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The map label for this gene is alkJ [H]

Identifier: 126454463

GI number: 126454463

Start: 3973857

End: 3975542

Strand: Reverse

Name: alkJ [H]

Synonym: BURPS1106A_4069

Alternate gene names: 126454463

Gene position: 3975542-3973857 (Counterclockwise)

Preceding gene: 126452315

Following gene: 126455450

Centisome position: 99.68

GC content: 69.28

Gene sequence:

>1686_bases
GTGACTACCGAACGTACGCTCGAAGGCGAATTCGATTATGTGATCGTCGGCGCCGGCACGGCGGGCTGCGTGCTCGCGAA
CCGGCTCACCGAGGATCCGGACGTGACCGTGCTGCTGCTCGAAGCCGGCGGCAGGGACGACTATCACTGGATCCACATCC
CGGTCGGCTATCTGTATTGCATCGGCAATCCGCGCACCGACTGGCTCTACAAGACCGAGCCCGAAGCGGGCCTGAACGGC
CGCGCGCTGTCGTATCCGCGCGGGCGCGTGCTGGGCGGCTCGTCGTCGATCAACGGAATGATCTACATGCGCGGCCAGCG
CGGCGATTACGACGACTGGGCGCGCGCCACGGGCGACGCGGGCTGGTCGTGGGACAGTGTGCTGCCCGTCTTCAGGCGCA
GCGAGGATCATCATGCGGGCGCGACCGACATGCACGGCGCGGGCGGCATGTGGCGCGTCGAGAAGCAGCGGCTGCGCTGG
GAGATTCTCGAGGCATTCTCGCAGGCCGCGCAGCAGACGGGCATTCCGGCCACCGACGATTTCAACCGCGGCGACAACAC
GGGCGTCGGCTATTTCGAAGTCAATCAGAAGCGCGGCATTCGCTGGAACGCGTCGAAGGCGTTCTTGCGCCCTGCGCTCG
CGCGGCCGAACCTCACGGTGATCACCGGCGCGCAGGCCGAGCGGCTCGTGTTCGACGGCAAGCGCTGCGCGGGGGTCGAA
TATCGCGGCGGCGGCGCGCCGTTCGTCGCGTGCGCGCGCGTCGAGGTGCTCGTTGCGTCGGGCGCGGTGAATTCGCCGCA
GTTGCTCGAATTGTCGGGCATCGGCGACGGCAGCCGGCTGCAGGCGCTCGGCATCGGCGTCATCGCGGATCTGCGCGGCG
TCGGCGAAAATCTTCAGGATCACTTGCAGTTGCGCATGGCGTTTCGCGTGCGCGGCGTGCGCACGCTGAACACGCTGTCC
GCGCACTGGTGGGGCAAGCTGTGGATCGGCGCGCAATACGCGCTGATGCAGCGCGGGCCGATGTCGATGGCGCCGTCGCA
ATTGGGCGCGTTCGCGAAATCGGACCCGAACGATCCGGCACTCGCGCGGCCCGATCTCGAATATCACGTGCAGCCGCTGT
CGCTCGAGCGCTTCGGCGAGCCGCTGCATCGCTTCAACGCGTTCACCGCGTCGGTCTGCCATCTGCGGCCGACGTCGCGC
GGCAGCGTCCATGCGGCGTCGCCGGATCCGGCGCGCGCGCCGTCGATTGCGCCGAACTATCTGTCGACCGATTACGATCG
CCATGTCGCGGCGAACGCGCTGCGCCTGACGCGCCGGATCGCCTCGGCGCCCGCGCTCGCGCGCTATGCACCCGAAGAAA
TCCTGCCGGGCGCCCGGTATGTGAGCGAAGCGGAGCTGATCGCCGCGGCGGGCGCCGTGGGCACGACGATTTTCCATCCG
GTCGGCACGTGCCGGATGGGGCGCGCCGACGATCCGGACGCCGTCGTCGATAGCCGCCTGCGCGTGCGCGGCGTGACGGG
GCTGCGGGTCGTCGACGCGTCGGTGATGCCGACGATCACGTCGGGCAACACGAACTCCCCGACGCTGATGATCGCCGAGC
GCGCGAGCGACATGATTCGCGCGGATCGCCGCGGCGCATCGGAGCGCGGCGCGAGCGCGCGCGCCGAAGCCGTGCTGCCG
ACGTAA

Upstream 100 bases:

>100_bases
GCGCGCCGGCGATCTGCGATCGGCGCGCGGCTCGGCGCGAAGGCGCGCGACGGCGGTCCGCCAAGAAACCGCCGCATCGG
AATCGAGGAGACGGCAGATC

Downstream 100 bases:

>100_bases
AAGTGACACGATCCATTGAAAGAATCGGCCACGAATCGAAATAAATAACCGGCTGAGGGTTAACCCAAAAGCAAAGCGGC
ACTCTCGCGCGCACTCGATC

Product: GMC family oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MTTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGRDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNG
RALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDAGWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRW
EILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
YRGGGAPFVACARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQDHLQLRMAFRVRGVRTLNTLS
AHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALARPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSR
GSVHAASPDPARAPSIAPNYLSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
VGTCRMGRADDPDAVVDSRLRVRGVTGLRVVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGASERGASARAEAVLP
T

Sequences:

>Translated_561_residues
MTTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGRDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNG
RALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDAGWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRW
EILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
YRGGGAPFVACARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQDHLQLRMAFRVRGVRTLNTLS
AHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALARPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSR
GSVHAASPDPARAPSIAPNYLSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
VGTCRMGRADDPDAVVDSRLRVRGVTGLRVVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGASERGASARAEAVLP
T
>Mature_560_residues
TTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGRDDYHWIHIPVGYLYCIGNPRTDWLYKTEPEAGLNGR
ALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDAGWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRWE
ILEAFSQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVEY
RGGGAPFVACARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQDHLQLRMAFRVRGVRTLNTLSA
HWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPALARPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRG
SVHAASPDPARAPSIAPNYLSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHPV
GTCRMGRADDPDAVVDSRLRVRGVTGLRVVDASVMPTITSGNTNSPTLMIAERASDMIRADRRGASERGASARAEAVLPT

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=42.0074349442379, Blast_Score=397, Evalue=1e-110,
Organism=Escherichia coli, GI1786503, Length=538, Percent_Identity=39.5910780669145, Blast_Score=366, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17532301, Length=559, Percent_Identity=34.1681574239714, Blast_Score=321, Evalue=8e-88,
Organism=Drosophila melanogaster, GI45549471, Length=571, Percent_Identity=37.4781085814361, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI45551458, Length=571, Percent_Identity=37.4781085814361, Blast_Score=335, Evalue=3e-92,
Organism=Drosophila melanogaster, GI24642055, Length=582, Percent_Identity=36.9415807560137, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI24642042, Length=575, Percent_Identity=35.8260869565217, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=36.0281195079086, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI24642048, Length=577, Percent_Identity=34.4887348353553, Blast_Score=301, Evalue=9e-82,
Organism=Drosophila melanogaster, GI18859995, Length=572, Percent_Identity=35.4895104895105, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24650267, Length=568, Percent_Identity=34.1549295774648, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI17137792, Length=556, Percent_Identity=33.9928057553957, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24642039, Length=571, Percent_Identity=34.6760070052539, Blast_Score=276, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24642037, Length=573, Percent_Identity=32.8097731239092, Blast_Score=265, Evalue=9e-71,
Organism=Drosophila melanogaster, GI24642051, Length=573, Percent_Identity=32.4607329842932, Blast_Score=258, Evalue=1e-68,
Organism=Drosophila melanogaster, GI18859993, Length=574, Percent_Identity=30.6620209059233, Blast_Score=253, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24642035, Length=303, Percent_Identity=40.2640264026403, Blast_Score=211, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24642057, Length=602, Percent_Identity=29.0697674418605, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=29.0378006872852, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 60766; Mature: 60635

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGRDDYHWIHIPVGYLYC
CCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEEEEE
IGNPRTDWLYKTEPEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDA
CCCCCCCCEEECCCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHCCCC
GWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRWEILEAFSQAAQQTGIPATDD
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
CCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEE
YRGGGAPFVACARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQD
ECCCCCCEEEHEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHH
HLQLRMAFRVRGVRTLNTLSAHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPA
HHHHHHHHHHCCCHHHHHHHHCCCEEEEECCEEHHHCCCCCCCCHHHHHHHHCCCCCCCC
LARPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRGSVHAASPDPARAPSIAPNY
CCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEEECCCCCCCCCCCCCCC
LSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
CCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHCC
VGTCRMGRADDPDAVVDSRLRVRGVTGLRVVDASVMPTITSGNTNSPTLMIAERASDMIR
CCCCCCCCCCCCHHHHCCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHH
ADRRGASERGASARAEAVLPT
HHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVTVLLLEAGGRDDYHWIHIPVGYLYC
CCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEEEEEEE
IGNPRTDWLYKTEPEAGLNGRALSYPRGRVLGGSSSINGMIYMRGQRGDYDDWARATGDA
CCCCCCCCEEECCCCCCCCCCEECCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHCCCC
GWSWDSVLPVFRRSEDHHAGATDMHGAGGMWRVEKQRLRWEILEAFSQAAQQTGIPATDD
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FNRGDNTGVGYFEVNQKRGIRWNASKAFLRPALARPNLTVITGAQAERLVFDGKRCAGVE
CCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCCEE
YRGGGAPFVACARVEVLVASGAVNSPQLLELSGIGDGSRLQALGIGVIADLRGVGENLQD
ECCCCCCEEEHEEEEEEEECCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHHHCCCHHH
HLQLRMAFRVRGVRTLNTLSAHWWGKLWIGAQYALMQRGPMSMAPSQLGAFAKSDPNDPA
HHHHHHHHHHCCCHHHHHHHHCCCEEEEECCEEHHHCCCCCCCCHHHHHHHHCCCCCCCC
LARPDLEYHVQPLSLERFGEPLHRFNAFTASVCHLRPTSRGSVHAASPDPARAPSIAPNY
CCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEEECCCCCCCCCCCCCCC
LSTDYDRHVAANALRLTRRIASAPALARYAPEEILPGARYVSEAELIAAAGAVGTTIFHP
CCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCHHHHHHHHHHHCCHHHHHHHCC
VGTCRMGRADDPDAVVDSRLRVRGVTGLRVVDASVMPTITSGNTNSPTLMIAERASDMIR
CCCCCCCCCCCCHHHHCCCEEEECCCCEEEECCCCCCEEECCCCCCCEEEEEHHHHHHHH
ADRRGASERGASARAEAVLPT
HHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]