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The map label for this gene is dctD [H]

Identifier: 126454917

GI number: 126454917

Start: 445433

End: 446788

Strand: Direct

Name: dctD [H]

Synonym: BURPS1106A_0478

Alternate gene names: 126454917

Gene position: 445433-446788 (Clockwise)

Preceding gene: 126451792

Following gene: 126452595

Centisome position: 11.17

GC content: 69.76

Gene sequence:

>1356_bases
ATGGCGAACCGCCTGCAAGTGATCTACATCGAAGACGACGAGCTCGTGCGCCGCGCGAGCGTGCAAAGCCTGCAGCTCGC
GGGGTTCGACGTCGTCGGGTTCGGTTCGGTCGAGGCGGCCGAGAAGGCGATCGTCGGCGACGCGACCGGCGTGATCGTAT
CGGACATTCGCCTGCCCGGCGCGAGCGGCCTCGAGCTGCTCGCGCAATGCCGCGAGCGTACGCCCGACGTGCCCGTCGTG
CTCGTCACCGGGCACGGCGACATTTCGATGGCCGTGCAGGCGATGCGCGACGGCGCCTACGACTTCATCGAAAAGCCGTT
CGCCGCCGAGCGGCTGACCGAAACGGTGCGGCGCGCGCTGGAGCGGCGCGCGCTCGTGCTCGAGAATCACGCGCTGCGGC
GCGAACTCGCCGGGCAGGGCGTCGTCGCGCCGCGGATCATCGGCCGCAGCCCGGCGATCGAGCAGGTGCGGCGGCTGATC
GCGAATGTCGCGCCCACCGACGCGTCGGTGCTGATCAACGGCGACACCGGCGCCGGCAAGGAACTGATCGCGCGCAGCCT
GCACGAGTTGTCGCCGCGCCGCGACAAGCCGTTCATCGCGGTCAACTGCGGCGCGCTGCCGGAGCCGATGTTCGAATCCG
AGATGTTCGGCTACGAGCCGGGCGCGTTCACCGGCGCGGCGAAGCGCCGTATCGGCAAGCTCGAATATGCGTCAGGCGGC
ACGCTGTTCCTCGACGAGATCGAAAGCATGCCGCTTGCGCTGCAGGTCAAGCTGCTGCGCGTGCTGCAGGACGGCGTGCT
CGAGCGGCTCGGCTCGAATCAGCCGATTCGTGTGAATTGCCGCGTGGTCGCGGCGGCGAAGGGCGACATGAGCGAGCACG
TCGCGGCCGGCACGTTCCGGCGCGACCTGCTGTATCGGCTGAACGTCGTGACGATCGCGCTGCCGCCGCTCGCCGAGCGC
CGCGAGGACATCGTGCCGCTCTTCGAGCATTTCATGCTCGACGCGGCGGTTCGCTACGGGCGCCCCGCGCCGCTGCTCAC
CGATCGCCAGCGCGCGAGCCTGATGCAGCGCGACTGGCCGGGCAACGTGCGTGAGCTGCGCAACGCGGCCGACCGCTTCG
TGCTCGGCGTGACGGAGGGCATCGTCGGCGACGCGGGCCCGGAGACGGACGAGCACGCCGAGCAGTCGCTCAAGGAGCGC
GTCGAGCAATTCGAGCGCGCGGTGATTGCCGAGACGCTGAACCGCACGGGCGGCGCGGTCGCGACGACGGCCGACAAGCT
GCACGTCGGCAAGGCGACGCTCTACGAGAAGATGAAGCGCTACGGGCTGAGCGCGAAAGGAGAAACGGACCGCTGA

Upstream 100 bases:

>100_bases
GACGCTGCGCCGCGCGCGCACGCATCATCCGGACACTTCCGCCGCGAGTTGAGCGCGCACCTTTCGCGCGGCCCGCGTCA
GCATCATGGGGTGACAGAAG

Downstream 100 bases:

>100_bases
TGCACGGGCCGCTCGCCGAGGCGGGCGCGGCCCGCGTCGGGCGGTCCGTTCGACGGCGGCGTCGCGCGCGGCGTGCCGCG
CCGCGAGAAAGGGCGTGACT

Product: C4-dicarboxylate transport transcriptional response regulator DctD

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDATGVIVSDIRLPGASGLELLAQCRERTPDVPVV
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLTETVRRALERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWPGNVRELRNAADRFVLGVTEGIVGDAGPETDEHAEQSLKER
VEQFERAVIAETLNRTGGAVATTADKLHVGKATLYEKMKRYGLSAKGETDR

Sequences:

>Translated_451_residues
MANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDATGVIVSDIRLPGASGLELLAQCRERTPDVPVV
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLTETVRRALERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWPGNVRELRNAADRFVLGVTEGIVGDAGPETDEHAEQSLKER
VEQFERAVIAETLNRTGGAVATTADKLHVGKATLYEKMKRYGLSAKGETDR
>Mature_450_residues
ANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDATGVIVSDIRLPGASGLELLAQCRERTPDVPVVL
VTGHGDISMAVQAMRDGAYDFIEKPFAAERLTETVRRALERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLIA
NVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGGT
LFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFRRDLLYRLNVVTIALPPLAERR
EDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWPGNVRELRNAADRFVLGVTEGIVGDAGPETDEHAEQSLKERV
EQFERAVIAETLNRTGGAVATTADKLHVGKATLYEKMKRYGLSAKGETDR

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=38.034188034188, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=38.0530973451327, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790437, Length=441, Percent_Identity=37.18820861678, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1788905, Length=445, Percent_Identity=37.0786516853933, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI87082117, Length=348, Percent_Identity=41.6666666666667, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=40.625, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1789087, Length=341, Percent_Identity=43.108504398827, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=42.4148606811146, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1786524, Length=355, Percent_Identity=38.5915492957746, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=47.3913043478261, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=37.5384615384615, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=35.655737704918, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1790552, Length=119, Percent_Identity=26.890756302521, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49315; Mature: 49184

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDATGVIVSDIRLPG
CCCCEEEEEECCHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHCCCCCCEEEEEEECCC
ASGLELLAQCRERTPDVPVVLVTGHGDISMAVQAMRDGAYDFIEKPFAAERLTETVRRAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVTEGIVGDAGPETDEHAEQSLKERVEQFERAVIAETLNRTGGAV
CCHHHHHHHHHHEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ATTADKLHVGKATLYEKMKRYGLSAKGETDR
EECHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANRLQVIYIEDDELVRRASVQSLQLAGFDVVGFGSVEAAEKAIVGDATGVIVSDIRLPG
CCCEEEEEECCHHHHHHHHHHHHEECCCEEEECCCHHHHHHHHCCCCCCEEEEEEECCC
ASGLELLAQCRERTPDVPVVLVTGHGDISMAVQAMRDGAYDFIEKPFAAERLTETVRRAL
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRALVLENHALRRELAGQGVVAPRIIGRSPAIEQVRRLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRIGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCHHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSEHVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPLLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVTEGIVGDAGPETDEHAEQSLKERVEQFERAVIAETLNRTGGAV
CCHHHHHHHHHHEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
ATTADKLHVGKATLYEKMKRYGLSAKGETDR
EECHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]