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The map label for this gene is rhtB [H]

Identifier: 126453398

GI number: 126453398

Start: 3869861

End: 3870484

Strand: Direct

Name: rhtB [H]

Synonym: BURPS1106A_3965

Alternate gene names: 126453398

Gene position: 3869861-3870484 (Clockwise)

Preceding gene: 126453073

Following gene: 126453614

Centisome position: 97.03

GC content: 69.55

Gene sequence:

>624_bases
ATGTCCGTCGAAACCTGGCTCGCGTTCGCCGGCGCGAGCATCGTGCTTCTGATGATTCCCGGCCCGACGATCCTGCTCGT
GATCGGCGATTCGCTCGCGAACCGCCATCGGTCGTCGTGGAGCACCGTCGCGGGCGTCGCGGCGGGCGACACCACCGCGA
TGACGATCTCGCTCGCCGGCGCGGGCGCGCTGCTCGCGATGTCGGCGGCCGCGTTCACCGCGCTCAAGCTTGTCGGCGGC
GGCTATCTCGTCTATCTCGGCATCCGTTCGATTCTGCATGCGCGCCGCATCGGCGCGATGGACGTGCGCATCGAGCGCCG
TTCGGCGAAGCGGCGTTTCCTGTCCGCGTGGGCCGTCACCGCGCTCAATCCGAAGAGCATCGTGTTCTTCGTCGCGTTCG
TTCCGCAGTTCATCTCGCCGCACGAGACGTTCGCGCGGCAATGCGCGATCCTGCTGCCGACCTTCGTGTGCCTCGCGGCG
ATCAACGCGGCCTGCTATGCGCGCATCGCGCGTTTCGCCGCTGCGCGGCTGACGGGCGCCACCGCGCAGCGGCGCTTCGG
GTACGGCGGGGGCGCCGCGCTGATCGGCGCGGGCGCATTGACGCTCGGGATGTCGCGAAGCTGA

Upstream 100 bases:

>100_bases
GTCGACCATCCCGAGCCCGCTCGCCGATACGCTTGACAAGCCGTCCGCCGCCATGAATCACTGAGGGCTTTCGATTCGTG
CAACCGTAGCGGAGCCCCCC

Downstream 100 bases:

>100_bases
GGCCGGCACGCCACGCGCCGCGGGCCGCCCGGCTGCGCGCAACGATCCGCGCGGCGCCGCGCGGCATTCGTTGCCGATGC
GGGCGACGTGCACGCGACGT

Product: LysE family translocator protein

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSVETWLAFAGASIVLLMIPGPTILLVIGDSLANRHRSSWSTVAGVAAGDTTAMTISLAGAGALLAMSAAAFTALKLVGG
GYLVYLGIRSILHARRIGAMDVRIERRSAKRRFLSAWAVTALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLAA
INAACYARIARFAAARLTGATAQRRFGYGGGAALIGAGALTLGMSRS

Sequences:

>Translated_207_residues
MSVETWLAFAGASIVLLMIPGPTILLVIGDSLANRHRSSWSTVAGVAAGDTTAMTISLAGAGALLAMSAAAFTALKLVGG
GYLVYLGIRSILHARRIGAMDVRIERRSAKRRFLSAWAVTALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLAA
INAACYARIARFAAARLTGATAQRRFGYGGGAALIGAGALTLGMSRS
>Mature_206_residues
SVETWLAFAGASIVLLMIPGPTILLVIGDSLANRHRSSWSTVAGVAAGDTTAMTISLAGAGALLAMSAAAFTALKLVGGG
YLVYLGIRSILHARRIGAMDVRIERRSAKRRFLSAWAVTALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLAAI
NAACYARIARFAAARLTGATAQRRFGYGGGAALIGAGALTLGMSRS

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=202, Percent_Identity=31.6831683168317, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788099, Length=146, Percent_Identity=30.8219178082192, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21632; Mature: 21501

Theoretical pI: Translated: 11.88; Mature: 11.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVETWLAFAGASIVLLMIPGPTILLVIGDSLANRHRSSWSTVAGVAAGDTTAMTISLAG
CCHHHHHHHCCCEEEEEEECCCEEEEEECCHHHHHHCCCHHHHHEEECCCCEEEEEEECC
AGALLAMSAAAFTALKLVGGGYLVYLGIRSILHARRIGAMDVRIERRSAKRRFLSAWAVT
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHE
ALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLAAINAACYARIARFAAARLTGA
ECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
TAQRRFGYGGGAALIGAGALTLGMSRS
HHHHHCCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SVETWLAFAGASIVLLMIPGPTILLVIGDSLANRHRSSWSTVAGVAAGDTTAMTISLAG
CHHHHHHHCCCEEEEEEECCCEEEEEECCHHHHHHCCCHHHHHEEECCCCEEEEEEECC
AGALLAMSAAAFTALKLVGGGYLVYLGIRSILHARRIGAMDVRIERRSAKRRFLSAWAVT
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHEEEHHHHHHHHHHHHHHHE
ALNPKSIVFFVAFVPQFISPHETFARQCAILLPTFVCLAAINAACYARIARFAAARLTGA
ECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
TAQRRFGYGGGAALIGAGALTLGMSRS
HHHHHCCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]