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The map label for this gene is purB [H]

Identifier: 126454575

GI number: 126454575

Start: 3347206

End: 3348594

Strand: Direct

Name: purB [H]

Synonym: BURPS1106A_3436

Alternate gene names: 126454575

Gene position: 3347206-3348594 (Clockwise)

Preceding gene: 126454791

Following gene: 126455076

Centisome position: 83.92

GC content: 66.38

Gene sequence:

>1389_bases
ATGTCCGACACCCGTCCCGATACCCTTTTCGCCCTCACCGCGCTCTCGCCGCTCGACGGCCGCTACGCCAGCAAGACCGA
GGCGCTGCGCGACTGGCTCTCCGAAGCCGCCTTCATGCGCCACCGCGTCACCGTCGAGGTGCACTGGCTGATCGCGCTGT
CGCGCGCCGGCTTCGCCGAAGTGCCGCGCTTCTCGGAAGCCGCCGAGCAGTTCCTGCTGCAGCTCGTCGAGCGCTTCACC
GCGCACGACGCCGCGCGCATCAAGGAAATCGAGCGCGTGACGAACCACGACGTGAAAGCCGTCGAGTACTGGCTGAAGGA
ATCCGTCAAGGGCCAGGTCGAGCTCGAGCAGGCGAGCGAATTCATCCACTTCGCATGCACGTCGGAAGACATCAACAACA
CGTCGCACGGCATGATGCTCGCCGGCGCGCGCCAGCACGTGATCCTGCCCGCGCTGCGCACGGTGCACGGGCGCCTCGTC
GCGCTCGCGCACGCGCACGCCGCGCAGCCGATGCTCTCGCGCACGCACGGCCAGCCGGCCAGCCCGACGACGCTCGGCAA
GGAAATCGCGAACGTGGCCGCGCGGCTCGCGCGCGCGATCGAACGCATCGAGAAGGTCGAGATCCTCGGCAAGATGAACG
GCGCGGTCGGCAACTTCAACGCGCATCTTTCCGCGTATCCGGAGTTCGACTGGGAAGCGTTCTCGCGCGACGTGATCGAG
CACCGCCTGAAGCTCACCTTCAACCCGTACACGATCCAGATCGAGCCGCACGACTACATGGCCGAGCTGTTCGACGCCGT
CTCGCGCGCGAACACGATCCTGCTCGACCTCGACCGCGACGTGTGGGGCTACATCTCGCTCGGCTACTTCAAGCAGAAGA
CGAAGGCGGGCGAAATCGGCTCGTCGACGATGCCGCACAAGGTCAACCCGATCGACTTCGAGAATTCGGAAGGCAATCTG
GGCCTCGCGAACGCGACGCTGCGCCACCTCGCCGACAAGTTGCCCGTGTCGCGCTGGCAGCGCGACCTCACCGATTCGAC
GGTGCTGCGCAACATCGGCGTCGCGTTCGGCTATTCGCTGCTCGCGTACGACGCGCTGATCCGCGGCCTCGACAAGCTCG
AAGTCAATCCGCAGCGCCTGAACGACGACCTCGACAATTGCTGGGAAGTGCTCGCCGAGCCGGTGCAGACGGTGATGCGC
CGCTACGGCATCGAGAACCCGTACGAGCAGTTGAAGGAGCTCACGCGCGGCAAGGGCATCACGCGCGACGCGCTGCAGCA
GTTCATCGGCACGCTCGCGATTGCCGAGGACGCGAAGGCGCGCCTGCTCGCGATGACGCCCGCGTCGTACATCGGCAAGG
CGGTCGAGCTCGCGAAGCGCATCGCGTAA

Upstream 100 bases:

>100_bases
CGGCGACGGCGCGCGGCGGCCGGGCGGCCGCGCGCCGATGGTAAAATTCGCGATTCTCCCCTTCACGTTTGAACCGCGGC
CGCGAGCCTCCAGCCCGATC

Downstream 100 bases:

>100_bases
CGTATCACGCGCGTCGGCGACGCGATGTGAAAAAGGCGCCCCGCGGTTCGCGGGGCGCCTTTCTTTTTGCGGCCGCTTCG
CGGCGCCCGGCGGCAGCGGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAEVPRFSEAAEQFLLQLVERFT
AHDAARIKEIERVTNHDVKAVEYWLKESVKGQVELEQASEFIHFACTSEDINNTSHGMMLAGARQHVILPALRTVHGRLV
ALAHAHAAQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE
HRLKLTFNPYTIQIEPHDYMAELFDAVSRANTILLDLDRDVWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNL
GLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDALIRGLDKLEVNPQRLNDDLDNCWEVLAEPVQTVMR
RYGIENPYEQLKELTRGKGITRDALQQFIGTLAIAEDAKARLLAMTPASYIGKAVELAKRIA

Sequences:

>Translated_462_residues
MSDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAEVPRFSEAAEQFLLQLVERFT
AHDAARIKEIERVTNHDVKAVEYWLKESVKGQVELEQASEFIHFACTSEDINNTSHGMMLAGARQHVILPALRTVHGRLV
ALAHAHAAQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE
HRLKLTFNPYTIQIEPHDYMAELFDAVSRANTILLDLDRDVWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNL
GLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDALIRGLDKLEVNPQRLNDDLDNCWEVLAEPVQTVMR
RYGIENPYEQLKELTRGKGITRDALQQFIGTLAIAEDAKARLLAMTPASYIGKAVELAKRIA
>Mature_461_residues
SDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAEVPRFSEAAEQFLLQLVERFTA
HDAARIKEIERVTNHDVKAVEYWLKESVKGQVELEQASEFIHFACTSEDINNTSHGMMLAGARQHVILPALRTVHGRLVA
LAHAHAAQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIEH
RLKLTFNPYTIQIEPHDYMAELFDAVSRANTILLDLDRDVWGYISLGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLG
LANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYDALIRGLDKLEVNPQRLNDDLDNCWEVLAEPVQTVMRR
YGIENPYEQLKELTRGKGITRDALQQFIGTLAIAEDAKARLLAMTPASYIGKAVELAKRIA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=62.5550660792952, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51763; Mature: 51631

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCCCC
VPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQVELEQASE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHH
FIHFACTSEDINNTSHGMMLAGARQHVILPALRTVHGRLVALAHAHAAQPMLSRTHGQPA
HHEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
SPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHH
HRLKLTFNPYTIQIEPHDYMAELFDAVSRANTILLDLDRDVWGYISLGYFKQKTKAGEIG
HHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAYDALIRGLDKLEVNPQRLNDDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGI
HHHHHHHHCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
TRDALQQFIGTLAIAEDAKARLLAMTPASYIGKAVELAKRIA
CHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDTRPDTLFALTALSPLDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEHHHCCCCCC
VPRFSEAAEQFLLQLVERFTAHDAARIKEIERVTNHDVKAVEYWLKESVKGQVELEQASE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEHHHHHH
FIHFACTSEDINNTSHGMMLAGARQHVILPALRTVHGRLVALAHAHAAQPMLSRTHGQPA
HHEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCC
SPTTLGKEIANVAARLARAIERIEKVEILGKMNGAVGNFNAHLSAYPEFDWEAFSRDVIE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHH
HRLKLTFNPYTIQIEPHDYMAELFDAVSRANTILLDLDRDVWGYISLGYFKQKTKAGEIG
HHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCCCCC
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNIGVAFGYSL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LAYDALIRGLDKLEVNPQRLNDDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGI
HHHHHHHHCHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
TRDALQQFIGTLAIAEDAKARLLAMTPASYIGKAVELAKRIA
CHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]