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The map label for this gene is tilS [H]

Identifier: 126454111

GI number: 126454111

Start: 2558148

End: 2559626

Strand: Reverse

Name: tilS [H]

Synonym: BURPS1106A_2593

Alternate gene names: 126454111

Gene position: 2559626-2558148 (Counterclockwise)

Preceding gene: 126454581

Following gene: 126451837

Centisome position: 64.18

GC content: 73.09

Gene sequence:

>1479_bases
GTGATTCCCCCGCATGAATTTTCCGCCGAGCGCGTCGTGTTCGACGCGCTCGGCGTCGCGCTGTCCGCGTTGCCCGACGA
CACGCCGATCGCGATCGCGTACAGCGGCGGCCTCGATTCGACCGTGCTGTTGCACGCGGCCGCGCGCATCGCGGGCGCCG
GACGTTGCATCGCGTTGCATGTTCACCACGGCCTGAGCGCGAACGCCGACGCATGGCTCGCGCATTGCGCGGAAACGGCG
CAAGCGCTCGGCGCGCGCTTCGACGCGGCGCGCGTCGACGTGCCGCGCGCGAGCGGGCAGGGCATCGAGGCGAGCGCGCG
CGATGCGCGCTATCGCGCGCTCGAGACGATGTGCGCGCGATACGGCGCGCGCACGCTGTGGCTCGCGCAGCATGCGGACG
ATCAGGCGGAGACCGTGCTGCTGCAACTGCTGCGCGGCGCGGGCATCGCGGGGTTGGCGGCGATGGCGCCGCAGTACCGG
CCGGCGCTTGCCGACGTCGTGCGCATGCGTCCGCTCCTGCATCTGTTGCGCGCGCAGCTCGAGCGCTACGCGCAGCAGCA
TGCGCTGCGCTGGATCGACGACGAATCGAACACGGATACGCGCTATGCGCGCAATGCGCTGCGCGTCGACGTGCTTCCCG
CGCTTGCGCCGCACTTTCCCGGCTTTCGCGATGCGCTCGCCCGCACCGCGCAGCATGCGGCGGCGGCCCAGCGTCTGCTC
GACGATCTCGCCGCGATCGATCTGCGCGCGGTCGCGCGCGCGGACGTGCGCGTGTTGTCGCGCGATGCGCTCGTCGCGCT
CGACGACGAGCGCGGGGCGAACTTGCTGCGTTACTGGATGCGCTCGCTCGGGCTGCCGGGCGCGTCGGCCGCGCGCCTTG
CCGAGATGGTGAAGCAGTTGCGCGCGGCGCGCGATGCGCATGCGTTGCGCGTCGATCATGCGGGTTGGCGCCTGCGTCTT
TATCGCGACGACGTCCAGTGGGAGGCGGGCGACGGTGCCGCATCGGAGGCGGCGCGCGCCGCGCGCGCCGATGTGGCTGA
CGATGATGCGGCCGATGCGCGCGACGACAGGGCCGATGCATCGGCCGCCGCGCGGCTTCCGGCATGCGCGCTCGCCTGGC
GGGGGCACGAGGTGTGGCGCCTGCCCGGCTGGCGCGGCAGCTTCGTGTTCAGTCCGGTCGCGGCGCACGAGCACGATGCG
GTGCCGGAGGCGCTGCTCTCGTCAGCCGCGCTGCGCGCCTGCGCGCGCGCGGGCGGCGAGCGCATGCGCACCCGGCAGGG
CGGCCCCGGGCGCACGCTGAAGAATCTGTTCCAGGAGCGCGGCGTGCCCGCCTGGCAGCGCGATGTGCCGCTGTTGTACG
TCGGCGAGCGGTTGCTGTTCGTGCCGCGCATCGGCGTGAATCGGGCGACGCACGACGGCGCCGATGCGCCGGGCGGCTGG
CGGCGCATCGAATGGCGGCCCGACATGCTGATCGCGTAA

Upstream 100 bases:

>100_bases
CGCTTCGAGCGCTTGATGGCCTACGGCAAGTTCAAGGAAACGACGCCGCGCGCATGAGCGCCGCGCCGTCGAGTCGCGAG
GCGCGTCGCGCCTTCCCGCA

Downstream 100 bases:

>100_bases
GCGCTCCATCCGGCGCGCGTCGCGGCGGCCACGAATCCCGGGCGCGCGGAAAAAGGTCGCGACCCGGCCTGCCGGCGCCG
TCGCGGCTTGTCAAGCAGCC

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALHVHHGLSANADAWLAHCAETA
QALGARFDAARVDVPRASGQGIEASARDARYRALETMCARYGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYR
PALADVVRMRPLLHLLRAQLERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
DDLAAIDLRAVARADVRVLSRDALVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQLRAARDAHALRVDHAGWRLRL
YRDDVQWEAGDGAASEAARAARADVADDDAADARDDRADASAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDA
VPEALLSSAALRACARAGGERMRTRQGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGW
RRIEWRPDMLIA

Sequences:

>Translated_492_residues
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALHVHHGLSANADAWLAHCAETA
QALGARFDAARVDVPRASGQGIEASARDARYRALETMCARYGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYR
PALADVVRMRPLLHLLRAQLERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
DDLAAIDLRAVARADVRVLSRDALVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQLRAARDAHALRVDHAGWRLRL
YRDDVQWEAGDGAASEAARAARADVADDDAADARDDRADASAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDA
VPEALLSSAALRACARAGGERMRTRQGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGW
RRIEWRPDMLIA
>Mature_492_residues
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALHVHHGLSANADAWLAHCAETA
QALGARFDAARVDVPRASGQGIEASARDARYRALETMCARYGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYR
PALADVVRMRPLLHLLRAQLERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
DDLAAIDLRAVARADVRVLSRDALVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQLRAARDAHALRVDHAGWRLRL
YRDDVQWEAGDGAASEAARAARADVADDDAADARDDRADASAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDA
VPEALLSSAALRACARAGGERMRTRQGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGW
RRIEWRPDMLIA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=458, Percent_Identity=34.061135371179, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 53435; Mature: 53435

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWLAHCAETAQALGARFDAARVDVPRASGQGIEASARDARYRALETMCAR
EECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYRPALADVVRMRPLLHLLRAQL
HCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DDLAAIDLRAVARADVRVLSRDALVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RAARDAHALRVDHAGWRLRLYRDDVQWEAGDGAASEAARAARADVADDDAADARDDRADA
HHHHHHHHEEEECCCCEEEEEECCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDAVPEALLSSAALRACARAGGE
HHHHHCCHHHHHCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHCCHH
RMRTRQGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGW
HHHHCCCCCCHHHHHHHHHCCCCCHHHCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCC
RRIEWRPDMLIA
EEEEECCCCCCC
>Mature Secondary Structure
MIPPHEFSAERVVFDALGVALSALPDDTPIAIAYSGGLDSTVLLHAAARIAGAGRCIALH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEE
VHHGLSANADAWLAHCAETAQALGARFDAARVDVPRASGQGIEASARDARYRALETMCAR
EECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
YGARTLWLAQHADDQAETVLLQLLRGAGIAGLAAMAPQYRPALADVVRMRPLLHLLRAQL
HCCCEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERYAQQHALRWIDDESNTDTRYARNALRVDVLPALAPHFPGFRDALARTAQHAAAAQRLL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DDLAAIDLRAVARADVRVLSRDALVALDDERGANLLRYWMRSLGLPGASAARLAEMVKQL
HHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RAARDAHALRVDHAGWRLRLYRDDVQWEAGDGAASEAARAARADVADDDAADARDDRADA
HHHHHHHHEEEECCCCEEEEEECCCCEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SAAARLPACALAWRGHEVWRLPGWRGSFVFSPVAAHEHDAVPEALLSSAALRACARAGGE
HHHHHCCHHHHHCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHCCHH
RMRTRQGGPGRTLKNLFQERGVPAWQRDVPLLYVGERLLFVPRIGVNRATHDGADAPGGW
HHHHCCCCCCHHHHHHHHHCCCCCHHHCCCEEEECCEEEEECCCCCCCCCCCCCCCCCCC
RRIEWRPDMLIA
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA