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The map label for this gene is argT [H]

Identifier: 126454248

GI number: 126454248

Start: 2738203

End: 2738997

Strand: Reverse

Name: argT [H]

Synonym: BURPS1106A_2777

Alternate gene names: 126454248

Gene position: 2738997-2738203 (Counterclockwise)

Preceding gene: 126453866

Following gene: 126455392

Centisome position: 68.67

GC content: 65.53

Gene sequence:

>795_bases
ATGAAGTTGAACTGGCGGAAGATGGCCGCGCACGCGGTGGTGACGGCCACGGCCCTGGCGGCGGGCAGCGCGTTCGCCGC
CGACCTGAAGGAAATCCGCTTCGGCGTCGAGGCGTCGTATGCGCCGTTCGAGTACAAGACGCCGGACGGCAAGCTGGCGG
GCTTCGACATCGACATCGGCAACGCGGTGTGCGCGAAGCTGAAGGTCAAGTGCGTGTGGGTCGAGAACGCGTTCGACGGC
CTGATCCCGGCGCTGCAGGCGCGCAAGTTCGACGCGATCAACTCCGACATGACGATCACCGAGCAACGTCGGAAGGCAAT
CGATTTCACCGACCCGATCTACACGATCCCGAACCAGCTGATCGCGAAGAAGGGCAGCGGCCTGCTGCCGACCACCGCGT
CGCTGAAGGGCAAGCGCATCGGCGTGCTGCAGGGCACGATCCAGGAAGCGTACGCGAAGAAGCGCTGGGCGCCGGCGGGC
GTCGAAGTCGTGCCGTACCAGACGCAGGATCTCGCCTACGAAGACCTGAAGTCGGGCCGCCTCGACGCGACCTTCCAGGA
TTCGGAGGCCGGCGCGAAGGGCTTCCTGTCGAAGCCGCAGGGCGCGGGTTTCGCGTTCGCGGGCGACCACGTGAGCGACG
CCGAGATCCTCGGCACGGGCGTGGGCTTCGGCATGCGCAAGAACGACGCGCAGCTGAAGAGCGCAGTCAATCAGGCGCTC
AAGGAACTGAAAGCCGACGGCACGATCGACGGCCTCGCGAAGAAGTATTTCAGCGTGCCCGTGACGCTGAAGTAA

Upstream 100 bases:

>100_bases
ACGGCCGCCCCGCGCGGTTGTCCGGAAATTTCGACGCTGCAATGATGCGGCGGTCCGGTCGTTGACTGACATTGTTCGTT
CAAAGCGAGGAGAACACCTG

Downstream 100 bases:

>100_bases
TGCGTGCGGCGGGGGCGGTTCGCCGCCGCGCCGCGATGGGTTCGAATCGGCCGGCCGCTTCATCGAGCGGCCGGTTTTGT
TTTATCGGGGCGCGGCCGCA

Product: lysine-arginine-ornithine-binding periplasmic protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKLNWRKMAAHAVVTATALAAGSAFAADLKEIRFGVEASYAPFEYKTPDGKLAGFDIDIGNAVCAKLKVKCVWVENAFDG
LIPALQARKFDAINSDMTITEQRRKAIDFTDPIYTIPNQLIAKKGSGLLPTTASLKGKRIGVLQGTIQEAYAKKRWAPAG
VEVVPYQTQDLAYEDLKSGRLDATFQDSEAGAKGFLSKPQGAGFAFAGDHVSDAEILGTGVGFGMRKNDAQLKSAVNQAL
KELKADGTIDGLAKKYFSVPVTLK

Sequences:

>Translated_264_residues
MKLNWRKMAAHAVVTATALAAGSAFAADLKEIRFGVEASYAPFEYKTPDGKLAGFDIDIGNAVCAKLKVKCVWVENAFDG
LIPALQARKFDAINSDMTITEQRRKAIDFTDPIYTIPNQLIAKKGSGLLPTTASLKGKRIGVLQGTIQEAYAKKRWAPAG
VEVVPYQTQDLAYEDLKSGRLDATFQDSEAGAKGFLSKPQGAGFAFAGDHVSDAEILGTGVGFGMRKNDAQLKSAVNQAL
KELKADGTIDGLAKKYFSVPVTLK
>Mature_264_residues
MKLNWRKMAAHAVVTATALAAGSAFAADLKEIRFGVEASYAPFEYKTPDGKLAGFDIDIGNAVCAKLKVKCVWVENAFDG
LIPALQARKFDAINSDMTITEQRRKAIDFTDPIYTIPNQLIAKKGSGLLPTTASLKGKRIGVLQGTIQEAYAKKRWAPAG
VEVVPYQTQDLAYEDLKSGRLDATFQDSEAGAKGFLSKPQGAGFAFAGDHVSDAEILGTGVGFGMRKNDAQLKSAVNQAL
KELKADGTIDGLAKKYFSVPVTLK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=241, Percent_Identity=51.4522821576763, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1788648, Length=232, Percent_Identity=48.7068965517241, Blast_Score=250, Evalue=6e-68,
Organism=Escherichia coli, GI1787085, Length=245, Percent_Identity=37.1428571428571, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=33.8775510204082, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788228, Length=275, Percent_Identity=33.8181818181818, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787031, Length=237, Percent_Identity=31.6455696202532, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI1786876, Length=251, Percent_Identity=25.4980079681275, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28323; Mature: 28323

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNWRKMAAHAVVTATALAAGSAFAADLKEIRFGVEASYAPFEYKTPDGKLAGFDIDIG
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKVKCVWVENAFDGLIPALQARKFDAINSDMTITEQRRKAIDFTDPIYTIPNQL
CCEEEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCHHHCCHHH
IAKKGSGLLPTTASLKGKRIGVLQGTIQEAYAKKRWAPAGVEVVPYQTQDLAYEDLKSGR
HHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCC
LDATFQDSEAGAKGFLSKPQGAGFAFAGDHVSDAEILGTGVGFGMRKNDAQLKSAVNQAL
CCCEECCCCCCCCCCCCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHH
KELKADGTIDGLAKKYFSVPVTLK
HHHHCCCCHHHHHHHHHCCCCEEC
>Mature Secondary Structure
MKLNWRKMAAHAVVTATALAAGSAFAADLKEIRFGVEASYAPFEYKTPDGKLAGFDIDIG
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEECC
NAVCAKLKVKCVWVENAFDGLIPALQARKFDAINSDMTITEQRRKAIDFTDPIYTIPNQL
CCEEEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCHHHCCHHH
IAKKGSGLLPTTASLKGKRIGVLQGTIQEAYAKKRWAPAGVEVVPYQTQDLAYEDLKSGR
HHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCC
LDATFQDSEAGAKGFLSKPQGAGFAFAGDHVSDAEILGTGVGFGMRKNDAQLKSAVNQAL
CCCEECCCCCCCCCCCCCCCCCCEEEECCCCCCHHHEECCCCCCCCCCHHHHHHHHHHHH
KELKADGTIDGLAKKYFSVPVTLK
HHHHCCCCHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]