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The map label for this gene is eno [H]

Identifier: 126453285

GI number: 126453285

Start: 2591235

End: 2592518

Strand: Reverse

Name: eno [H]

Synonym: BURPS1106A_2631

Alternate gene names: 126453285

Gene position: 2592518-2591235 (Counterclockwise)

Preceding gene: 126453670

Following gene: 126452195

Centisome position: 65.0

GC content: 64.88

Gene sequence:

>1284_bases
ATGAGTGCAATCGTAGACATCATCGGCCGCGAAATCCTTGATTCGCGCGGCAACCCCACCGTCGAGTGTGACGTGCTGCT
CGAATCGGGCACGATGGGCCGCGCAGCTGTGCCGTCGGGCGCGTCCACGGGCTCGCGCGAGGCGATCGAGCTGCGTGACG
GCGAAGCCGGCCGCTACGGCGGCAAGGGCGTGCTGAAGGCGGTCGAGCACATCAACACCGAGATCTCCGAAGCGATCATG
GGCCTCGATGCGTCCGAGCAGGCGTTCCTCGACAAGACGCTGCTCGAGCTCGACGGCACCGACAACAAGTCGCGCCTCGG
CGCGAACGCGATGCTCGCCGTGTCGATGGCGGTCGCGAAGGCCGCCGCCGAGGAAGCCGGCCTGCCGCTGTACCGCTACT
TCGGCGGCTCGGGCGCGATGCAATTGCCGGTGCCGATGATGAACATCGTCAACGGCGGCGCGCACGCGAACAACAGCCTC
GACATCCAGGAATTCATGATCGTTCCGGTCAGCCAGCCGACGTTCCGCGAAGCGCTGCGTTGCGGCGCGGAAGTGTTCCA
TGCGCTGAAGAAGATCCTCGGCGACCGCGGCATGAGCACGGCGGTCGGCGACGAAGGCGGCTTCGCGCCGAACTTCGGCA
GCAACGACGAATGCCTGTCGACGATCCTGCAGGCGATCGAGAAGGCCGGCTACCGTGCGGGCGAGGACGTGCTGCTCGCG
CTCGACTGCGCGGCATCCGAGTTCTACCACGACGGCAAGTACCAGCTCGCGGGCGAGGGCCTGCAACTGTCGTCGGCCGA
ATTCACCGATTACCTCGCGACGCTTGCCGACAAGTTCCCGATCGTGTCGATCGAGGACGGCATGCACGAAGGCGACTGGG
ACGGCTGGAAGCTGCTGACCGAGCGCCTCGGCAAGAAGGTGCAGCTCGTCGGCGACGACCTGTTCGTCACGAACACGCGC
ATCCTGAAGGAAGGCATCGAGAAGGGCATCGCGAACTCGATCCTCATCAAGATCAACCAGATCGGCACGTTGACCGAAAC
GTTCGCGGCGATCGAGATGGCCAAGCGCGCGCGCTACACGGCCGTGATCTCGCACCGCTCGGGCGAAACCGAGGATTCGA
CGATCGCCGACATCGCGGTGGGCCTGAACGCCGGCCAGATCAAGACGGGCTCGCTGTCGCGCAGCGATCGCATTTCGAAG
TACAACCAACTGCTGCGCATCGAAGAGGACCTGGGCGATATCGCGAGCTACCCGGGCAAATCGGCGTTCTACAATCTGCG
CTGA

Upstream 100 bases:

>100_bases
ACTCGGCGGCCGTAGCGCGCGTCAGTAGCAGGAGCGGCTGCCGCGCCGGATCGACCGCCGTCGCTGAAGACAGGATCCAT
CGTCATTTTCAGAGGAAACC

Downstream 100 bases:

>100_bases
CACGCGCTGACGCGTTACTATCGGTCTTGTTCACTGATGACGCCGCTTCGCGATCTCGTGAAGCGGCGCTTCTTATATCT
GCGGCTTATTCATGCGGCTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYGGKGVLKAVEHINTEISEAIM
GLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSL
DIQEFMIVPVSQPTFREALRCGAEVFHALKKILGDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHEGDWDGWKLLTERLGKKVQLVGDDLFVTNTR
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRARYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISK
YNQLLRIEEDLGDIASYPGKSAFYNLR

Sequences:

>Translated_427_residues
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYGGKGVLKAVEHINTEISEAIM
GLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSL
DIQEFMIVPVSQPTFREALRCGAEVFHALKKILGDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHEGDWDGWKLLTERLGKKVQLVGDDLFVTNTR
ILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRARYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISK
YNQLLRIEEDLGDIASYPGKSAFYNLR
>Mature_426_residues
SAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYGGKGVLKAVEHINTEISEAIMG
LDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAKAAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLD
IQEFMIVPVSQPTFREALRCGAEVFHALKKILGDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLAL
DCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHEGDWDGWKLLTERLGKKVQLVGDDLFVTNTRI
LKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRARYTAVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKY
NQLLRIEEDLGDIASYPGKSAFYNLR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=437, Percent_Identity=51.7162471395881, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=49.1879350348028, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897477, Length=437, Percent_Identity=48.9702517162471, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=437, Percent_Identity=48.9702517162471, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=428, Percent_Identity=44.392523364486, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=25.8928571428571, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=25.8928571428571, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=25.8928571428571, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=65.8823529411765, Blast_Score=555, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=49.4279176201373, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=49.4279176201373, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=44.1624365482233, Blast_Score=169, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=46.0829493087558, Blast_Score=370, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=46.9907407407407, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=46.9907407407407, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=46.9907407407407, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=47.0046082949309, Blast_Score=355, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=49.5327102803738, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=49.5327102803738, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=49.5327102803738, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=49.5327102803738, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=49.5327102803738, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=49.5327102803738, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45683; Mature: 45552

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYG
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVEHINTEISEAIMGLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLDIQEFMIVPVSQPTFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFHALKKILGDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHEGDWDGWKLLT
EEHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
ERLGKKVQLVGDDLFVTNTRILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRARYT
HHCCCEEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCEECCC
>Mature Secondary Structure 
SAIVDIIGREILDSRGNPTVECDVLLESGTMGRAAVPSGASTGSREAIELRDGEAGRYG
CHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVEHINTEISEAIMGLDASEQAFLDKTLLELDGTDNKSRLGANAMLAVSMAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAAEEAGLPLYRYFGGSGAMQLPVPMMNIVNGGAHANNSLDIQEFMIVPVSQPTFREALR
HHHHHCCCCEEEEECCCCCEECCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
CGAEVFHALKKILGDRGMSTAVGDEGGFAPNFGSNDECLSTILQAIEKAGYRAGEDVLLA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
LDCAASEFYHDGKYQLAGEGLQLSSAEFTDYLATLADKFPIVSIEDGMHEGDWDGWKLLT
EEHHHHHHHCCCCEEEECCCCEECCHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHH
ERLGKKVQLVGDDLFVTNTRILKEGIEKGIANSILIKINQIGTLTETFAAIEMAKRARYT
HHCCCEEEEECCCEEEEHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHE
AVISHRSGETEDSTIADIAVGLNAGQIKTGSLSRSDRISKYNQLLRIEEDLGDIASYPGK
EEEECCCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
SAFYNLR
CCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA