Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 126454342

GI number: 126454342

Start: 106807

End: 108210

Strand: Direct

Name: trmE

Synonym: BURPS1106A_0111

Alternate gene names: 126454342

Gene position: 106807-108210 (Clockwise)

Preceding gene: 126452170

Following gene: 126455326

Centisome position: 2.68

GC content: 69.02

Gene sequence:

>1404_bases
ATGCTCGCCACTGATTCCGATCCGATCGTCGCCATTGCCACCGCGTCAGGCCGAGGCGGGATCGGCGTCGTGCGCCTTTC
GCTTGGGCGAGCGGGGGAAGCGGCGGCGCGCGCGCTGAGCGATGCGCTGTGCGGTGCGCGACTCATGCCGAGGCATGCGA
GCTACGTGCCGTTTCTCGACGGCGCGGGCGAGCCGCTCGATCGCGGCATCGCGCTCTATTTCCCCGCGCCGCATTCGTAT
ACGGGCGAGCACGTGATCGAGCTGCAAGGCCACGGCGGGCCGATCGTGCTGCAGCTCTTGCTGCAGCGCTGCCTCGACGC
CGGGCGTGCGCATGGGCTGCGGCTCGCTGAGCCGGGCGAATTCACGCGCCGGGCGTTCCTGAACGACAAGCTCGACCTCG
CGCAGGCGGAGGCCGTCGCCGATCTGATCGAGGCGAGCACCGAGGCGGCTGCGCGTTCGGCGGGCCGCTCGCTCGACGGG
GCGTTCTCGCGCGACATCCATGCGCTCGTCGACGACGTGATCGCGCTGCGGATGCTCGTCGAGGCGACGCTCGATTTTCC
GGAGGAGGAGATCGACTTCCTGGAGGCGGCCGACGCGCGCGGCAAGCTCGCGCACATCCGCGAGCGGCTCGCGCACGTGC
TCGGCGACGCGCGGCAGGGGGCGTTGTTGCGCGAGGGGCTATCGGTCGTGCTTGCCGGGCAGCCGAACGTCGGCAAGTCG
TCGCTGTTGAATGCGCTTGCGGGCGCGGAACTGGCGATCGTCACGCCGATCGCCGGCACGACGCGCGACAAGGTTGCGCA
GACGATTCAGATCGAGGGCATTCCGTTGCACATTATCGATACGGCGGGCTTGCGCGAAACGGAGGACGAGGTCGAGAAGA
TCGGCATCGCGCGCACGTGGGGCGAGATCGAGCGGGCGGACGTCGTGCTGCACCTGCTCGATGCGAGAAGCGGGCTTGGC
CCGGGCGACGAGGCGATCGCCGCGCGCTTCCCGGACGGCGTGCCGGTGGTGCGCGTGCTGAACAAGACGGATCTGACGGG
CGCGCCGGCATCGGTGACGCGGACAGGCGGCGGCGCGGCGCGCGCGGACGTATGTGAAGTGCGGCTGTCGGCGAAGCGCG
GCGACGGGATCGATTTGCTGCGCGGCGAGCTGCTGCGGATCGCCGGTTGGCAGGCGGGTGCGGAAAGCGTGTATCTCGCC
CGCGAGCGGCATCTGATCGCGCTGCGCGCGGCGCAGGCGCATCTCGCGCGGGCGGCGGAGCACGCGGAGCAGAATGCGCA
GGCGCTGGATCTCTTCGCCGAGGAACTGCGGCTCGCGCAGGAGCGGCTTAATTCGATCACCGGGGAGTTTACGTCCGATG
ATTTGTTGGGGGTGATTTTTAGTCGGTTTTGTATCGGAAAATAA

Upstream 100 bases:

>100_bases
TGGTCTGCGCAATACTATTGCGTGGCCGGGCTACAATTCGACATTCAAGGATATCCGCGGTTTCTTTGATTTCCATTTCA
TTTATTTCAGATTTTTTGCC

Downstream 100 bases:

>100_bases
GAAGTCGCCAAGCGACGGCAAAGGTTCACTGAAAAATCGCAATAAATCCTTATTGCGCAAGGCGTGTAGGCAAAAGGTTG
CCAACCTTCCATAATTGACA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MLATDSDPIVAIATASGRGGIGVVRLSLGRAGEAAARALSDALCGARLMPRHASYVPFLDGAGEPLDRGIALYFPAPHSY
TGEHVIELQGHGGPIVLQLLLQRCLDAGRAHGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVDDVIALRMLVEATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIIDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDARSGLG
PGDEAIAARFPDGVPVVRVLNKTDLTGAPASVTRTGGGAARADVCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLA
RERHLIALRAAQAHLARAAEHAEQNAQALDLFAEELRLAQERLNSITGEFTSDDLLGVIFSRFCIGK

Sequences:

>Translated_467_residues
MLATDSDPIVAIATASGRGGIGVVRLSLGRAGEAAARALSDALCGARLMPRHASYVPFLDGAGEPLDRGIALYFPAPHSY
TGEHVIELQGHGGPIVLQLLLQRCLDAGRAHGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVDDVIALRMLVEATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIIDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDARSGLG
PGDEAIAARFPDGVPVVRVLNKTDLTGAPASVTRTGGGAARADVCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLA
RERHLIALRAAQAHLARAAEHAEQNAQALDLFAEELRLAQERLNSITGEFTSDDLLGVIFSRFCIGK
>Mature_467_residues
MLATDSDPIVAIATASGRGGIGVVRLSLGRAGEAAARALSDALCGARLMPRHASYVPFLDGAGEPLDRGIALYFPAPHSY
TGEHVIELQGHGGPIVLQLLLQRCLDAGRAHGLRLAEPGEFTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDG
AFSRDIHALVDDVIALRMLVEATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIIDTAGLRETEDEVEKIGIARTWGEIERADVVLHLLDARSGLG
PGDEAIAARFPDGVPVVRVLNKTDLTGAPASVTRTGGGAARADVCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLA
RERHLIALRAAQAHLARAAEHAEQNAQALDLFAEELRLAQERLNSITGEFTSDDLLGVIFSRFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=482, Percent_Identity=37.9668049792531, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI193082988, Length=512, Percent_Identity=35.9375, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI193082993, Length=472, Percent_Identity=37.7118644067797, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=53.6324786324786, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI87082120, Length=117, Percent_Identity=46.1538461538462, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17507259, Length=436, Percent_Identity=29.3577981651376, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6323665, Length=429, Percent_Identity=30.3030303030303, Blast_Score=179, Evalue=7e-46,
Organism=Drosophila melanogaster, GI45550813, Length=498, Percent_Identity=33.1325301204819, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BURM7 (A3MS17)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001083053.1
- ProteinModelPortal:   A3MS17
- SMR:   A3MS17
- GeneID:   4893474
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_3547
- TIGR:   BMA10247_3547
- HOGENOM:   HBG746795
- ProtClustDB:   PRK05291
- BioCyc:   BMAL320389:BMA10247_3547-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 49454; Mature: 49454

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: BINDING 25-25 BINDING 87-87 BINDING 130-130 BINDING 467-467

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATDSDPIVAIATASGRGGIGVVRLSLGRAGEAAARALSDALCGARLMPRHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCCC
GAGEPLDRGIALYFPAPHSYTGEHVIELQGHGGPIVLQLLLQRCLDAGRAHGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVDDVIALRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIIDTAGLRETEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHCHHHHH
GEIERADVVLHLLDARSGLGPGDEAIAARFPDGVPVVRVLNKTDLTGAPASVTRTGGGAA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHEEECCCCCCCCCCCEEECCCCCH
RADVCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLARERHLIALRAAQAHLARAAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHCCCEEEHHHHHHHHHHHHH
HAEQNAQALDLFAEELRLAQERLNSITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLATDSDPIVAIATASGRGGIGVVRLSLGRAGEAAARALSDALCGARLMPRHASYVPFLD
CCCCCCCCEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCCCCCC
GAGEPLDRGIALYFPAPHSYTGEHVIELQGHGGPIVLQLLLQRCLDAGRAHGLRLAEPGE
CCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCH
FTRRAFLNDKLDLAQAEAVADLIEASTEAAARSAGRSLDGAFSRDIHALVDDVIALRMLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
EATLDFPEEEIDFLEAADARGKLAHIRERLAHVLGDARQGALLREGLSVVLAGQPNVGKS
HHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEECCCCCCHH
SLLNALAGAELAIVTPIAGTTRDKVAQTIQIEGIPLHIIDTAGLRETEDEVEKIGIARTW
HHHHHHCCCCEEEEECCCCCCHHHHHHEEEECCCEEEEEECCCCCCCHHHHHHHCHHHHH
GEIERADVVLHLLDARSGLGPGDEAIAARFPDGVPVVRVLNKTDLTGAPASVTRTGGGAA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHEEECCCCCCCCCCCEEECCCCCH
RADVCEVRLSAKRGDGIDLLRGELLRIAGWQAGAESVYLARERHLIALRAAQAHLARAAE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEHHCCCEEEHHHHHHHHHHHHH
HAEQNAQALDLFAEELRLAQERLNSITGEFTSDDLLGVIFSRFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA