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The map label for this gene is rfbB [H]

Identifier: 126454814

GI number: 126454814

Start: 3062918

End: 3063979

Strand: Reverse

Name: rfbB [H]

Synonym: BURPS1106A_3141

Alternate gene names: 126454814

Gene position: 3063979-3062918 (Counterclockwise)

Preceding gene: 126452991

Following gene: 126451933

Centisome position: 76.82

GC content: 65.07

Gene sequence:

>1062_bases
ATGATCTTGGTAACGGGCGGCGCCGGTTTCATCGGCGCCAACTTCGTGCTTGACTGGCTGGCTCAATCCGACGAGGCGGT
GCTGAACGTCGATAAGCTGACGTACGCGGGCAACCTCGGCACGTTGAAGTCGTTGCAGGGGAATCCGAAGCATGTATTCG
CCCGCGTCGACATCTGCGATCGCGCCGCAATCGACGCGCTGCTGGCGCAGCACAAGCCGCGCGCGATCGTGCACTTCGCG
GCCGAGAGCCACGTCGACCGTTCGATTCACGGTCCGGCCGATTTCGTGCAGACGAACGTCGTCGGCACCTTCACGCTGCT
CGAAGCCGCGCGCCAATACTGGAGCGCGCTCGGCACCGACGCGAAGGCGGCGTTCCGCTTCCTGCACGTATCGACGGACG
AAGTGTTCGGCTCGCTGTCGCCGGCCGATCCGCAGTTCTCCGAAACCACGCCGTACGCGCCGAACAGCCCGTACTCGGCG
ACGAAGGCGGGCTCCGACCACCTCGTACGCGCGTATCACCACACGTACGGGCTGCCGGTGCTCACGACGAACTGCTCGAA
CAACTACGGCCCGTATCAGTTCCCCGAGAAGCTGATTCCGCTGATGATCGCGAACGCGCTCGGCGGCAAGCCGCTGCCCG
TCTACGGCGACGGCCAGAACGTGCGGGACTGGCTGTACGTCGGCGATCACTGCAGCGCGATTCGCGAGGTGCTCGCGCGC
GGCGTGCCGGGCGAGACGTACAACGTCGGCGGCTGGAACGAAAAGAAGAATCTCGATGTCGTGCACACGCTGTGCGATCT
GCTCGACGAGGCCCGGCCGAAGGCGGGCGGCTCGTACCGCGATCAGATCACGTACGTGACCGATCGCCCCGGCCACGATC
GCCGCTATGCGATCGATGCGCGCAAGCTCGAGCGCGAGCTCGGCTGGAAGCCGGCCGAGACGTTCGAGACCGGACTTGCG
AAGACCGTGCGCTGGTATCTCGACCATCAGGAGTGGGTCGACGAAGTGGTGTCGGGCGATTACCGCAAGTGGGTCGAAAC
GAACTACGCGCAACGCGCGTAA

Upstream 100 bases:

>100_bases
GCGGTGTCATTCGAGCGACCAACGGCATGCTTCCGAGCGTGCCGGCCATGCTGCGCACGCCGGTTGTTTGACCGACTCCC
AATTTCAGCAAAGGAATCCC

Downstream 100 bases:

>100_bases
AGGTTGAACCGATGGCGCGCAAGGGCATTATTCTGGCCGGCGGTTCCGGCACGCGGCTGTATCCGATCACGCATGCGGTG
TCGAAGCAGCTGTTGCCGGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVLDWLAQSDEAVLNVDKLTYAGNLGTLKSLQGNPKHVFARVDICDRAAIDALLAQHKPRAIVHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWSALGTDAKAAFRFLHVSTDEVFGSLSPADPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALGGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLDVVHTLCDLLDEARPKAGGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVRWYLDHQEWVDEVVSGDYRKWVETNYAQRA

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVLDWLAQSDEAVLNVDKLTYAGNLGTLKSLQGNPKHVFARVDICDRAAIDALLAQHKPRAIVHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWSALGTDAKAAFRFLHVSTDEVFGSLSPADPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALGGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLDVVHTLCDLLDEARPKAGGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVRWYLDHQEWVDEVVSGDYRKWVETNYAQRA
>Mature_353_residues
MILVTGGAGFIGANFVLDWLAQSDEAVLNVDKLTYAGNLGTLKSLQGNPKHVFARVDICDRAAIDALLAQHKPRAIVHFA
AESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWSALGTDAKAAFRFLHVSTDEVFGSLSPADPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALGGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLDVVHTLCDLLDEARPKAGGSYRDQITYVTDRPGHDRRYAIDARKLERELGWKPAETFETGLA
KTVRWYLDHQEWVDEVVSGDYRKWVETNYAQRA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=34.3465045592705, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI42516563, Length=336, Percent_Identity=24.1071428571429, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI56237023, Length=359, Percent_Identity=26.4623955431755, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI56118217, Length=359, Percent_Identity=26.4623955431755, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI189083684, Length=359, Percent_Identity=26.4623955431755, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI4504031, Length=342, Percent_Identity=24.5614035087719, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=62.116991643454, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=61.6279069767442, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1786974, Length=357, Percent_Identity=27.1708683473389, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788366, Length=309, Percent_Identity=26.2135922330097, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=34.6385542168675, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=32.4483775811209, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=27.4011299435028, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982038, Length=356, Percent_Identity=27.247191011236, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=22.6586102719033, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=333, Percent_Identity=23.7237237237237, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=336, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=336, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=336, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=336, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=336, Percent_Identity=23.8095238095238, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=360, Percent_Identity=25.5555555555556, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19923002, Length=360, Percent_Identity=29.1666666666667, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=25.0755287009063, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=27.1641791044776, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39136; Mature: 39136

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLAQSDEAVLNVDKLTYAGNLGTLKSLQGNPKHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHEEECCCCCCHHHCCCCCCEEEEEECHHH
RAAIDALLAQHKPRAIVHFAAESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWSALGTD
HHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AKAAFRFLHVSTDEVFGSLSPADPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHHHEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALGGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLDVVHTLCDLLDEARPKAGGSYRDQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVRWYLDHQEWVDEVVSGDYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHCCHHCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLAQSDEAVLNVDKLTYAGNLGTLKSLQGNPKHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHEEECCCCCCHHHCCCCCCEEEEEECHHH
RAAIDALLAQHKPRAIVHFAAESHVDRSIHGPADFVQTNVVGTFTLLEAARQYWSALGTD
HHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AKAAFRFLHVSTDEVFGSLSPADPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPV
HHHHHHEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALGGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLDVVHTLCDLLDEARPKAGGSYRDQITYVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGLAKTVRWYLDHQEWVDEVVSGDYRKWVETNYAQRA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]