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The map label for this gene is oprB [H]

Identifier: 126454516

GI number: 126454516

Start: 843037

End: 844581

Strand: Direct

Name: oprB [H]

Synonym: BURPS1106A_0861

Alternate gene names: 126454516

Gene position: 843037-844581 (Clockwise)

Preceding gene: 126453664

Following gene: 126454129

Centisome position: 21.14

GC content: 71.39

Gene sequence:

>1545_bases
ATGAAGCGAAAACATGCTTTGACTGCACTCGCAGTCGCGCTGCTCGCCGCGGGCTGCACGCTCGCGCCGCGCTACGAGCG
TCCGGCCGCGCCGGTGTCGGGCGCGTTCCCCGCCGACGGCGTCTACGCCGCGCAGCCGGGCGCCGCGCCCGGCGCGCGCA
GCGCGAACGGCCAGGCGGCCGTCGATATCGGCTGGCGCGAGTTCTTCGTCGATCCGCGCCTGCAGCGGCTGATCGAGATC
GCGCTGAAGAACAACCGCGACCTGCGCGTGTCGGTGCTCAACGTCGAGGCGTCGCGCGCGCAGTATCAGATCACGCGCGC
GGGGCTCTTCCCGACGTTGAGCGGCACCGGCACGGGCACGATCCAGCGCACGCCGGCCGGCGTGTCGATCACCGGCCAGC
CGCTCATCTCGCGGACCTACAACGTCGGCGTCTCCGCGTCGTGGGAGCTCGACCTGTTCGGCCGCGTGCAGAGCCTGAAG
GATCAGGCGCTCGCGCAATACTTCGCCACCGCGCAGGCGCGCAAGGCCGCGGAGATCTCGCTCGTCGCGAGCGTCGCCGA
TCAGTACCTGACGCTGCTGTCGACCGACGATCTGCTGCAGGTCACGCAGAACACGCTGAAGTCGGCGCGCGCGTCCTACG
ATCTGACGAAGCTGCAGTTCGACAACGGCACCGGCTCGGAGCTCGACCTGCGCCAGGCGCAGACGGTGGTCGAGACCGCG
CTCGCGAGCCAGCAGGCGCAGGCGCGCGCCCGCGCGCAGGCGCTCAACGCGCTCGTGCTGCTGATCGGCGAGCCGCTGCC
CGACGATCTGCCGGCCGGCCTGCCGCTCAATGCGCAGAACCTGCTCACCGACATTCCGGCCGGGCTGCCGTCCGATCTGC
TCACGCGGCGCCCCGACATCATGCAGGCCGAGGAGACGCTGCGCGCGGCGAACGCGAACATCGGCGCGGCGCGCGCGGCG
TTCTTCCCGAAGATCTCGCTCACCAGCGCGTTCGGCACCGCGAGCCCGACGCTCGGCGGCCTGTTCAAGGCGGGCACGGC
GGCGTGGTCGTTCGCGCCGAACATCGCGCTGCCGATCTTCGAGGGTGGGCAGAACATCGCGAACCTCGATCTCGCGCACG
TGCAGAAGCGCATCGAGATCGCGAACTACGAGAAGGCGATCCAGAGCGCGTTTCGCGAGGTGTCGGACGGGCTTGCCGCG
CGCGGCACGTACGATCAGCAGATCGCGGCGCTCGAGCGCAACGAGCACGCGCAGCAGCGCCGCTACGATCTGTCGGACCT
GCGCTACAGGAACGGCGTCGACAGCTATCTGTCGGTGCTGACCGCGCAGACGGACCTGTATTCGGCGCAGCAGCAGTTGA
TCAGCGCGCGGCTCGCGCGCTGGACGAACCTCGTGGACCTGTATCGCGCGCTGGGCGGCGGGTGGCTCGAGCGCGCGGGC
GAGACGCCGCGCCCGGCGGACGCGCCCGTCGATTACGGCAAGGCGGCGCCCGCGCCGGCGTCGGCCGTGCCGGCTGCGTC
GGCCAGCGCGCCGGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
GAAGGAAGACGCCGACGAAGCGCTGCGCCTCGCGCACGAGCACATGCACCGTGACGACAAGCCGGAGCACGGCGACGACG
CTGGCAAGAAGGACTAACAG

Downstream 100 bases:

>100_bases
GCGGTTGACCGCGCCCGCGCGGCGGACGAAGGCAGCGGGTTCGTTCGTCGCGCGGTAAGCGATCGGCGGCGGCTTCGCCG
CATCGCGATGCACGAACAAA

Product: outer membrane efflux protein OprB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
ETPRPADAPVDYGKAAPAPASAVPAASASAPAAG

Sequences:

>Translated_514_residues
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
ETPRPADAPVDYGKAAPAPASAVPAASASAPAAG
>Mature_514_residues
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAAVDIGWREFFVDPRLQRLIEI
ALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGTIQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLK
DQALAQYFATAQARKAAEISLVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
LASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDIMQAEETLRAANANIGAARAA
FFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIFEGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAA
RGTYDQQIAALERNEHAQQRRYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
ETPRPADAPVDYGKAAPAPASAVPAASASAPAAG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=469, Percent_Identity=41.5778251599147, Blast_Score=327, Evalue=2e-90,
Organism=Escherichia coli, GI2367348, Length=480, Percent_Identity=24.5833333333333, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI87082199, Length=313, Percent_Identity=23.3226837060703, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54584; Mature: 54584

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
VDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGT
EECCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEEHHCCCCCCCCCCCCE
IQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEIS
EEECCCCCEEECCHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDI
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
MQAEETLRAANANIGAARAAFFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIF
HHHHHHHHHHCCCCCHHHHHHCCCEEHHHHCCCCCCCHHHHHHHCCHHHCCCCCCEEEEE
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHH
RYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ETPRPADAPVDYGKAAPAPASAVPAASASAPAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRKHALTALAVALLAAGCTLAPRYERPAAPVSGAFPADGVYAAQPGAAPGARSANGQAA
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
VDIGWREFFVDPRLQRLIEIALKNNRDLRVSVLNVEASRAQYQITRAGLFPTLSGTGTGT
EECCHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHEEEEHHCCCCCCCCCCCCE
IQRTPAGVSITGQPLISRTYNVGVSASWELDLFGRVQSLKDQALAQYFATAQARKAAEIS
EEECCCCCEEECCHHHHHHEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVASVADQYLTLLSTDDLLQVTQNTLKSARASYDLTKLQFDNGTGSELDLRQAQTVVETA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LASQQAQARARAQALNALVLLIGEPLPDDLPAGLPLNAQNLLTDIPAGLPSDLLTRRPDI
HHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
MQAEETLRAANANIGAARAAFFPKISLTSAFGTASPTLGGLFKAGTAAWSFAPNIALPIF
HHHHHHHHHHCCCCCHHHHHHCCCEEHHHHCCCCCCCHHHHHHHCCHHHCCCCCCEEEEE
EGGQNIANLDLAHVQKRIEIANYEKAIQSAFREVSDGLAARGTYDQQIAALERNEHAQQR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHH
RYDLSDLRYRNGVDSYLSVLTAQTDLYSAQQQLISARLARWTNLVDLYRALGGGWLERAG
HCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
ETPRPADAPVDYGKAAPAPASAVPAASASAPAAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]