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The map label for this gene is gatB [H]

Identifier: 126453954

GI number: 126453954

Start: 173811

End: 175283

Strand: Direct

Name: gatB [H]

Synonym: BURPS1106A_0184

Alternate gene names: 126453954

Gene position: 173811-175283 (Clockwise)

Preceding gene: 126455152

Following gene: 126453377

Centisome position: 4.36

GC content: 65.65

Gene sequence:

>1473_bases
ATGACCCAGTGGGAAGTCGTTATCGGCCTCGAGACGCACGCGCAGCTGTCGACCGTCTCGAAGATCTTCTCGGGCGCGTC
GACGCAGTTCGGCGCGCAACCGAACACGCAGGCGTGCCCCGTCGATCTCGCGCTGCCGGGCGTGCTGCCCGTGCTGAACC
GCGGCGCGGTCGAGCGGGCGATCCGCTTCGGCCTCGCGATCGGCGCGACGGTGGCGCCGCGCAGCGTCTTCGCGCGCAAG
AATTACTTCTATCCGGATCTGCCGAAGGGCTATCAGATCAGCCAGTACGAGATTCCGGTCGTGCAGGGCGGCCAGATCAC
GATCCAGGTGCCCGCCAACGAAAAGGCCGGCAAGCAGGCGTATTCGAAGACGGTCAACCTGACCCGCGCGCACCTCGAGG
AGGACGCGGGCAAGTCGCTGCACGAGGATTTCGCGGGGATGACGGGCATCGACCTGAACCGCGCGGGCACGCCGCTGCTC
GAGATCGTCACCGAGCCGGAAATGCGGAGCGCGGCCGAGGCGGTCGCGTACGCGAAGGCGCTGCACGGCCTCGTCATGTG
GCTCGGCATCTGCGACGGCAACATGCAGGAAGGCTCGTTCCGCTGCGATGCGAACGTGTCGGTGCGCCCGGTCGGCCAGG
AGAAGTTCGGCACGCGCGCCGAGATCAAGAACCTGAACTCGTTCCGCTTTCTCGAAGACGCGATCAACTATGAGGTGCGT
CGCCAGATCGAGCTGATCGAGGACGGCGGCGAAGTCGTCCAGGAAACGCGTCTGTACGACCCGGACAAGCGCGAGACGCG
CTCGATGCGCAGCAAGGAAGACGCGCACGATTACCGCTATTTCCCCGATCCGGACCTGATGCCGCTCGTGATCGGAGCGG
ACTGGATCGCACGCGTGAAGGGCGAGATGCCCGAGCTGCCGGCCGCAATGCAGCAGCGCTTCGTCGAGCAGTATGGCGTG
TCCGCGTACGACGCGGGCGTGCTGACGTCGACGAAGGCGATGGCCGAATATTTCGAGGCGCTGGTCGCGAAGGCGGGCGC
GGCGAATGCGAAGCTCGCCGCGAACTGGCTGATGGGCGACGTATCGTCGCAACTGAACCGCGACGGCATCGATATCGACG
CGTGCCCGGTGTCGGCCGCGCAGCTCGCGCTCGTGCTGCAGCGGATCGCCGACGGCACGATCTCGAACAAGATCGCGAAG
GAAATCTTCGTGACGATCTGGGACGAGAAAGCGGCCGACGAAGGCGCGGCCGATCGCATCATCGAAGCGAAGGGCCTGAA
GCAGATCTCCGATACGGGCGCGCTCGAGGCGATCATCGACGAGGTGCTCGCCGCGAACGCGAAGTCGGTCGAGGAATTCC
GCGCGGGCAAGGACAAGGCGTTCAACGCGCTCGTCGGCCAGGCGATGAAGGCGACGAAGGGCAAGGCGAATCCGCAGCAG
GTCAACGAGCTCCTGAAGAAGAAGCTCGGCTGA

Upstream 100 bases:

>100_bases
TCATCGGCAACTATTTCGACGAAGCCCGGATGCTGCAGGTCGCCGACGCGTTCCAGCGCGCGACCGACTGGCACGTACAA
GAACCGGCAGGAGTGTGAGC

Downstream 100 bases:

>100_bases
GCGGGCGACGCCGGATTGAAGCCGTACCGATGCGAGCGGGCCACCGCCGAACCTGTGTTCGGGGCGGCCCGCTCGCGTTT
CGCGAGGCGCCGGGCCGCGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTQWEVVIGLETHAQLSTVSKIFSGASTQFGAQPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATVAPRSVFARK
NYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQAYSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHGLVMWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIARVKGEMPELPAAMQQRFVEQYGV
SAYDAGVLTSTKAMAEYFEALVAKAGAANAKLAANWLMGDVSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAK
EIFVTIWDEKAADEGAADRIIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATKGKANPQQ
VNELLKKKLG

Sequences:

>Translated_490_residues
MTQWEVVIGLETHAQLSTVSKIFSGASTQFGAQPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATVAPRSVFARK
NYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQAYSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKALHGLVMWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIARVKGEMPELPAAMQQRFVEQYGV
SAYDAGVLTSTKAMAEYFEALVAKAGAANAKLAANWLMGDVSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAK
EIFVTIWDEKAADEGAADRIIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATKGKANPQQ
VNELLKKKLG
>Mature_489_residues
TQWEVVIGLETHAQLSTVSKIFSGASTQFGAQPNTQACPVDLALPGVLPVLNRGAVERAIRFGLAIGATVAPRSVFARKN
YFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQAYSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLE
IVTEPEMRSAAEAVAYAKALHGLVMWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVRR
QIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIARVKGEMPELPAAMQQRFVEQYGVS
AYDAGVLTSTKAMAEYFEALVAKAGAANAKLAANWLMGDVSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAKE
IFVTIWDEKAADEGAADRIIEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATKGKANPQQV
NELLKKKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=39.2, Blast_Score=357, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI115534094, Length=472, Percent_Identity=32.4152542372881, Blast_Score=229, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.7399617590822, Blast_Score=231, Evalue=3e-61,
Organism=Drosophila melanogaster, GI45550808, Length=497, Percent_Identity=36.2173038229376, Blast_Score=338, Evalue=7e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53323; Mature: 53192

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQWEVVIGLETHAQLSTVSKIFSGASTQFGAQPNTQACPVDLALPGVLPVLNRGAVERA
CCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHHH
IRFGLAIGATVAPRSVFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQA
HHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCHHHHHH
YSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKA
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LHGLVMWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
HHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHHHCHHHH
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIARVK
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHC
GEMPELPAAMQQRFVEQYGVSAYDAGVLTSTKAMAEYFEALVAKAGAANAKLAANWLMGD
CCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAKEIFVTIWDEKAADEGAADRI
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCCCCHHHHH
IEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATKGKANPQQ
HHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
VNELLKKKLG
HHHHHHHHCC
>Mature Secondary Structure 
TQWEVVIGLETHAQLSTVSKIFSGASTQFGAQPNTQACPVDLALPGVLPVLNRGAVERA
CCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCHHHHHH
IRFGLAIGATVAPRSVFARKNYFYPDLPKGYQISQYEIPVVQGGQITIQVPANEKAGKQA
HHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCHHHHHH
YSKTVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAKA
HHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHH
LHGLVMWLGICDGNMQEGSFRCDANVSVRPVGQEKFGTRAEIKNLNSFRFLEDAINYEVR
HHHHHHHHHHCCCCCCCCCEEECCCCEEEECCHHHCCCHHHHHCCCHHHHHHHHHCHHHH
RQIELIEDGGEVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIGADWIARVK
HHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHC
GEMPELPAAMQQRFVEQYGVSAYDAGVLTSTKAMAEYFEALVAKAGAANAKLAANWLMGD
CCCCCCCHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VSSQLNRDGIDIDACPVSAAQLALVLQRIADGTISNKIAKEIFVTIWDEKAADEGAADRI
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCCCCCCCCHHHHH
IEAKGLKQISDTGALEAIIDEVLAANAKSVEEFRAGKDKAFNALVGQAMKATKGKANPQQ
HHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
VNELLKKKLG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA