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The map label for this gene is braE [H]

Identifier: 126454057

GI number: 126454057

Start: 971590

End: 972759

Strand: Direct

Name: braE [H]

Synonym: BURPS1106A_0990

Alternate gene names: 126454057

Gene position: 971590-972759 (Clockwise)

Preceding gene: 126453328

Following gene: 126453383

Centisome position: 24.36

GC content: 66.24

Gene sequence:

>1170_bases
ATGACATCCATTCAACCGATCGAAACATCGGCTTCGCTCGTCGAGGAACGCCACCCCGCGAAGACCGCCACCGTCGGCAT
CCTGATCGCGGCGTTCGTGATCGCCGCGCCGCTCGTCATCGGCGCGGCGGGCGGCAACTACTGGGTCCGCGTGCTCGACT
TCGCGATGCTGTACGTGATGCTCGCGCTCGGCCTGAACGTGGTGGTCGGCTTCGCCGGCCTGCTCGATCTCGGCTACATC
GCGTTCTACGCGGTGGGCGCCTATACCGCGGCGCTGTTGAGCTCGCCGCACCTGACGTCGCACTTCGACTGGATCGCGCA
GATGGTGCCGGGCGGCCTGCACGTGCCGTTCCTGCTGATCGTGCCGATCGCGATGGCGCTCGCGGCGATCTTCGGCATCC
TGCTCGGCGCGCCGACGCTGCGCCTGCGCGGCGATTACCTCGCGATCGTCACGCTCGGCTTCGGCGAGATCGTCCGGATC
TTCATGAACAACCTCGACCGCCCGGTCAACGTGACGAACGGGCCGCAAGGGATCACCGGCATCGATCCCGTCCAGGTCGC
GGGCTTCAACCTGTCGCAGACGCACGAGATCTTCGGCTTCTCGCTGCCGTCCGTGTACATGTACTACTACCTGTTCGTGC
TCTGCGCGCTGCTCGTCATCTGGGTCTGCACGCGCCTGCAGCACTCGCGGATCGGCCGCGCATGGGCCGCGATTCGCGAA
GACGAGATCGCCGCGAAGGCGATGGGCATCAACACCCGCAACGTGAAGCTGCTCGCGTTCGCGATGGGCGCGTCGTTCGG
CGGCCTGTCGGGCGCGATGTTCGGCGCGTTCCAGGGCTTCGTGTCGCCGGAATCGTTCACGTTCTGGGAATCGGTCGTCG
TGCTCGCGTGCGTCGTGCTGGGCGGCATGGGCCACATCCCGGGCGTGATTCTCGGCGCGGTCCTGCTCGCCGTGTTCCCC
GAATTCCTGCGCTCGACGATGAGCCCGCTGCAGCACATGCTGTTCGGCCACGACATCGTCGATACCGAAGTGATTCGCCA
GGCTCTCTACGGCCTCGCGATGGTGGTCATCATGCTGTATCGCTCGGAAGGCCTGTGGCCCGCGCCGAAGCACGAGGATC
GGATCGCGAAACTGGCGAAGCGCGGCGGCAAGAAGCCGGTGCGCGCGTAA

Upstream 100 bases:

>100_bases
CAGGACGTGTTCGCGTTCGTCGTGCTCATCATCGTGCTCGTGTTCCGGCCGTCGGGCCTGCTGGGCGAACGTGTCGCCGA
CCGCGCGTAAGGAGAACAAC

Downstream 100 bases:

>100_bases
AGGACGTGGGACCGGCGCTGCGCGCACGAGCGCGAGCGCCGGTCGACCCAGGAGAAAAGGTAATGAGCGAACAAATTCGA
CTGTCCGTCAAAGGCGTGAA

Product: hydrophobic amino acid ABC transporter permease/ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTSIQPIETSASLVEERHPAKTATVGILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSHFDWIAQMVPGGLHVPFLLIVPIAMALAAIFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNVTNGPQGITGIDPVQVAGFNLSQTHEIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVLGGMGHIPGVILGAVLLAVFP
EFLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDRIAKLAKRGGKKPVRA

Sequences:

>Translated_389_residues
MTSIQPIETSASLVEERHPAKTATVGILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYI
AFYAVGAYTAALLSSPHLTSHFDWIAQMVPGGLHVPFLLIVPIAMALAAIFGILLGAPTLRLRGDYLAIVTLGFGEIVRI
FMNNLDRPVNVTNGPQGITGIDPVQVAGFNLSQTHEIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVLGGMGHIPGVILGAVLLAVFP
EFLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDRIAKLAKRGGKKPVRA
>Mature_388_residues
TSIQPIETSASLVEERHPAKTATVGILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVMLALGLNVVVGFAGLLDLGYIA
FYAVGAYTAALLSSPHLTSHFDWIAQMVPGGLHVPFLLIVPIAMALAAIFGILLGAPTLRLRGDYLAIVTLGFGEIVRIF
MNNLDRPVNVTNGPQGITGIDPVQVAGFNLSQTHEIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRED
EIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVLGGMGHIPGVILGAVLLAVFPE
FLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLYRSEGLWPAPKHEDRIAKLAKRGGKKPVRA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=346, Percent_Identity=42.485549132948, Blast_Score=212, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 41922; Mature: 41791

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSIQPIETSASLVEERHPAKTATVGILIAAFVIAAPLVIGAAGGNYWVRVLDFAMLYVM
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
LALGLNVVVGFAGLLDLGYIAFYAVGAYTAALLSSPHLTSHFDWIAQMVPGGLHVPFLLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHH
VPIAMALAAIFGILLGAPTLRLRGDYLAIVTLGFGEIVRIFMNNLDRPVNVTNGPQGITG
HHHHHHHHHHHHHHHCCCCEEECCCEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
IDPVQVAGFNLSQTHEIFGFSLPSVYMYYYLFVLCALLVIWVCTRLQHSRIGRAWAAIRE
CCCHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEIAAKAMGINTRNVKLLAFAMGASFGGLSGAMFGAFQGFVSPESFTFWESVVVLACVVL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
GGMGHIPGVILGAVLLAVFPEFLRSTMSPLQHMLFGHDIVDTEVIRQALYGLAMVVIMLY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSEGLWPAPKHEDRIAKLAKRGGKKPVRA
HCCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]