Click here to switch to the map view.

The map label for this gene is ligA [H]

Identifier: 126453906

GI number: 126453906

Start: 2452649

End: 2454724

Strand: Reverse

Name: ligA [H]

Synonym: BURPS1106A_2499

Alternate gene names: 126453906

Gene position: 2454724-2452649 (Counterclockwise)

Preceding gene: 126452604

Following gene: 126452569

Centisome position: 61.55

GC content: 68.4

Gene sequence:

>2076_bases
ATGGCCCGATCCCCAGTTGAACCGCCCGCCAGCCAGCCGGCGAAGCGCGCCGCGTGGCTGCGCGCCGAGCTCGAGCGGGC
GAACTACGCGTATTACGTGCTCGACCAGCCGGACCTGCCCGACGCCGAGTACGATCGCCTCTTCGTCGAACTCCAACGGA
TCGAGGCCGAGCACCCCGATCTCGTCACGCCCGATTCGCCGACGCAGCGCGTCGGCGGCGAGGCCGCGAGCGGCTTCACG
CCGGTCGTCCACGACAAGCCGATGCTCTCGCTCAACAACGGCTTTGCCGACGAGGACGTCATCGCGTTCGACAAGCGCGT
CGCCGACGGCCTCGACAAGGCGACCGACCTCGCCGGCACCGTGACCGAGCCCGTCGAATACGCGTGCGAGCTGAAGTTCG
ACGGCCTCGCGATCTCGCTGCGCTACGAGAACGGCCGCTTCGTGCAGGCGTCGACGCGCGGCGACGGCACGACGGGCGAA
GACGTCACCGAGAATATCCGCACGATCCGTGCGATTCCGCTGACGCTCAAGGGCAAGCGGATACCGCGCATGCTCGACGT
GCGCGGCGAGGTGCTGATGTTCAAGCGCGATTTCGCGCGGCTGAACGAGCGCCAGCGCGCGGCGGGCCAGCGCGAGTTCG
CCAATCCGCGCAACGCGGCGGCGGGCAGCCTGCGGCAGCTCGATTCGAAGATCACCGCGTCGCGGCCGCTTTCGTTTTTC
GCATACGGAATCGGCGTGCTCGACGGCGCCGACATGCCCGACACGCACAGCGGCTTGCTCGACTGGTACGAGACGCTCGG
GTTGCCCGTGAACCGGGAGCGCGCGGTCGTGCGCGGCGCGGCGGGCCTGCTCGCGTTCTTCCATTCGGTGGGCGAGCGGC
GCGAATCGTTGCCGTACGACATCGACGGCGTCGTCTACAAGGTGAACCGCCGCGACGAGCAGGATCGGCTCGGCTTCGTG
TCGCGCGCGCCGCGCTTCGCGCTCGCGCACAAGTTCCCCGCGCAGGAGGCGCTCACGAAGCTCATCGCGATCGACGTGCA
GGTCGGCCGCACCGGCGCGATCACGCCGGTCGCGCGCCTCGAGCCCGTGTTCGTCGGCGGCGCGACCGTCACGAACGCGA
CGCTGCACAACGAGGACGAGGTGCGCCGCAAGGACATCCGCATCGGCGACACGGTCATCGTGCGCCGCGCGGGCGACGTG
ATTCCCGAAGTGGTATCGGCCGTGCTCGACCGGCGTCCCGCCGACGCGCAGGAGTTCGTGATGCCGACCGAATGCCCGGA
ATGCGGATCGCGGATCGAGCGATTGCCCGACGAGGCGATCGCGCGCTGCACGGGCGGGCTGTTTTGCCCCGCGCAGCGCA
AGCAGGCGCTCTGGCACTTCGCGCAGCGCCGTGCGCTCGACATCGATGGGCTCGGCGAGAAGATCATCGATCAACTCGTC
GAGCAGAACCTCGTGCGCACGCCGGCCGATCTGTTCAATCTCGGCTTCTCGACGCTCGTCGCGCTCGATCGCTTCGCGGA
GAAATCCGCGCGGAATCTGATCGATTCGCTCGAAAAGGCGAAGCACACGACGCTCGCGCGCTTCATTTACGCGCTCGGCA
TCCGGCACGTCGGCGAATCGACGGCGAAGGACCTCGCGAAGCACTTCGGCTCGCTCGATCCGATCATGGACGCGCCGATC
GACGCATTGCTCGAGGTCAACGACGTCGGGCCGATCGTCGCCGAATCGATCCATCAATTCTTCGCCGAGGAGCACAATCG
CACGGTGATCGAACAGTTGCGCGCGCGGGGCAAGGTCACATGGCCCGAAGGGCCGCCCGCGCCGCGCGCGCCGCAAGGCG
TGCTCGCGGGCAAGACCGTCGTGCTCACGGGCACGCTGCCGACGCTCACGCGGGAAGCCGCGAAGGAGATGCTCGAGGCG
GCGGGCGCGAAAGTCGCGGGCTCGGTATCGAAGAAGACCGATTACGTCGTCGCGGGCGCCGACGCGGGCAGCAAGCTCGC
GAAAGCCGAGGAACTCGGCATTCCGGTGCTGGACGAAGCAGGCATGCACACACTTCTGGAGGGCCACGCGCGATGA

Upstream 100 bases:

>100_bases
CCGCGATTTCCACGCTCGAGCGGCCATTCAGCCGATGCCGAAAAGGGCTCGAACTGAGATAATCGTCGCCTGATTATCCT
CACGAGAAAGTGCCGCCAGC

Downstream 100 bases:

>100_bases
TTCGCGAGATTTTGAAGATGGGCGATCCACGTCTGCTGGAAGTCGCCCGGCCGGTGGAGGCGTTCAACACGCCGGAGCTG
CACGCGCTCGTCGCGGACAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MARSPVEPPASQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFVELQRIEAEHPDLVTPDSPTQRVGGEAASGFT
PVVHDKPMLSLNNGFADEDVIAFDKRVADGLDKATDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGE
DVTENIRTIRAIPLTLKGKRIPRMLDVRGEVLMFKRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
AYGIGVLDGADMPDTHSGLLDWYETLGLPVNRERAVVRGAAGLLAFFHSVGERRESLPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVSAVLDRRPADAQEFVMPTECPECGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
EQNLVRTPADLFNLGFSTLVALDRFAEKSARNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDAPI
DALLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRARGKVTWPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEA
AGAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHTLLEGHAR

Sequences:

>Translated_691_residues
MARSPVEPPASQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFVELQRIEAEHPDLVTPDSPTQRVGGEAASGFT
PVVHDKPMLSLNNGFADEDVIAFDKRVADGLDKATDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGE
DVTENIRTIRAIPLTLKGKRIPRMLDVRGEVLMFKRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
AYGIGVLDGADMPDTHSGLLDWYETLGLPVNRERAVVRGAAGLLAFFHSVGERRESLPYDIDGVVYKVNRRDEQDRLGFV
SRAPRFALAHKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVSAVLDRRPADAQEFVMPTECPECGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
EQNLVRTPADLFNLGFSTLVALDRFAEKSARNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDAPI
DALLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRARGKVTWPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEA
AGAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHTLLEGHAR
>Mature_690_residues
ARSPVEPPASQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFVELQRIEAEHPDLVTPDSPTQRVGGEAASGFTP
VVHDKPMLSLNNGFADEDVIAFDKRVADGLDKATDLAGTVTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGED
VTENIRTIRAIPLTLKGKRIPRMLDVRGEVLMFKRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFFA
YGIGVLDGADMPDTHSGLLDWYETLGLPVNRERAVVRGAAGLLAFFHSVGERRESLPYDIDGVVYKVNRRDEQDRLGFVS
RAPRFALAHKFPAQEALTKLIAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVI
PEVVSAVLDRRPADAQEFVMPTECPECGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVE
QNLVRTPADLFNLGFSTLVALDRFAEKSARNLIDSLEKAKHTTLARFIYALGIRHVGESTAKDLAKHFGSLDPIMDAPID
ALLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRARGKVTWPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEAA
GAKVAGSVSKKTDYVVAGADAGSKLAKAEELGIPVLDEAGMHTLLEGHAR

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=42.8571428571429, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=57.6980568011958, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=405, Percent_Identity=25.1851851851852, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324791, Length=90, Percent_Identity=42.2222222222222, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75597; Mature: 75466

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSPVEPPASQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFVELQRIEAEHPD
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LVTPDSPTQRVGGEAASGFTPVVHDKPMLSLNNGFADEDVIAFDKRVADGLDKATDLAGT
CCCCCCCHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGEDVTENIRTIRAIPLTLKGKR
HHHHHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHEEECEEECCCC
IPRMLDVRGEVLMFKRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
CCHHHHCCCCEEEEHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
AYGIGVLDGADMPDTHSGLLDWYETLGLPVNRERAVVRGAAGLLAFFHSVGERRESLPYD
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEALTKLIAIDVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVSAVLDRRPADAQEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPECGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EQNLVRTPADLFNLGFSTLVALDRFAEKSARNLIDSLEKAKHTTLARFIYALGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLDPIMDAPIDALLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRARGKVT
HHHHHHHHCCCCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
WPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
DAGSKLAKAEELGIPVLDEAGMHTLLEGHAR
CCCCHHHHHHHCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
ARSPVEPPASQPAKRAAWLRAELERANYAYYVLDQPDLPDAEYDRLFVELQRIEAEHPD
CCCCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
LVTPDSPTQRVGGEAASGFTPVVHDKPMLSLNNGFADEDVIAFDKRVADGLDKATDLAGT
CCCCCCCHHHCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
VTEPVEYACELKFDGLAISLRYENGRFVQASTRGDGTTGEDVTENIRTIRAIPLTLKGKR
HHHHHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHEEECEEECCCC
IPRMLDVRGEVLMFKRDFARLNERQRAAGQREFANPRNAAAGSLRQLDSKITASRPLSFF
CCHHHHCCCCEEEEHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
AYGIGVLDGADMPDTHSGLLDWYETLGLPVNRERAVVRGAAGLLAFFHSVGERRESLPYD
HHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQDRLGFVSRAPRFALAHKFPAQEALTKLIAIDVQVGRTGAITPVARL
CCCEEEEECCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVSAVLDRRPADAQEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPECGSRIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
EQNLVRTPADLFNLGFSTLVALDRFAEKSARNLIDSLEKAKHTTLARFIYALGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLDPIMDAPIDALLEVNDVGPIVAESIHQFFAEEHNRTVIEQLRARGKVT
HHHHHHHHCCCCCCHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEE
WPEGPPAPRAPQGVLAGKTVVLTGTLPTLTREAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
DAGSKLAKAEELGIPVLDEAGMHTLLEGHAR
CCCCHHHHHHHCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA