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The map label for this gene is ddl [H]

Identifier: 126453261

GI number: 126453261

Start: 3451447

End: 3452385

Strand: Reverse

Name: ddl [H]

Synonym: BURPS1106A_3548

Alternate gene names: 126453261

Gene position: 3452385-3451447 (Counterclockwise)

Preceding gene: 126454974

Following gene: 126452609

Centisome position: 86.56

GC content: 66.99

Gene sequence:

>939_bases
ATGAGCGGGATCGATCCGAAACGTTTCGGCAAGGTGGCGGTGCTGCTCGGCGGGGATTCCGCCGAGCGCGAGGTATCGCT
GAACTCTGGCCGGCTGGTGCTGCAGGGCCTGCGCGACGCGGGCATCGACGCGCATCCGTTCGATCCGGCGCAGCGGCCGC
TCGCGGCGCTCAAGGACGAAGGCTTCGTGCGCGCGTTCAACGCGCTGCACGGCGGCTACGGCGAGAACGGCCAGATCCAG
GGCGCGCTCGATTTCTACGGCATCCGCTACACGGGCAGCGGCGTGCTCGGCTCGGCGCTCGGGCTCGACAAGTTCCGCAC
GAAGCTCGTCTGGCAGCAGACGGGCATTCCGACGCCGCCGTTCGAGACGGTGATGCGCGGCGACGATTATGCGGCGCGCG
CGCAGGACATCGTCGCGAAGCTCGGCGTGCCGCTTTTCGTGAAGCCGGCGAGCGAGGGCTCGAGCGTCGCGGTCGAGAAG
GTGAAAAGCGCCGATGCGTTGCCCGCCGCACTGGAAGAAGCGGCGAAGCACGACAAGATCGTGATCGTCGAGAAGAGCAT
CGAAGGCGGCGGCGAATACACCGCGTGCATCGCCGCGGACCTCGATCTGCCGCTCATCAGGATCGTGCCCGCGGGCGAGT
TCTACGACTATCACGCGAAGTACATCGCGAACGACACGCAATACCTGATTCCGTGCGGCCTCGACGCGGCAAAGGAAGCC
GAGTTCAAGCGCATCGCGCGCCGCGCGTTCGACGTGCTCGGCTGCACGGACTGGGGCCGCGCGGATTTCATGCTCGACGC
CGCGGGCAACCCGTATTTCCTCGAAGTGAACACCGCGCCGGGGATGACCGATCACTCGCTGCCGCCGAAGGCGGCGCGCG
CGGTGGGCATCGGCTATTCGGAGCTGGTGGTGAAAGTGCTGTCGCTCACGCTCGATTGA

Upstream 100 bases:

>100_bases
AAGCTCGCGCGCGACGGCGACGTGGTGATCACGATGGGCGCGGGCTCGATCGGCGGCGTGCCGGGCAAGCTCGCGCAGGA
CACGCAACAGAAGGGATGAC

Downstream 100 bases:

>100_bases
TGCAGGACCGGACTTGAACCATGTGGAATAACGTTCGCCAACTCAACCTTGCCGCCAGCGCGTTGTACGCGCTGTTGCTG
CTCGTGTTGGCGGCGGCCGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSGIDPKRFGKVAVLLGGDSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAQRPLAALKDEGFVRAFNALHGGYGENGQIQ
GALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGIPTPPFETVMRGDDYAARAQDIVAKLGVPLFVKPASEGSSVAVEK
VKSADALPAALEEAAKHDKIVIVEKSIEGGGEYTACIAADLDLPLIRIVPAGEFYDYHAKYIANDTQYLIPCGLDAAKEA
EFKRIARRAFDVLGCTDWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGIGYSELVVKVLSLTLD

Sequences:

>Translated_312_residues
MSGIDPKRFGKVAVLLGGDSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAQRPLAALKDEGFVRAFNALHGGYGENGQIQ
GALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGIPTPPFETVMRGDDYAARAQDIVAKLGVPLFVKPASEGSSVAVEK
VKSADALPAALEEAAKHDKIVIVEKSIEGGGEYTACIAADLDLPLIRIVPAGEFYDYHAKYIANDTQYLIPCGLDAAKEA
EFKRIARRAFDVLGCTDWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGIGYSELVVKVLSLTLD
>Mature_311_residues
SGIDPKRFGKVAVLLGGDSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAQRPLAALKDEGFVRAFNALHGGYGENGQIQG
ALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGIPTPPFETVMRGDDYAARAQDIVAKLGVPLFVKPASEGSSVAVEKV
KSADALPAALEEAAKHDKIVIVEKSIEGGGEYTACIAADLDLPLIRIVPAGEFYDYHAKYIANDTQYLIPCGLDAAKEAE
FKRIARRAFDVLGCTDWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGIGYSELVVKVLSLTLD

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=50.3311258278146, Blast_Score=302, Evalue=1e-83,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=32.2857142857143, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33341; Mature: 33210

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIDPKRFGKVAVLLGGDSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAQRPLAALKDE
CCCCCCCCCCCEEEEECCCCCCCEEECCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHCCC
GFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHCCCCCCCC
FETVMRGDDYAARAQDIVAKLGVPLFVKPASEGSSVAVEKVKSADALPAALEEAAKHDKI
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCEE
VIVEKSIEGGGEYTACIAADLDLPLIRIVPAGEFYDYHAKYIANDTQYLIPCGLDAAKEA
EEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHEEECCCCEEEECCCCHHHHH
EFKRIARRAFDVLGCTDWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGIGYS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVKVLSLTLD
HHHHHHHHHHCC
>Mature Secondary Structure 
SGIDPKRFGKVAVLLGGDSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAQRPLAALKDE
CCCCCCCCCCEEEEECCCCCCCEEECCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHCCC
GFVRAFNALHGGYGENGQIQGALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHCCCCCCCC
FETVMRGDDYAARAQDIVAKLGVPLFVKPASEGSSVAVEKVKSADALPAALEEAAKHDKI
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHCCCEE
VIVEKSIEGGGEYTACIAADLDLPLIRIVPAGEFYDYHAKYIANDTQYLIPCGLDAAKEA
EEEEECCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHEEECCCCEEEECCCCHHHHH
EFKRIARRAFDVLGCTDWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGIGYS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVKVLSLTLD
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA