Click here to switch to the map view.

The map label for this gene is rlmN

Identifier: 126453816

GI number: 126453816

Start: 2219418

End: 2220554

Strand: Reverse

Name: rlmN

Synonym: BURPS1106A_2230

Alternate gene names: 126453816

Gene position: 2220554-2219418 (Counterclockwise)

Preceding gene: 126452672

Following gene: 126454923

Centisome position: 55.67

GC content: 65.35

Gene sequence:

>1137_bases
ATGGCGGGCGAAACCATCGTCAATCTACTCGACCTCGACGCCGAGGGCCTCGTCGCGTACTGCGGCAGCCTCGGCGAGAA
GGCGTTTCGCGCTAAGCAGCTGCAGCGCTGGATCCACCAATACAACGCAGCCGATTTCGACGGGATGACCGATCTCGCGA
AATCGCTGCGCGAGAAGCTGAAGGGGCGCGCCGTGATCGGCACGCCCGATATCCTGAGCGATCACGTGTCGGCCGACGGC
ACGCGCAAGTGGCTCATCAACGTCGGCAACGGCAATGCGGTCGAGACGGTGTTCATCCCCGAGGAGACGCGCGGCACGCT
GTGCGTGTCGTCGCAGGCGGGGTGCGCGGTCAATTGCCGCTTCTGCTCGACCGGCAAGCAGGGTTTTTCCCGCAATCTGT
CGACGGGCGAGATCGTCGGGCAACTGCGGATGGCGGAATTCGCGCTGCGCGCGTCGCTGGGCCGTGCGCCCGGCCCCAAC
GGCAAGGCCGAGCGGGTGATCACGAACGTCGTGATGATGGGCATGGGCGAGCCGCTTCTCAATTACTCGGCGGTCGTGCC
CGCGATGCGTCTCATGCTCGACGACAACGCGTATGGCCTGTCGCGCCGGCGCGTGACACTGTCGACGTCGGGTGTCGTGC
CGATGATGGACCGGCTCGGCGCGGAGCTGCCCGTCGCGCTCGCGGTGTCGCTGCATGCGCCGAACGATGCGCTGCGCGAC
GAACTCGTGCCGCTCAACAAGAAGCATCCGCTGCGTGAGCTGATGGCCGCGTGCCAGCGTTACCTGAAGGTCGCGCCGCG
CGATTTCATCACGTTCGAGTACTGCATGCTCGACGGCGTCAACGATACCGAAGCGCACGCACGCGAGTTGCTCGCGGTAA
CGCGCGACGTGCCGTGCAAATTCAATCTGATTCCGTTCAATCCATTCCCGGAATCGGGGCTCGTGCGCTCGAAGACGGAG
CAGATCAAGCGCTTCGCGCAGGTTCTGATCGACGCAGGCGTCGTGACGACGATCCGCAAGACGCGCGGCGACGATATCGA
CGCCGCGTGCGGTCAACTGGCGGGCGCGGTCAAGGATCGCACGCGTCTTGCCGAGCGCACCGGCGCGTCGAAGATTATCG
AGGTCCGGGCGGTTTGA

Upstream 100 bases:

>100_bases
AAACGGCGCGCGCCGAAGTCGCGTTCTTCTTCCCGGAAATGAACGTCTACTCGCGCTAAGCGAGTCGGGGTCCGGCGCGT
TCGGCGGCAAGGCAGGCATC

Downstream 100 bases:

>100_bases
CCGGGGTGGCCGGGCTGACAGGGAAATACGCGTAAAGCGATCGGGGGCGGCGCGCGGCGCCGCGCAGTGTGTGACAACGG
TGGTGCTGTGAGCCCGGCCG

Product: radical SAM protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MAGETIVNLLDLDAEGLVAYCGSLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGTPDILSDHVSADG
TRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPN
GKAERVITNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLVRSKTE
QIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDRTRLAERTGASKIIEVRAV

Sequences:

>Translated_378_residues
MAGETIVNLLDLDAEGLVAYCGSLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGTPDILSDHVSADG
TRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPN
GKAERVITNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLVRSKTE
QIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDRTRLAERTGASKIIEVRAV
>Mature_377_residues
AGETIVNLLDLDAEGLVAYCGSLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGTPDILSDHVSADGT
RKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPNG
KAERVITNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRDE
LVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCKFNLIPFNPFPESGLVRSKTEQ
IKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDRTRLAERTGASKIIEVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=375, Percent_Identity=53.6, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_BURM7 (A3MK77)

Other databases:

- EMBL:   CP000548
- RefSeq:   YP_001080663.1
- ProteinModelPortal:   A3MK77
- GeneID:   4892410
- GenomeReviews:   CP000548_GR
- KEGG:   bmn:BMA10247_1109
- TIGR:   BMA10247_1109
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK896874
- BioCyc:   BMAL320389:BMA10247_1109-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR013785
- InterPro:   IPR004383
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: NA

EC number: =2.1.1.192

Molecular weight: Translated: 41132; Mature: 41001

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGETIVNLLDLDAEGLVAYCGSLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRAVIGTPDILSDHVSADGTRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCEEECCCHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPNGKAERVITNVVMMGMGEPLL
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLVRSKTEQIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGASKIIEVRAV
HHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
AGETIVNLLDLDAEGLVAYCGSLGEKAFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRAVIGTPDILSDHVSADGTRKWLINVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCEEECCCHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTGEIVGQLRMAEFALRASLGRAPGPNGKAERVITNVVMMGMGEPLL
EECCCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
ELVPLNKKHPLRELMAACQRYLKVAPRDFITFEYCMLDGVNDTEAHARELLAVTRDVPCK
HCCCCCCCCCHHHHHHHHHHHHHCCCCHHEEEEEHEECCCCCHHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLVRSKTEQIKRFAQVLIDAGVVTTIRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLAERTGASKIIEVRAV
HHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA