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The map label for this gene is cyoE [H]

Identifier: 126453695

GI number: 126453695

Start: 479714

End: 480616

Strand: Direct

Name: cyoE [H]

Synonym: BURPS1106A_0517

Alternate gene names: 126453695

Gene position: 479714-480616 (Clockwise)

Preceding gene: 126454556

Following gene: 126454294

Centisome position: 12.03

GC content: 66.33

Gene sequence:

>903_bases
ATGGACACGACACTCTCCCATACGCCCGGTAGCCGTCTCTCCCAGTATCTGGCGCTGACGAAGCCGCGCGTCACGCAGCT
CGCCGTGTTCTGCGCGGTGATCGGGATGTTCCTCGCGACGCCGGGCATGGTGCCGTGGAAAGTGCTGCTCGGCGGCACGA
TCGGCATCGGGCTGCTCGCGGGCTCGGCGTTCGCGATCAATTGCCTCGTCGAGCAGAAGATCGACGCGATGATGCGGCGC
ACCGCGTGGCGTCCGTCCGCGCGCGGCGAGATCACGACCCTGCAGATCCTCGCGTTCTCGACCGTGCTGGGCGGCCTCGG
CGCGTGGACGCTCTACACGTTCACGAATCCGCTGACGATGTGGCTGACGATCGCGACGTTCGTCGGCTACGCGGTGATCT
ACACGCTCCTGCTCAAGCCGATGACGCCGCAGAACATCGTGATCGGCGGCGCGTCCGGCGCGATGCCGCCCGCGCTCGGC
TGGGCGGCCGTCACGGGCGCGGTGCCCGGCGACGCGTGGATTCTCGTGCTGATCATCTTCGTGTGGACGCCGCCGCACTT
CTGGGTGCTCGCGCTCTACCGCCGCAAGGACTACGAGAACGCCGGCCTGCCGATGCTGCCCGTCACGCACGGCGAGCAGT
TCACGCGGCTGCACATCCTGCTGTACACGGTGATCCTGTTCGCCGTCACGATGATGCCGTTCATCTCCGGGATGAGCGGG
GCCGTCTATCTGACGAGCGCGGTGCTGCTCGGCGCGCTCTTCCTCGCGTATGCGTGGAAGATCTACCGCGATTATTCGGA
TGCGCTCGCGCGCCGCGCGTTCCGCTATTCGATCGTCTATCTGTCGCTGCTGTTCGCGGCGCTTTTGGTCGACCACTACG
CACGTCCCGTGATCGGGATGTAA

Upstream 100 bases:

>100_bases
GCTGCTCGTCGTCATGCTAAACTTTCGCATCCTTTCAAGCCGTCCCGGCCGCGTCGCGCAACCCGCGCGCGACGCCGCGC
CCGCGTGACGTTGACCGTAC

Downstream 100 bases:

>100_bases
CTCCAACGGTTCGATTCCGATGCTCAAAACCCTGTTTGCACGCCGCCGCGCGCAGCGCGGCTGGCTGTTCGCCTGCGCGC
TCGCGGGCGCGATGCTGATT

Product: protoheme IX farnesyltransferase

Products: heme O; pyrophosphate [C]

Alternate protein names: Heme B farnesyltransferase; Heme O synthase [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDTTLSHTPGSRLSQYLALTKPRVTQLAVFCAVIGMFLATPGMVPWKVLLGGTIGIGLLAGSAFAINCLVEQKIDAMMRR
TAWRPSARGEITTLQILAFSTVLGGLGAWTLYTFTNPLTMWLTIATFVGYAVIYTLLLKPMTPQNIVIGGASGAMPPALG
WAAVTGAVPGDAWILVLIIFVWTPPHFWVLALYRRKDYENAGLPMLPVTHGEQFTRLHILLYTVILFAVTMMPFISGMSG
AVYLTSAVLLGALFLAYAWKIYRDYSDALARRAFRYSIVYLSLLFAALLVDHYARPVIGM

Sequences:

>Translated_300_residues
MDTTLSHTPGSRLSQYLALTKPRVTQLAVFCAVIGMFLATPGMVPWKVLLGGTIGIGLLAGSAFAINCLVEQKIDAMMRR
TAWRPSARGEITTLQILAFSTVLGGLGAWTLYTFTNPLTMWLTIATFVGYAVIYTLLLKPMTPQNIVIGGASGAMPPALG
WAAVTGAVPGDAWILVLIIFVWTPPHFWVLALYRRKDYENAGLPMLPVTHGEQFTRLHILLYTVILFAVTMMPFISGMSG
AVYLTSAVLLGALFLAYAWKIYRDYSDALARRAFRYSIVYLSLLFAALLVDHYARPVIGM
>Mature_300_residues
MDTTLSHTPGSRLSQYLALTKPRVTQLAVFCAVIGMFLATPGMVPWKVLLGGTIGIGLLAGSAFAINCLVEQKIDAMMRR
TAWRPSARGEITTLQILAFSTVLGGLGAWTLYTFTNPLTMWLTIATFVGYAVIYTLLLKPMTPQNIVIGGASGAMPPALG
WAAVTGAVPGDAWILVLIIFVWTPPHFWVLALYRRKDYENAGLPMLPVTHGEQFTRLHILLYTVILFAVTMMPFISGMSG
AVYLTSAVLLGALFLAYAWKIYRDYSDALARRAFRYSIVYLSLLFAALLVDHYARPVIGM

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group [H]

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily [H]

Homologues:

Organism=Homo sapiens, GI17921982, Length=192, Percent_Identity=36.4583333333333, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1786631, Length=280, Percent_Identity=32.8571428571429, Blast_Score=150, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71998235, Length=286, Percent_Identity=30.4195804195804, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325085, Length=247, Percent_Identity=31.9838056680162, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921064, Length=292, Percent_Identity=30.4794520547945, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006369
- InterPro:   IPR000537 [H]

Pfam domain/function: PF01040 UbiA [H]

EC number: 2.5.1.- [C]

Molecular weight: Translated: 32875; Mature: 32875

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTLSHTPGSRLSQYLALTKPRVTQLAVFCAVIGMFLATPGMVPWKVLLGGTIGIGLLA
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHH
GSAFAINCLVEQKIDAMMRRTAWRPSARGEITTLQILAFSTVLGGLGAWTLYTFTNPLTM
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCHHHH
WLTIATFVGYAVIYTLLLKPMTPQNIVIGGASGAMPPALGWAAVTGAVPGDAWILVLIIF
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
VWTPPHFWVLALYRRKDYENAGLPMLPVTHGEQFTRLHILLYTVILFAVTMMPFISGMSG
HHCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVYLTSAVLLGALFLAYAWKIYRDYSDALARRAFRYSIVYLSLLFAALLVDHYARPVIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDTTLSHTPGSRLSQYLALTKPRVTQLAVFCAVIGMFLATPGMVPWKVLLGGTIGIGLLA
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHH
GSAFAINCLVEQKIDAMMRRTAWRPSARGEITTLQILAFSTVLGGLGAWTLYTFTNPLTM
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHCCHHHH
WLTIATFVGYAVIYTLLLKPMTPQNIVIGGASGAMPPALGWAAVTGAVPGDAWILVLIIF
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
VWTPPHFWVLALYRRKDYENAGLPMLPVTHGEQFTRLHILLYTVILFAVTMMPFISGMSG
HHCCCHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVYLTSAVLLGALFLAYAWKIYRDYSDALARRAFRYSIVYLSLLFAALLVDHYARPVIGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protoheme; farnesyl-PP [C]

Specific reaction: protoheme + farnesyl-PP = heme O + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA