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The map label for this gene is ptsI [H]

Identifier: 126454191

GI number: 126454191

Start: 459239

End: 461029

Strand: Direct

Name: ptsI [H]

Synonym: BURPS1106A_0493

Alternate gene names: 126454191

Gene position: 459239-461029 (Clockwise)

Preceding gene: 126452660

Following gene: 126452970

Centisome position: 11.51

GC content: 67.62

Gene sequence:

>1791_bases
GTGATCAAGGAGGTGCGCGTGTCTTTCACGCTGCATGGCATTCCCGTTTCAAGAGGTATCGCGATCGGACGAGCGTATCT
GATCGCGCCGGCGGCGCTCGACGTCGCGCATTACCTGATCGAGGCCGAACGGATCGAGGCCGAGATCGAGCGCTTTCGCA
CGGCGCTCGGCGCCGTGCGGCGCGAACTCGACGTGCTGCGCGCGGATCTGACCGACGACACCCCGACCGAGGTCGCCGCG
TTCATCGACGTGCACGCGATGATCCTCGGCGACGCGATGCTCGTGCAGGAAACCATCGATCTCATCCGCACGCGCCGCTA
CAACGTCGAATGGGCGCTGACCGAGCAGCTCGACGTGCTCGCCGGCCACTTCGACGACATCGAGGACGAGTACCTGCGCG
AGCGCAAGGCCGATATCGAACAGGTGGTCGAGCGGGTGCTGAAGGCGCTCGCGGGCGCGCCGTCCGCCGCGCAGGCGCTC
GACCGCGCGGCGGGCAACGGTCGCGACGAGATGATCGTCGTCGCGCACGACATCGCGCCCGCCGACATGATGCAGTTCAA
GACGCAGTCGTTCCAGGCGTTCGTCACCGACTTGGGCGGGCGCACCTCGCACACGGCGATCGTCGCGCGCAGCCTCGGGA
TTCCGGCCGCGGTCGGCGTCCAGCACGCGAGCGCGCTGATTCGCCAGGACGATCTCATCATCGTCGACGGCGATCAGGGG
ATCGTGATCGTCGATCCCGCGCCGATCGTGCTCGAGGAATACTCGTACCGGCAATCGGAGAAGGCGCTCGAGCAGCGCAA
GCTGCAGCGCCTGAAGTTCTCGCCCGCGCAGACGCTGTGCGGCACGAAGATCGATCTGCTCGCGAACATCGAGCTGCCCG
ACGACGCGAAGGCGGCCGTCGACGCGGGCGCGGTCGGCGTCGGCCTGTTCCGCACCGAGTTCCTGTTCATGAGCAAGGTG
CGGATGCCGGAGGAAGAGGAGCAGTTCGCCGCGTACAAGCGCGCGGTCGAGCTGATGCACGGGATGCCCGTCACGATCCG
CACGATCGACGTCGGCGCGGACAAGCCGCTCGATGTGCACGACGAAGGCTACGAGACCGCGCCGAATCCCGCGCTCGGCC
TGCGCGCGATCCGCTGGAGCCTGTCGGAGCCGCAGATGTTCCTCACGCAGTTGCGCGCGATCCTGCGCGCGTCGGCGTTC
GGCCAGGTGAAGATCCTGGTGCCGATGCTCGCGCACGCGCAGGAGATCGACCAGACGCTCGACCTCATCAACGAGGCGAA
GCGGCAACTCGACGCGGCGGGGCTCGCATACGATCCGAACGTGCGGGTCGGCGCGATGATCGAGATTCCGGCCGCGGCGA
TCGCGCTGCCGCTGTTCCTCAAGCGCGTCGATTTCCTGTCGATCGGCACGAACGACCTGATCCAGTACACGCTCGCGATC
GACCGCGCGGACAACGCGGTCGCGCATCTGTACGATCCGCTGCATCCGGCTGTGCTGCATCTGATCGCGTTCACGCTGCG
CGAAGCGAAGCGCGCGGGCGTGCCGGTGTCGGTGTGCGGCGAGATGGCGGGCGATCCGGCGCTCACGCGTCTGTTGCTCG
GCATGGGGCTCACCGAGTTCTCGATGCATCCGAGCCAGTTGCTCGTCGTCAAGCAGGAAATCCTGCGCGCGCACCTGAAG
GCGCTCGAGAAGCCGACCGCGGACGTGCTCGCATCGTTCGAACCGGAAGAAGTGCAGGCCGCGCTCGCGCGGCTCGCGAG
CGCGGAGCCGAGGGCGGACGTCGCCGCGTGA

Upstream 100 bases:

>100_bases
CGTGCCGCGATGCTGCGCCGCACGCAGTCATGCGCGTATGCGGAATTTATAATCGTCGGTCGGGGTCATAAGCGCATCGC
AGCGGTGCGCCGCGACTCAC

Downstream 100 bases:

>100_bases
TCGCGGGGCGAGCCGAGCGGGCGCGCGTGGCGCCGGCGGGGGCTGAAGCGGGGAGGCGGGGCGCGGCCGCCCGCCCGTTT
GGGTTCGATTGCAAGCGCGG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MIKEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRTALGAVRRELDVLRADLTDDTPTEVAA
FIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDDIEDEYLRERKADIEQVVERVLKALAGAPSAAQAL
DRAAGNGRDEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQG
IVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVGVGLFRTEFLFMSKV
RMPEEEEQFAAYKRAVELMHGMPVTIRTIDVGADKPLDVHDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAF
GQVKILVPMLAHAQEIDQTLDLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAI
DRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLK
ALEKPTADVLASFEPEEVQAALARLASAEPRADVAA

Sequences:

>Translated_596_residues
MIKEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRTALGAVRRELDVLRADLTDDTPTEVAA
FIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDDIEDEYLRERKADIEQVVERVLKALAGAPSAAQAL
DRAAGNGRDEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQG
IVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVGVGLFRTEFLFMSKV
RMPEEEEQFAAYKRAVELMHGMPVTIRTIDVGADKPLDVHDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAF
GQVKILVPMLAHAQEIDQTLDLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAI
DRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLK
ALEKPTADVLASFEPEEVQAALARLASAEPRADVAA
>Mature_596_residues
MIKEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRTALGAVRRELDVLRADLTDDTPTEVAA
FIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDVLAGHFDDIEDEYLRERKADIEQVVERVLKALAGAPSAAQAL
DRAAGNGRDEMIVVAHDIAPADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQG
IVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAVDAGAVGVGLFRTEFLFMSKV
RMPEEEEQFAAYKRAVELMHGMPVTIRTIDVGADKPLDVHDEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAF
GQVKILVPMLAHAQEIDQTLDLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAI
DRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEFSMHPSQLLVVKQEILRAHLK
ALEKPTADVLASFEPEEVQAALARLASAEPRADVAA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=38.1786339754816, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789193, Length=537, Percent_Identity=34.4506517690875, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI48994992, Length=519, Percent_Identity=31.5992292870906, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI1788726, Length=585, Percent_Identity=27.6923076923077, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787994, Length=413, Percent_Identity=27.8450363196126, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65191; Mature: 65191

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRTALGAVR
CCCEEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELDVLRADLTDDTPTEVAAFIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDVL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
AGHFDDIEDEYLRERKADIEQVVERVLKALAGAPSAAQALDRAAGNGRDEMIVVAHDIAP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCC
ADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCC
IVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHH
DAGAVGVGLFRTEFLFMSKVRMPEEEEQFAAYKRAVELMHGMPVTIRTIDVGADKPLDVH
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
DEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILVPMLAHAQEIDQTL
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAI
HHHHHHHHHHHHCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEE
DRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEF
ECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCHHH
SMHPSQLLVVKQEILRAHLKALEKPTADVLASFEPEEVQAALARLASAEPRADVAA
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIKEVRVSFTLHGIPVSRGIAIGRAYLIAPAALDVAHYLIEAERIEAEIERFRTALGAVR
CCCEEEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RELDVLRADLTDDTPTEVAAFIDVHAMILGDAMLVQETIDLIRTRRYNVEWALTEQLDVL
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
AGHFDDIEDEYLRERKADIEQVVERVLKALAGAPSAAQALDRAAGNGRDEMIVVAHDIAP
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEECCCC
ADMMQFKTQSFQAFVTDLGGRTSHTAIVARSLGIPAAVGVQHASALIRQDDLIIVDGDQG
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCC
IVIVDPAPIVLEEYSYRQSEKALEQRKLQRLKFSPAQTLCGTKIDLLANIELPDDAKAAV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCCHHHHH
DAGAVGVGLFRTEFLFMSKVRMPEEEEQFAAYKRAVELMHGMPVTIRTIDVGADKPLDVH
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
DEGYETAPNPALGLRAIRWSLSEPQMFLTQLRAILRASAFGQVKILVPMLAHAQEIDQTL
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
DLINEAKRQLDAAGLAYDPNVRVGAMIEIPAAAIALPLFLKRVDFLSIGTNDLIQYTLAI
HHHHHHHHHHHHCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHEEEEEEE
DRADNAVAHLYDPLHPAVLHLIAFTLREAKRAGVPVSVCGEMAGDPALTRLLLGMGLTEF
ECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCHHH
SMHPSQLLVVKQEILRAHLKALEKPTADVLASFEPEEVQAALARLASAEPRADVAA
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]