCATCGATACGACGCAGAT
CGTGCCGGCGTCGAAGCTCGTCTCGCAGATCACGGGTTTCGTCGTGCAGCCGAACAAGGCGGTCGTCGGCGCGAACGCGT
TCGCGCATGCGTCGGGCATTCACCAGGACGGCGTGCTGAAGGCGCGTGACACGTACGAGATCATGCGCGCAGAGGACGTG
GGCTGGACCGCGAACAAGATCGTGCTCGGCAAGCTGTCGGGCCGCAATGCATTCAAGCAGCGCCTGCAGGAGCTCGGCGT
GTCGCTCGACAGCGAGGCGGAGCTGAACGCCGCGTTCGCGCGCTTCAAGGATCTCGCGGATCGCAAGGCCGAGATCTTCG
ACGAGGACATCATCGCGATCGTCACCGAAGAGGAATCGGCGCTCGCGCAGGAGCACGAGCACTACAAGTTCGTGTCGCTC
GCGCAGCGCTCGGAGACGGGTGAGCGGCCGCAGGCGAAGGTCGTGTTCGCCGTCGACGGCGACGAGGTGGCGGGCGAGGC
GAGCGGCAACGGCCCTGTCGACGCGACGTTCAACGCGATCGAAACCGAAGTGGGCAGCGGCGCCGAACTGCTGCTGTACT
CGGTCAACGCGATCACGACGGGTACGCAGGCGCAGGGCGAGGTGACGGTGCGGCTGTCGAAGAGCGGGCGGATCGTCAAC
GGCGTCGGCACGGATCCGGACATCGTTGCCGCGTCGGCGAAAGCGTATATCGCCGCGCTCAACAAGCTGTACTCGAACGC
GGACAAGCTCAACCCGCAACGCGCGTAG

Upstream 100 bases:

>100_bases
CCGTTTTCGCCCTCCGTCGAGCTTCGTCTGTTGTCTAGCGTCGCCTGCGGTGGCGCGAGCTTCATCACGTGTGTTTCCCC
ATTCGACACCTGGAGTCCCC

Downstream 100 bases:

>100_bases
CGCCCCGCCGCGCAACTCGCGCGCTGAGCGGCATGGCCGCCGGGGCGCGCATGAACGACGCCCCGCGGGCTAGAGGCCTC
GCGGGGCGTTTTTGCTCGAC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIQTIASQVKDSTICSLARANDKD
IQRAADALKPANSFRIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDNIEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
SLEEIVMAVKTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFARFKDLADRKAEIFDEDIIAIVTEEESALAQEHEHYKFVSL
AQRSETGERPQAKVVFAVDGDEVAGEASGNGPVDATFNAIETEVGSGAELLLYSVNAITTGTQAQGEVTVRLSKSGRIVN
GVGTDPDIVAASAKAYIAALNKLYSNADKLNPQRA

Sequences:

>Translated_515_residues
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIQTIASQVKDSTICSLARANDKD
IQRAADALKPANSFRIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDNIEFSPEDGSRSDMDFLCRVLEAVIA
EGATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
SLEEIVMAVKTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDV
GWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFARFKDLADRKAEIFDEDIIAIVTEEESALAQEHEHYKFVSL
AQRSETGERPQAKVVFAVDGDEVAGEASGNGPVDATFNAIETEVGSGAELLLYSVNAITTGTQAQGEVTVRLSKSGRIVN
GVGTDPDIVAASAKAYIAALNKLYSNADKLNPQRA
>Mature_514_residues
TDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIQTIASQVKDSTICSLARANDKDI
QRAADALKPANSFRIHTFIATSPLHMEKKLRMTPDQVFEQARLAVRFARKFTDNIEFSPEDGSRSDMDFLCRVLEAVIAE
GATTINIADTVGYGVPELYGNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNTS
LEEIVMAVKTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVG
WTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFARFKDLADRKAEIFDEDIIAIVTEEESALAQEHEHYKFVSLA
QRSETGERPQAKVVFAVDGDEVAGEASGNGPVDATFNAIETEVGSGAELLLYSVNAITTGTQAQGEVTVRLSKSGRIVNG
VGTDPDIVAASAKAYIAALNKLYSNADKLNPQRA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=511, Percent_Identity=49.119373776908, Blast_Score=464, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6324682, Length=544, Percent_Identity=25, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324225, Length=559, Percent_Identity=25.9391771019678, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320019, Length=378, Percent_Identity=28.8359788359788, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6320071, Length=378, Percent_Identity=28.5714285714286, Blast_Score=122, Evalue=1e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 55586; Mature: 55455

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIQT
CCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IASQVKDSTICSLARANDKDIQRAADALKPANSFRIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHH
QARLAVRFARKFTDNIEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFA
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RFKDLADRKAEIFDEDIIAIVTEEESALAQEHEHYKFVSLAQRSETGERPQAKVVFAVDG
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
DEVAGEASGNGPVDATFNAIETEVGSGAELLLYSVNAITTGTQAQGEVTVRLSKSGRIVN
CCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCEECCCCCCCEEEEEEECCCCEEE
GVGTDPDIVAASAKAYIAALNKLYSNADKLNPQRA
CCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
TDKLIIFDTTLRDGEQSPGASMTKEEKIRIAKQLERMKVDVIEAGFAASSNGDFDAIQT
CCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IASQVKDSTICSLARANDKDIQRAADALKPANSFRIHTFIATSPLHMEKKLRMTPDQVFE
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHCCCHHHHHH
QARLAVRFARKFTDNIEFSPEDGSRSDMDFLCRVLEAVIAEGATTINIADTVGYGVPELY
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEHHHHCCCCHHHH
GNLVKTLRERIPNSDKAIFSVHCHNDLGMAVANSLAGVKIGGARQVECTINGLGERAGNT
HHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCEEECCCEEEEEEEECCCCCCCCC
SLEEIVMAVKTRKDYFGLDLGIDTTQIVPASKLVSQITGFVVQPNKAVVGANAFAHASGI
CHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHHHCEEECCCCEEECCCHHHHHCCC
HQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLQELGVSLDSEAELNAAFA
CCCCCEECCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHH
RFKDLADRKAEIFDEDIIAIVTEEESALAQEHEHYKFVSLAQRSETGERPQAKVVFAVDG
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
DEVAGEASGNGPVDATFNAIETEVGSGAELLLYSVNAITTGTQAQGEVTVRLSKSGRIVN
CCCCCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCEECCCCCCCEEEEEEECCCCEEE
GVGTDPDIVAASAKAYIAALNKLYSNADKLNPQRA
CCCCCCCHHHHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA