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The map label for this gene is glgX [H]

Identifier: 126454362

GI number: 126454362

Start: 1516151

End: 1518259

Strand: Reverse

Name: glgX [H]

Synonym: BURPS1106A_1565

Alternate gene names: 126454362

Gene position: 1518259-1516151 (Counterclockwise)

Preceding gene: 126454792

Following gene: 126452473

Centisome position: 38.07

GC content: 71.17

Gene sequence:

>2109_bases
ATGCCGACACCCACGCCCGATCGCCTCGAACCGGGGCGCAGCTATCCGCTCGGCGCGCACTGGGACGGGCTCGGCGTGAA
CTTCGCGGTATTTTCCGCGCACGCCCGCCGGGTTCAGCTCTGCCTGTTCGATCCGACCGGACGCCGCGAGATCGCGCGCC
TCGATCTGCCCGAATGCACGGACGAAATCTGGCACGGCTACCTGCCCGACGCGCATCCGGGCACGGTCTACGCGTTCCGC
GCGGACGGCGCGTACGAGCCGCAGCACGGGCACCGCTTCAATCCGCACAAGCTGCTGCTCGATCCGTACGCGCGTAAGCT
CGTCGGGCAGTTTCGCTGGTCCGACGCGCTGTTCGGCTACCGGCTGCATTCGAGTCGCGCGGACTTGTCGATCGACCGGC
GCGATTCGGCGCCCGCGATGCCGAAATGCGTGGTCGTCGACGAGGCCGTCGACTGGAGCACCGACCGCCGCCCGTGCGTG
CCGTGGCGCGATACGGTGATCTACGAAGCCCACGTGCGCGGCGTGTCGATGCGGCGCGCCGAGCTGCGCGCGCCGGAACG
GGGCACGTTCGCCGCGCTCGCCTGCCCCGCGTTCATCGATCACCTGCTCGCACTCGGCGTGACGAGCGTCGAGCTGCTGC
CGGTGCACGCGTTCATGCACGCGCGCGCGCTCGTCAATCTCGGGCTGTGCAACTACTGGGGCTACGACACCGCCGCGTTC
TTCGCGCCCGAGCCCTCGTATCTCGCGACGCGCCGGCTCGACGAGATGCGCATCGCGATCCGCCAGTTGCACGCGGCGGG
CATCGAAGTGCTGCTCGACGTCGTCTACAACCATACGTGCGAGGGCAATCAGCTCGGCCCGACGATCTGCTGGCGCGGCC
TCGACAACGCGAGCTACTACCGGCTCGCGCCGGACGACCCGCGCCGCCACATCGACGAGACGGGCTGCGGCAACACGCTC
GACCTGTCGCACCCGCGCGTGCTGCAGATGGTGATGGATTCGCTGCGCTACTGGTCGAACGCGTTCAACGTCGACGGCTT
TCGCTTCGATCTCTGCGCGACGCTCGGCCGCGAGGCGGCCGGCTTCGATCCGGGCGCGGGCTTTTTCGACGCGCTGCGTC
AGGACCCCGTGCTCGCCACGCGCAAGCTGATCGCCGAGCCGTGGGACGTCGGCCCGGGCGGCTATCAGCTCGGCCGCCAT
CCGGCCGGCTTCGCCGAGTGGAACGACCGCTTTCGCGACACCGTGCGGCGCTTCTGGCGCGGCGACGCCGGCATGCGGCC
GGAGCTCGCCGCGCGGCTCGCGGGCTCGGCCGACCTGTTCAACCACCACGGGCGGCGGACCTGGTCGACCGTGAACTTCG
TCGCCGCGCACGACGGCTTCACGCTTGCCGATCTCGTCTCATACGCGGCCAAGCACAACGACGCGAACGGCGAAGACAAC
CGCGACGGGCGCGACGACAACTGCAGCGCGAACTGGGGCGCCGAGGGGCCCACCGAAGACAGACAGGTGCGGGCCGTGCG
CGCGCGCGTCGCGCGCTCGCTGCTCACGACGCTGTTCGCCGCGCTCGGCACGCCGATGCTGCTCGGCGGCGACGAGTTCG
GGCGCACGCAGCAGGGCAACAACAACGCGTATTGCCAGGATAACGAACTGTCGTGGTTCGACTGGAGCGCGGCCGAATCG
GCGGACGGCCGCACGATGATTCACTTCGTCGGCCGGCTCGCGGCGCTGCGGCGCGCGTATCCGGTGCTGTCCGAGCCGCG
CTATCCGTCGGGCGAGCGCGACGGCGCGCCCGGCTTGCACGAGATCGACTGGTTCGACGAGCGCGGAGACGCGCTGACGG
TGCCCGCCTGGCACGACCGCGAGCGGCGCGCGCTGACGATGCGGCGGGTCGGCAACGGACGCGCGGGGCGGCCGGAGGCG
TTGCTGCTGATGCTGAACGCGTCGTCCGACGCGATCGTGTTCCATGCGCCGCCGCCCTCGCTCGTCTACCGCATTCTGCT
CGACAGCGCGGCGCCCGACGCGGCGCCGCGGCTGATGCCCGCCGACGGCGTCGAGATCGCCGCGCACGCCGCGGTGATCG
CGGCGGCGTCGGTGCCCGAGGCCGGCTGA

Upstream 100 bases:

>100_bases
GCGCTGCGCCTGCCGCCGCTCGCGACGCTATGGCTGCGCCCCGCCTGACGCCGCGCGGCGCCCGCCGCACGCACGAACCG
CCACGAGGAGGAGCACGACG

Downstream 100 bases:

>100_bases
GCCGTCCGCTTCGCGAGGAACCGCCGATGAACGATTCGACCGCCACGCATGCGTATGCGCTGCCCTTCGGCGCGACGTGC
GTCGACGCGCGGCACACGCG

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MPTPTPDRLEPGRSYPLGAHWDGLGVNFAVFSAHARRVQLCLFDPTGRREIARLDLPECTDEIWHGYLPDAHPGTVYAFR
ADGAYEPQHGHRFNPHKLLLDPYARKLVGQFRWSDALFGYRLHSSRADLSIDRRDSAPAMPKCVVVDEAVDWSTDRRPCV
PWRDTVIYEAHVRGVSMRRAELRAPERGTFAALACPAFIDHLLALGVTSVELLPVHAFMHARALVNLGLCNYWGYDTAAF
FAPEPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTICWRGLDNASYYRLAPDDPRRHIDETGCGNTL
DLSHPRVLQMVMDSLRYWSNAFNVDGFRFDLCATLGREAAGFDPGAGFFDALRQDPVLATRKLIAEPWDVGPGGYQLGRH
PAGFAEWNDRFRDTVRRFWRGDAGMRPELAARLAGSADLFNHHGRRTWSTVNFVAAHDGFTLADLVSYAAKHNDANGEDN
RDGRDDNCSANWGAEGPTEDRQVRAVRARVARSLLTTLFAALGTPMLLGGDEFGRTQQGNNNAYCQDNELSWFDWSAAES
ADGRTMIHFVGRLAALRRAYPVLSEPRYPSGERDGAPGLHEIDWFDERGDALTVPAWHDRERRALTMRRVGNGRAGRPEA
LLLMLNASSDAIVFHAPPPSLVYRILLDSAAPDAAPRLMPADGVEIAAHAAVIAAASVPEAG

Sequences:

>Translated_702_residues
MPTPTPDRLEPGRSYPLGAHWDGLGVNFAVFSAHARRVQLCLFDPTGRREIARLDLPECTDEIWHGYLPDAHPGTVYAFR
ADGAYEPQHGHRFNPHKLLLDPYARKLVGQFRWSDALFGYRLHSSRADLSIDRRDSAPAMPKCVVVDEAVDWSTDRRPCV
PWRDTVIYEAHVRGVSMRRAELRAPERGTFAALACPAFIDHLLALGVTSVELLPVHAFMHARALVNLGLCNYWGYDTAAF
FAPEPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTICWRGLDNASYYRLAPDDPRRHIDETGCGNTL
DLSHPRVLQMVMDSLRYWSNAFNVDGFRFDLCATLGREAAGFDPGAGFFDALRQDPVLATRKLIAEPWDVGPGGYQLGRH
PAGFAEWNDRFRDTVRRFWRGDAGMRPELAARLAGSADLFNHHGRRTWSTVNFVAAHDGFTLADLVSYAAKHNDANGEDN
RDGRDDNCSANWGAEGPTEDRQVRAVRARVARSLLTTLFAALGTPMLLGGDEFGRTQQGNNNAYCQDNELSWFDWSAAES
ADGRTMIHFVGRLAALRRAYPVLSEPRYPSGERDGAPGLHEIDWFDERGDALTVPAWHDRERRALTMRRVGNGRAGRPEA
LLLMLNASSDAIVFHAPPPSLVYRILLDSAAPDAAPRLMPADGVEIAAHAAVIAAASVPEAG
>Mature_701_residues
PTPTPDRLEPGRSYPLGAHWDGLGVNFAVFSAHARRVQLCLFDPTGRREIARLDLPECTDEIWHGYLPDAHPGTVYAFRA
DGAYEPQHGHRFNPHKLLLDPYARKLVGQFRWSDALFGYRLHSSRADLSIDRRDSAPAMPKCVVVDEAVDWSTDRRPCVP
WRDTVIYEAHVRGVSMRRAELRAPERGTFAALACPAFIDHLLALGVTSVELLPVHAFMHARALVNLGLCNYWGYDTAAFF
APEPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTICWRGLDNASYYRLAPDDPRRHIDETGCGNTLD
LSHPRVLQMVMDSLRYWSNAFNVDGFRFDLCATLGREAAGFDPGAGFFDALRQDPVLATRKLIAEPWDVGPGGYQLGRHP
AGFAEWNDRFRDTVRRFWRGDAGMRPELAARLAGSADLFNHHGRRTWSTVNFVAAHDGFTLADLVSYAAKHNDANGEDNR
DGRDDNCSANWGAEGPTEDRQVRAVRARVARSLLTTLFAALGTPMLLGGDEFGRTQQGNNNAYCQDNELSWFDWSAAESA
DGRTMIHFVGRLAALRRAYPVLSEPRYPSGERDGAPGLHEIDWFDERGDALTVPAWHDRERRALTMRRVGNGRAGRPEAL
LLMLNASSDAIVFHAPPPSLVYRILLDSAAPDAAPRLMPADGVEIAAHAAVIAAASVPEAG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=618, Percent_Identity=47.2491909385113, Blast_Score=531, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 77954; Mature: 77823

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPTPDRLEPGRSYPLGAHWDGLGVNFAVFSAHARRVQLCLFDPTGRREIARLDLPECT
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCHHHCCCCHHHH
DEIWHGYLPDAHPGTVYAFRADGAYEPQHGHRFNPHKLLLDPYARKLVGQFRWSDALFGY
HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCEEE
RLHSSRADLSIDRRDSAPAMPKCVVVDEAVDWSTDRRPCVPWRDTVIYEAHVRGVSMRRA
EEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHCCCHHHHH
ELRAPERGTFAALACPAFIDHLLALGVTSVELLPVHAFMHARALVNLGLCNYWGYDTAAF
HHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEE
FAPEPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTICWRGLDNASYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCEE
RLAPDDPRRHIDETGCGNTLDLSHPRVLQMVMDSLRYWSNAFNVDGFRFDLCATLGREAA
EECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCCCC
GFDPGAGFFDALRQDPVLATRKLIAEPWDVGPGGYQLGRHPAGFAEWNDRFRDTVRRFWR
CCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDAGMRPELAARLAGSADLFNHHGRRTWSTVNFVAAHDGFTLADLVSYAAKHNDANGEDN
CCCCCCHHHHHHHCCCHHHHHCCCCCEECEEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
RDGRDDNCSANWGAEGPTEDRQVRAVRARVARSLLTTLFAALGTPMLLGGDEFGRTQQGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCC
NNAYCQDNELSWFDWSAAESADGRTMIHFVGRLAALRRAYPVLSEPRYPSGERDGAPGLH
CCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EIDWFDERGDALTVPAWHDRERRALTMRRVGNGRAGRPEALLLMLNASSDAIVFHAPPPS
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCHH
LVYRILLDSAAPDAAPRLMPADGVEIAAHAAVIAAASVPEAG
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTPTPDRLEPGRSYPLGAHWDGLGVNFAVFSAHARRVQLCLFDPTGRREIARLDLPECT
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCHHHCCCCHHHH
DEIWHGYLPDAHPGTVYAFRADGAYEPQHGHRFNPHKLLLDPYARKLVGQFRWSDALFGY
HHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCEEE
RLHSSRADLSIDRRDSAPAMPKCVVVDEAVDWSTDRRPCVPWRDTVIYEAHVRGVSMRRA
EEECCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEHHCCCHHHHH
ELRAPERGTFAALACPAFIDHLLALGVTSVELLPVHAFMHARALVNLGLCNYWGYDTAAF
HHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEE
FAPEPSYLATRRLDEMRIAIRQLHAAGIEVLLDVVYNHTCEGNQLGPTICWRGLDNASYY
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCEE
RLAPDDPRRHIDETGCGNTLDLSHPRVLQMVMDSLRYWSNAFNVDGFRFDLCATLGREAA
EECCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCCCC
GFDPGAGFFDALRQDPVLATRKLIAEPWDVGPGGYQLGRHPAGFAEWNDRFRDTVRRFWR
CCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDAGMRPELAARLAGSADLFNHHGRRTWSTVNFVAAHDGFTLADLVSYAAKHNDANGEDN
CCCCCCHHHHHHHCCCHHHHHCCCCCEECEEEEEEECCCCCHHHHHHHHHHCCCCCCCCC
RDGRDDNCSANWGAEGPTEDRQVRAVRARVARSLLTTLFAALGTPMLLGGDEFGRTQQGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCC
NNAYCQDNELSWFDWSAAESADGRTMIHFVGRLAALRRAYPVLSEPRYPSGERDGAPGLH
CCCCEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
EIDWFDERGDALTVPAWHDRERRALTMRRVGNGRAGRPEALLLMLNASSDAIVFHAPPPS
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEECCCHH
LVYRILLDSAAPDAAPRLMPADGVEIAAHAAVIAAASVPEAG
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]