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The map label for this gene is garL [H]

Identifier: 126454865

GI number: 126454865

Start: 163087

End: 163872

Strand: Direct

Name: garL [H]

Synonym: BURPS1106A_0175

Alternate gene names: 126454865

Gene position: 163087-163872 (Clockwise)

Preceding gene: 126451563

Following gene: 126453702

Centisome position: 4.09

GC content: 73.16

Gene sequence:

>786_bases
ATGAGCACGCTCACCCATTCCCTGAAAGCACGTTTGCGCGACGGCGACGAGCCGCTGTTCGGCCTATGGCTGACGCTCGC
GAGCGAGGCGGCGACCGAGGCGCTCGCGCACGCCGGTTTCGACTGGCTGTGCATCGACATGGAGCACGCGCCGAACGACA
GCCGCGACGTCGCCGCGCAACTGCGCGCGCTCGCGGCCGCGCATCTGCCGAGCGAGCCCGTCGTGCGGGTGCCGGCGCGC
GAGCCGTGGTTCGTCAAGCGGGCGCTCGACGCGGGTGCGCGCACGCTGATGTTCCCGGGCGTCGAGACGGCCGACGAGGC
CGCGCATGCGGTGCGGCTCACGCGCTTTCAGGCGCCCGATGCGCCGGACGGGCTGCGCGGCGTTGCGGGCATCGTGCGCG
CGGCCGCTTATGGGATGCGGCGCGACTACGTGCAGACGGCGAACGCGCAGATCGCGACGATCGTGCAGATCGAATCGGCG
CGCGGCGTCGACGAAGCCGAGCGGATCGCGGCGACGCCGGGCGTCGATTGCGTATTCGTCGGGCCCGCCGACCTGTCCGC
GAGCCTCGGGCATCTCGGTGACACGAAGCATCCGGACGTCGCGGCCGCGCTCGAGCACGTGCTCGCGGCCGGGCGGCGCG
CCGGCGTGCCGGTCGGCATCTTCGCCGCGGATACGGCCGGCGCGCGCCAGTCTCTCGAAGCCGGATTCCGCGTGGTCGCG
TTGTCCGCGGACGTCGTGTGGCTGCTGCGCGCGACGCGACAGGCGCTGCAGGAGGTGCGGGGATGA

Upstream 100 bases:

>100_bases
TCGCGCGCGCCTGTTACCGCGCAGCGCTCCGCCCGGCGGTCTCGCCGCCCCTCTTTCCGAATCGCAGTCCGACCGTGCGT
CGCACGGCAGGAGGCCGCCG

Downstream 100 bases:

>100_bases
GCGGGAAACGCCGCGGCGCGCCGCTCTCTACGCGCACGCCGCGTGTGCTGCGCGGCGCGGTAATCGGTTCGCTCGCCGCG
GCGGCGCTCGGTGCGGCGGC

Product: HpcH/HpaI aldolase/citrate lyase family protein

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSTLTHSLKARLRDGDEPLFGLWLTLASEAATEALAHAGFDWLCIDMEHAPNDSRDVAAQLRALAAAHLPSEPVVRVPAR
EPWFVKRALDAGARTLMFPGVETADEAAHAVRLTRFQAPDAPDGLRGVAGIVRAAAYGMRRDYVQTANAQIATIVQIESA
RGVDEAERIAATPGVDCVFVGPADLSASLGHLGDTKHPDVAAALEHVLAAGRRAGVPVGIFAADTAGARQSLEAGFRVVA
LSADVVWLLRATRQALQEVRG

Sequences:

>Translated_261_residues
MSTLTHSLKARLRDGDEPLFGLWLTLASEAATEALAHAGFDWLCIDMEHAPNDSRDVAAQLRALAAAHLPSEPVVRVPAR
EPWFVKRALDAGARTLMFPGVETADEAAHAVRLTRFQAPDAPDGLRGVAGIVRAAAYGMRRDYVQTANAQIATIVQIESA
RGVDEAERIAATPGVDCVFVGPADLSASLGHLGDTKHPDVAAALEHVLAAGRRAGVPVGIFAADTAGARQSLEAGFRVVA
LSADVVWLLRATRQALQEVRG
>Mature_260_residues
STLTHSLKARLRDGDEPLFGLWLTLASEAATEALAHAGFDWLCIDMEHAPNDSRDVAAQLRALAAAHLPSEPVVRVPARE
PWFVKRALDAGARTLMFPGVETADEAAHAVRLTRFQAPDAPDGLRGVAGIVRAAAYGMRRDYVQTANAQIATIVQIESAR
GVDEAERIAATPGVDCVFVGPADLSASLGHLGDTKHPDVAAALEHVLAAGRRAGVPVGIFAADTAGARQSLEAGFRVVAL
SADVVWLLRATRQALQEVRG

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=234, Percent_Identity=46.1538461538462, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788578, Length=255, Percent_Identity=39.6078431372549, Blast_Score=156, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27603; Mature: 27471

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLTHSLKARLRDGDEPLFGLWLTLASEAATEALAHAGFDWLCIDMEHAPNDSRDVAAQ
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
LRALAAAHLPSEPVVRVPAREPWFVKRALDAGARTLMFPGVETADEAAHAVRLTRFQAPD
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCC
APDGLRGVAGIVRAAAYGMRRDYVQTANAQIATIVQIESARGVDEAERIAATPGVDCVFV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCEEEE
GPADLSASLGHLGDTKHPDVAAALEHVLAAGRRAGVPVGIFAADTAGARQSLEAGFRVVA
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCEEEEE
LSADVVWLLRATRQALQEVRG
ECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STLTHSLKARLRDGDEPLFGLWLTLASEAATEALAHAGFDWLCIDMEHAPNDSRDVAAQ
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
LRALAAAHLPSEPVVRVPAREPWFVKRALDAGARTLMFPGVETADEAAHAVRLTRFQAPD
HHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCC
APDGLRGVAGIVRAAAYGMRRDYVQTANAQIATIVQIESARGVDEAERIAATPGVDCVFV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHCCCCCCEEEE
GPADLSASLGHLGDTKHPDVAAALEHVLAAGRRAGVPVGIFAADTAGARQSLEAGFRVVA
CCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCEEEEE
LSADVVWLLRATRQALQEVRG
ECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA