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The map label for this gene is ntrB [H]

Identifier: 126453742

GI number: 126453742

Start: 2655687

End: 2656829

Strand: Reverse

Name: ntrB [H]

Synonym: BURPS1106A_2689

Alternate gene names: 126453742

Gene position: 2656829-2655687 (Counterclockwise)

Preceding gene: 126453116

Following gene: 126455410

Centisome position: 66.61

GC content: 66.75

Gene sequence:

>1143_bases
ATGGTTCTGAAGAATCTGATCAAGGCCAGGGCCGGCCACCCGGAGCGCTTGTCCGACGATGACCGGCTCGTGCGCTCGGG
ACTCCTGACGGGGCTCGAGGCGTTGCCGACGGTCGTGCTCGTGCTCGATCGGCGCACGCTCAGGATCGCGTTCGCCAATC
CGTCGGCGGAGGCGATGCTCGACATCTCGCGCCGGCAGTTGTCGCAGATGCCGTGGGGCGAGATCTTCCCGAACGCGAGC
GAGCTCGCGACGACGATCACCGCGATCGGCGAGGAGCGCTTTCACGCGACCCATCTGGACACCGTGCTCGATCGTCCGGG
CCGCGAGCCGCTGCACGTGCATGCGATCGTCGGCTTTCTCGAGGGCGCGCCCGAGTTCGTGCTCGTCGAGCTGTTCGAGA
ACGAGCGGCAGTCGCGCACCGACCGCGAGGAGCGGATCCACGATCTCACCGCCGTCAACAAGCAACTGATCCGCAACCTC
GCGCATGAGATCAAGAACCCGCTCGGCGGGATTCGCGGCGCGGCGCAATTGCTCGAGTTCGAGCTGGGCGAGCGCGAGCG
CGACGAGCTGCGCGAGTACACGCAGGTGATCATCAAGGAATCGGACCGGCTGCAGACGCTCGTCGACCGGCTGCTCGAGC
CGCATCGCCATCCGCACATCGTCGGCGACGTGAACATCCACGAAGTGTGCGAGCGGGTGCGCGCGGTGATGCTCGCGGAA
TTCCCGCGCGGCCTCACGATCGAGCGCGATTACGACGTGAGCGTGCCCGATCTGCGCGGCGACAAGGAGCAACTGATCCA
GGCGCTCCTCAATATCGTGCGCAACGCGGCGCAGGCGCTGCGCGAGCGGATCGCGCAGGGCGACGCGCGGATCGAGCTGC
GCACGCGCGTCGCGCGCAAGGTGACGATCGCGAAGCGCCTGTACAGGCTGGCACTGGATTTGCGTGTGATCGACAACGGC
CCCGGCATTCCCGACGACATCCGCGACCGGATCTTCTATCCGCTCGTATCCGGCCGCGACGACGGCAGCGGGCTCGGGCT
CACGCTCGCGCAGACCTTCATCCAGCAGCACGACGGGATGATCGAAGTGGACAGCCGGCCGGGCCGGACCGAATTTCAGA
TTTTGCTGCCGCTCGACCATTGA

Upstream 100 bases:

>100_bases
ACTGTGCGCGAAGGCATCGGACCTTGAAAGGGGACGGCGGGACGCCGTCCCTTTTTTGTTCCGCCCGCGACGGCGGTTTT
TGACACGGCACCCACGCAAG

Downstream 100 bases:

>100_bases
GCGGCGGCGCAGCGAATCTCCATACCTCTGACCGACTATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCGCT
GGGTGCTCGAGAAGGCGCTC

Product: nitrogen regulation protein NR(II)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAMLDISRRQLSQMPWGEIFPNAS
ELATTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLEGAPEFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARKVTIAKRLYRLALDLRVIDNG
PGIPDDIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH

Sequences:

>Translated_380_residues
MVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAMLDISRRQLSQMPWGEIFPNAS
ELATTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLEGAPEFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARKVTIAKRLYRLALDLRVIDNG
PGIPDDIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH
>Mature_380_residues
MVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAMLDISRRQLSQMPWGEIFPNAS
ELATTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLEGAPEFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARKVTIAKRLYRLALDLRVIDNG
PGIPDDIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGMIEVDSRPGRTEFQILLPLDH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=42.1652421652422, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1788549, Length=354, Percent_Identity=28.2485875706215, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790436, Length=259, Percent_Identity=27.7992277992278, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=26.3374485596708, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43137; Mature: 43137

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAML
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
DISRRQLSQMPWGEIFPNASELATTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFL
HHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EGAPEFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VTIAKRLYRLALDLRVIDNGPGIPDDIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGM
HHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCE
IEVDSRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVLKNLIKARAGHPERLSDDDRLVRSGLLTGLEALPTVVLVLDRRTLRIAFANPSAEAML
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
DISRRQLSQMPWGEIFPNASELATTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFL
HHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
EGAPEFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERDELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLTIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDARIELRTRVARK
CCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VTIAKRLYRLALDLRVIDNGPGIPDDIRDRIFYPLVSGRDDGSGLGLTLAQTFIQQHDGM
HHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCE
IEVDSRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]