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The map label for this gene is rrmJ [H]

Identifier: 126453006

GI number: 126453006

Start: 1467208

End: 1467870

Strand: Direct

Name: rrmJ [H]

Synonym: BURPS1106A_1511

Alternate gene names: 126453006

Gene position: 1467208-1467870 (Clockwise)

Preceding gene: 126455156

Following gene: 126452144

Centisome position: 36.79

GC content: 62.29

Gene sequence:

>663_bases
ATGGCAAAGAACCGCTTTAACCAATCCTGGTTGCACGACCACATCAACGACCCGTACGTCAAAATGGCGCAGCGGGAAGG
GTACCGCGCGCGCGCCGCGTACAAGCTGAAGGAAATCGACGAGCAGGACAAGCTGATCCGCCCGGGCCAGGTGATCGTCG
ATCTGGGCGCGGCGCCGGGCAGCTGGAGCCAGTACGCGCGCAACAAGCTCGCGCAGGGCAAGCGGCGCGACGCGGTGCGC
GAGGGCGGCATCGACGGCACGATCATCGCGCTCGACATGCTGCCGATGGAGCCCGTCGCCGACGTCCATTTCATCCAGGG
CGACTTCCGCGAGGAGAGCGTGCTGCATCAATTGGAGGAAGTCCTCGCCGGGCGCGCGGTCGACCTTGTAATTTCCGACA
TGGCCCCCAACCTGTCCGGCGTGGCGGTCGCGGATGCGGCGCGGATCGAGCATGTCTGCGATCTGGCGCTCGAATTCGCG
CAAAACCATCTGAAGCCGGATGGCGCCCTTTTGGTGAAATGCTTTCACGGCAGCGGCTACAGCCAGATCGTCGAGAAATT
CAAGCACCAATTTAAAACGGTGGCGCCGCGCAAGCCAAAAGCTTCCCGCGACAAATCGTCCGAAACGTTTATTTTGGGTC
GGCATCTGAAGCAGCCGCGCTGA

Upstream 100 bases:

>100_bases
CGGCTTGGGCCCTTCGGGGCCGAAAGCCGCGCCAATAAGTCCCGTATTATCCGCCAAAAGCGCGGCTTCACGAAGCAATC
GGCAGCAATCTCTCCATCGC

Downstream 100 bases:

>100_bases
ACGTGGCATCGCGCGGGAGGATGCGCCGGAGCCCTTGCCAGCCGTGCCGCGCGCCTATCGGCGCGGTAGCGAATCCTTAT
GGCGAGGGGCTGCGGACTGG

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLAQGKRRDAVR
EGGIDGTIIALDMLPMEPVADVHFIQGDFREESVLHQLEEVLAGRAVDLVISDMAPNLSGVAVADAARIEHVCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKQPR

Sequences:

>Translated_220_residues
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLAQGKRRDAVR
EGGIDGTIIALDMLPMEPVADVHFIQGDFREESVLHQLEEVLAGRAVDLVISDMAPNLSGVAVADAARIEHVCDLALEFA
QNHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKQPR
>Mature_219_residues
AKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPGSWSQYARNKLAQGKRRDAVRE
GGIDGTIIALDMLPMEPVADVHFIQGDFREESVLHQLEEVLAGRAVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQ
NHLKPDGALLVKCFHGSGYSQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKQPR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=219, Percent_Identity=35.6164383561644, Blast_Score=138, Evalue=3e-33,
Organism=Homo sapiens, GI29029591, Length=203, Percent_Identity=32.0197044334975, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI29029589, Length=203, Percent_Identity=32.0197044334975, Blast_Score=107, Evalue=7e-24,
Organism=Homo sapiens, GI7110661, Length=203, Percent_Identity=32.0197044334975, Blast_Score=107, Evalue=8e-24,
Organism=Homo sapiens, GI194097365, Length=203, Percent_Identity=29.064039408867, Blast_Score=107, Evalue=9e-24,
Organism=Escherichia coli, GI1789569, Length=203, Percent_Identity=43.3497536945813, Blast_Score=184, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17553474, Length=226, Percent_Identity=32.3008849557522, Blast_Score=113, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI17554650, Length=203, Percent_Identity=31.5270935960591, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71987550, Length=203, Percent_Identity=30.0492610837438, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=203, Percent_Identity=30.0492610837438, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17553860, Length=203, Percent_Identity=30.0492610837438, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319535, Length=205, Percent_Identity=29.7560975609756, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321302, Length=133, Percent_Identity=34.5864661654135, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21356387, Length=218, Percent_Identity=34.4036697247706, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24648639, Length=206, Percent_Identity=31.5533980582524, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.3529411764706, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI18859957, Length=198, Percent_Identity=28.7878787878788, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 24661; Mature: 24529

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKRRDAVREGGIDGTIIALDMLPMEPVADVHFIQGDFREESVLHQLEE
CHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHH
VLAGRAVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKQPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
AKNRFNQSWLHDHINDPYVKMAQREGYRARAAYKLKEIDEQDKLIRPGQVIVDLGAAPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCC
SWSQYARNKLAQGKRRDAVREGGIDGTIIALDMLPMEPVADVHFIQGDFREESVLHQLEE
CHHHHHHHHHHCCHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHH
VLAGRAVDLVISDMAPNLSGVAVADAARIEHVCDLALEFAQNHLKPDGALLVKCFHGSGY
HHCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCH
SQIVEKFKHQFKTVAPRKPKASRDKSSETFILGRHLKQPR
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA