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The map label for this gene is gshA

Identifier: 126453851

GI number: 126453851

Start: 455796

End: 457085

Strand: Direct

Name: gshA

Synonym: BURPS1106A_0489

Alternate gene names: NA

Gene position: 455796-457085 (Clockwise)

Preceding gene: 126452066

Following gene: 126451986

Centisome position: 11.43

GC content: 65.66

Gene sequence:

>1290_bases
ATGGTTCCCCATCTCGTTACGGCGTTAAACGGCCCGCTGCTCGAGCTCGAGCGGAAGATCCTCGACGCCACGCCTGCGAT
CGAACGCTGGTTCAGGCTCGAATGGCAGGAACACACGCCGCCGTTCTACTGTTCGGTCGATCTGCGCAACGCGGGCTTCA
AGCTCGCGCCCGTCGACGCGAACCTGTTTCCGGGCGCGTTCAACAACCTGCCGCCCGAAGTGCTGCCGCTCGCCGTGCAG
GCGGCGATGGCCGCGATCGAGAAGATCTGCCCGGACGCGAAGAATCTGCTCGTGATTCCCGAGCTGCCGACGCGCAATGC
GTTCTATCTCGAGAACGTCGCGCGCCTCGCGACGATCATGCGTCAGGCCGGCCTGAACGTGCGCTTCGGTTCGCTCGATC
CGAGCATCGCCGAGGTCACGCCGATCACGCTCGCCGACGGCCAGAAGATCGTGCTCGAGCCGCTCGAGCGCACGCCGCGT
CGCCTCGGCCTGAAGAATTTCGATCCGTGCTCGATCCTGCTGAACAACGATCTGTCGGCGGGCATCCCGAGCGTGCTGGA
AAACCTGCACGAGCAGTATCTGCTGCCGCCGCTGCATGCCGGCTGGGCGGTGCGCCGCAAGTCGACGCACTTCTCGTGCT
ATGACGACGTCGCGAAGAAATTCGCGAAGATGGTGGGTGTCGATCCCTGGATGCTGAATCCGTATTTCTCGCCTGTCGAA
GGTGTCGACTGGCACGCGAGCGACGGCGGCGAGGCGCTCGCCGAAGCGATCGACGCAGTGCTCAAGAAGATCGCGCGCAA
GTACCGCGAATACGGGATCGGCGAGAAGCCGTACGTCGTCGTGAAGGCGGACGCCGGCACGGCGGGGCGCGGCGTGATGA
CGGTGCACGATGCGTCCGAGATCGGCAGGATGACGAAGCACGAGCGCGCGCAGATGGCCGAATCGAAGGCGGGCGTGCCG
GTGAGCGACGTGATCGTGCAGGAAGGCGTCTATACGTTCGAGCGCATCGGCGACGAGGTGGCCGAGCCCGTCGTCTACAT
GATCGACCGCTACGTGGTGGGCGGGTTCTACCGCACACACGGCGCGCGCGAGCGCGACCAGAATCTGAACGCGCCCGGCA
TGCATTACGTGCCGCTCGGCTTCGAGCACACGGCGCTGCCGGACACGCACGCGAAGCCTGGCGCGGCGCCGCCGAACCGC
TTCTACATGTATGGCGTCGTCGCACGGCTCGGGCTGCTCGCGTCGTCGGTCGAGCTCGAGAAGACCGACCCGGAAGCGAT
CCAGGTGTGA

Upstream 100 bases:

>100_bases
AGAGACAATCATGCGGTAAATCCTTGCGATACCGGGAACAATCCCCAAATGCGACAGGCAATTGCTCTACAATCTTTTTT
CTCCTGTCCGCGAGTCACAC

Downstream 100 bases:

>100_bases
CGCATTCCCTTTGGCATCAGGATTCGCATGGACATTCTCTTTATCGCCGACCCGCTCGATCGCTTCAAGATCTACAAGGA
CTCGACCTACGCGATGATGG

Product: glutamate--cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPPEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPSIAEVTPITLADGQKIVLEPLERTPR
RLGLKNFDPCSILLNNDLSAGIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSPVE
GVDWHASDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASEIGRMTKHERAQMAESKAGVP
VSDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTHGARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNR
FYMYGVVARLGLLASSVELEKTDPEAIQV

Sequences:

>Translated_429_residues
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPPEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPSIAEVTPITLADGQKIVLEPLERTPR
RLGLKNFDPCSILLNNDLSAGIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSPVE
GVDWHASDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASEIGRMTKHERAQMAESKAGVP
VSDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTHGARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNR
FYMYGVVARLGLLASSVELEKTDPEAIQV
>Mature_429_residues
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDANLFPGAFNNLPPEVLPLAVQ
AAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIMRQAGLNVRFGSLDPSIAEVTPITLADGQKIVLEPLERTPR
RLGLKNFDPCSILLNNDLSAGIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSPVE
GVDWHASDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASEIGRMTKHERAQMAESKAGVP
VSDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTHGARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNR
FYMYGVVARLGLLASSVELEKTDPEAIQV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47335; Mature: 47335

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDA
CCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPPEVLPLAVQAAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIM
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGSLDPSIAEVTPITLADGQKIVLEPLERTPRRLGLKNFDPCSILLNNDLSA
HHCCCCEEECCCCCCHHHCCEEEECCCCEEECCHHHHCHHHCCCCCCCCEEEEECCCCCC
GIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSPVE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCC
GVDWHASDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECHHH
IGRMTKHERAQMAESKAGVPVSDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNRFYMYGVVARLGLLASSVELE
CCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KTDPEAIQV
CCCCHHCCC
>Mature Secondary Structure
MVPHLVTALNGPLLELERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDA
CCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPPEVLPLAVQAAMAAIEKICPDAKNLLVIPELPTRNAFYLENVARLATIM
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGSLDPSIAEVTPITLADGQKIVLEPLERTPRRLGLKNFDPCSILLNNDLSA
HHCCCCEEECCCCCCHHHCCEEEECCCCEEECCHHHHCHHHCCCCCCCCEEEEECCCCCC
GIPSVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVGVDPWMLNPYFSPVE
CHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCC
GVDWHASDGGEALAEAIDAVLKKIARKYREYGIGEKPYVVVKADAGTAGRGVMTVHDASE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECHHH
IGRMTKHERAQMAESKAGVPVSDVIVQEGVYTFERIGDEVAEPVVYMIDRYVVGGFYRTH
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHC
GARERDQNLNAPGMHYVPLGFEHTALPDTHAKPGAAPPNRFYMYGVVARLGLLASSVELE
CCCHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
KTDPEAIQV
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA