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The map label for this gene is pncA [H]

Identifier: 126454510

GI number: 126454510

Start: 2306653

End: 2307291

Strand: Reverse

Name: pncA [H]

Synonym: BURPS1106A_2332

Alternate gene names: 126454510

Gene position: 2307291-2306653 (Counterclockwise)

Preceding gene: 126453618

Following gene: 126451726

Centisome position: 57.85

GC content: 72.3

Gene sequence:

>639_bases
ATGCAGCGCACCGACGATGTCCTTCTCGTGATCGACGTGCAGAACGATTTCATGCCGGGCGGCGCGCTCGCGGTCGCGCA
CGGCGACGAAGTCGTGCCGGCGATCAACCGGCTCGCCGCGCGCTTCGATCACGTCGTGCTGACGCAGGATTGGCATCCGC
GCGATCACGTGTCGTTCGCGGCGAACCATCCGGGCCGCGCGCCGTTCTCGACGCTCGCGCTGCCGTACGGCGAGCAGGTG
CTGTGGCCCGTCCACTGCGTGCAGGGCACGCACGGCGCGGCGCTGCACGGCGATCTCGACATCCCGCACGCGCGGCTCGT
GGTCCGCAAGGGGCATCGCGCGTCGGTCGACAGCTACTCGGCGTTCGTCGAGGCCGACCGCCGCACGCCGACCGGGCTTG
CCGGCTATCTGCGCGAAATCGGCGCCAAGCGCGTGTGGTGCTGCGGGCTCGCGACCGACTATTGCGTCGCGTGGTCCGCG
CTCGATGCGCGCGCCGCGGGCTTCGACGCGGCCGTGATCGAGGACGCGTGCCGCGCGATCGATCTCGACGGCTCGCTCGC
GCGCGCATGGGCGTCGCTCGCCGCGGCGGGCGTCGCGCGCGTGCAGTCGGCCGAGGTTTGCCCGGGCGTCGATCCGTAG

Upstream 100 bases:

>100_bases
GGCAGATCGCGCCGTCGATCGTCGAGCTCGCGAAGAGCGGGCGCGGTTTCAACGCGTGACGCGCGGCGGCGCGGCCCAGG
CCCATTGACGGAGCACCACG

Downstream 100 bases:

>100_bases
CCGCCGTCGTTCATTTCATCGCATATCGATCCACAGAATTCAGAATCAGGAGACTCGGATGACCGAAGCCGTAATCGTAT
CGACCGCCCGCACCGGGCTC

Product: pyrazinamidase/nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEVVPAINRLAARFDHVVLTQDWHPRDHVSFAANHPGRAPFSTLALPYGEQV
LWPVHCVQGTHGAALHGDLDIPHARLVVRKGHRASVDSYSAFVEADRRTPTGLAGYLREIGAKRVWCCGLATDYCVAWSA
LDARAAGFDAAVIEDACRAIDLDGSLARAWASLAAAGVARVQSAEVCPGVDP

Sequences:

>Translated_212_residues
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEVVPAINRLAARFDHVVLTQDWHPRDHVSFAANHPGRAPFSTLALPYGEQV
LWPVHCVQGTHGAALHGDLDIPHARLVVRKGHRASVDSYSAFVEADRRTPTGLAGYLREIGAKRVWCCGLATDYCVAWSA
LDARAAGFDAAVIEDACRAIDLDGSLARAWASLAAAGVARVQSAEVCPGVDP
>Mature_212_residues
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEVVPAINRLAARFDHVVLTQDWHPRDHVSFAANHPGRAPFSTLALPYGEQV
LWPVHCVQGTHGAALHGDLDIPHARLVVRKGHRASVDSYSAFVEADRRTPTGLAGYLREIGAKRVWCCGLATDYCVAWSA
LDARAAGFDAAVIEDACRAIDLDGSLARAWASLAAAGVARVQSAEVCPGVDP

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=195, Percent_Identity=43.0769230769231, Blast_Score=144, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI193206735, Length=222, Percent_Identity=32.4324324324324, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17543130, Length=222, Percent_Identity=32.4324324324324, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71999339, Length=197, Percent_Identity=34.010152284264, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321401, Length=193, Percent_Identity=34.7150259067358, Blast_Score=111, Evalue=8e-26,
Organism=Drosophila melanogaster, GI24648247, Length=200, Percent_Identity=38, Blast_Score=128, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22650; Mature: 22650

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEVVPAINRLAARFDHVVLTQDWHPRDHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCCCCCCCEEE
ANHPGRAPFSTLALPYGEQVLWPVHCVQGTHGAALHGDLDIPHARLVVRKGHRASVDSYS
CCCCCCCCHHHEECCCCCCEEEEEEEECCCCCCEEECCCCCCCCEEEEECCCCCCCHHHH
AFVEADRRTPTGLAGYLREIGAKRVWCCGLATDYCVAWSALDARAAGFDAAVIEDACRAI
HHHHCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
DLDGSLARAWASLAAAGVARVQSAEVCPGVDP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQRTDDVLLVIDVQNDFMPGGALAVAHGDEVVPAINRLAARFDHVVLTQDWHPRDHVSFA
CCCCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHHCEEEEECCCCCCCCCEEE
ANHPGRAPFSTLALPYGEQVLWPVHCVQGTHGAALHGDLDIPHARLVVRKGHRASVDSYS
CCCCCCCCHHHEECCCCCCEEEEEEEECCCCCCEEECCCCCCCCEEEEECCCCCCCHHHH
AFVEADRRTPTGLAGYLREIGAKRVWCCGLATDYCVAWSALDARAAGFDAAVIEDACRAI
HHHHCCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHEE
DLDGSLARAWASLAAAGVARVQSAEVCPGVDP
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]