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The map label for this gene is aes [H]

Identifier: 126452981

GI number: 126452981

Start: 2300878

End: 2301843

Strand: Reverse

Name: aes [H]

Synonym: BURPS1106A_2325

Alternate gene names: 126452981

Gene position: 2301843-2300878 (Counterclockwise)

Preceding gene: 126454508

Following gene: 126452096

Centisome position: 57.71

GC content: 69.98

Gene sequence:

>966_bases
TTGACGATGCCGCTGAATCCGAAGATCGCGCAGGTGCTCGACATGATCGAGCGCGCCAAGCGTCCCGATTATCATGAACA
GACGCCCGCGCAGGCGCGCGCCGCGTACGAGAAGAGCGCGCCGATCCTCGACGTCGCGCCCGCGCCGATGTTTTCGGTCG
AAGACCTGCGCGTGCCGTCGCGCGACGGCGGCGCGTTCGGCGCGCGGCTCTATCTGCCCGTCGAGCCGAGTCTCGCCGAG
CCGCTGCCCGTGCTCGTCTACTACCACGGCGGCGGCTTCACGGTCGGCAGCGTGAACACGCACGACGCGCTGTGCCGGAT
GTTCGCGCGCGATGCGCAGTGCGCGGTGCTGTCGGTGGATTACCGGCTCGCGCCGGAGCACAAGTTCCCGACCGCCGTCG
ACGACGCGGAGGACGCGCTCGTGTGGCTGCATGCGCACGCGTCGCGCTTCGGAATCGATTCGGCGCGGCTCGCGGTGGGC
GGCGACAGCGCGGGCGGCACGCTTGCGACGGTGTGCGCGGTGCTCGCGCGCGATCGCGGGATCGCGCTCGCGCTGCAACT
GCTGATCTATCCGGGCACGGTGGGGCATCAGCAGACCGAATCGCACGCGCGGCTCGCGAAAGGCTATCTGTTGTCCGCGG
ACACGATCCAGTGGTTTTTCGGGCATTACGTGCGCGATGCGTCGGATCGCGACGACTGGCGCTTCGCGCCGCTCGACGGC
ACGCGCGGCGCGCCGTCGTTCGAGCGCGTCGCGCCCGCGTGGATCGCGACGGCGCAGTACGATCCGCTATCCGACGAAGG
CGAAGCGTATGCGGACAAGCTGCGCGCGGCGGGCAACCGCGTGACGCTCGTCGCGTACGCGGGGATGATTCACGAGTTCT
TCAAGATGGGCGGCTACGTGCCGGAGGTGCGGCTCGCGCATGCGGACGCGGTGGGCGCGCTGCGCGCGGCGTTCGACGGC
GATTGA

Upstream 100 bases:

>100_bases
ACGCGCCGTGGCATCATACGGCGCTTGTCCCGGGCCTTTCACCGGCGCGCCCGCGCCGTTCGCCCGCCGTTTGCGTTGCT
GTTTGCCCCGCCGTTTGCCC

Downstream 100 bases:

>100_bases
GCGTGCGCGCGGGCGGGTGACGGCGCACGGTGCGGCGCGGCATGTGCCGGACAGGGCGTGCGGCGACGGCCACGACGAAG
GCGGCGCGGTCCGCCGCGGC

Product: carboxylesterase Est2

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRDGGAFGARLYLPVEPSLAE
PLPVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVG
GDSAGGTLATVCAVLARDRGIALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDG
TRGAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPEVRLAHADAVGALRAAFDG
D

Sequences:

>Translated_321_residues
MTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRDGGAFGARLYLPVEPSLAE
PLPVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVG
GDSAGGTLATVCAVLARDRGIALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDG
TRGAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPEVRLAHADAVGALRAAFDG
D
>Mature_320_residues
TMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPSRDGGAFGARLYLPVEPSLAEP
LPVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVDYRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGG
DSAGGTLATVCAVLARDRGIALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDGT
RGAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYVPEVRLAHADAVGALRAAFDGD

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=273, Percent_Identity=27.8388278388278, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI157041239, Length=110, Percent_Identity=40, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI61966717, Length=217, Percent_Identity=27.1889400921659, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI68299767, Length=153, Percent_Identity=35.9477124183007, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI68051721, Length=273, Percent_Identity=26.007326007326, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI21328446, Length=112, Percent_Identity=32.1428571428571, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI226423947, Length=281, Percent_Identity=24.9110320284698, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1786682, Length=255, Percent_Identity=31.3725490196078, Blast_Score=116, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI72001146, Length=343, Percent_Identity=25.9475218658892, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996133, Length=291, Percent_Identity=27.1477663230241, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=29.4339622641509, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17540028, Length=148, Percent_Identity=36.4864864864865, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24656084, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24656076, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20130169, Length=106, Percent_Identity=35.8490566037736, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34767; Mature: 34635

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPS
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHCCCCC
RDGGAFGARLYLPVEPSLAEPLPVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVD
CCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEEE
YRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGGDSAGGTLATVCAVLARDRG
EEECCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCC
IALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDG
HHEEEEHHHCCCCCCCCCHHHHHHHHHCEEECHHHHHHHHHHHHHCCCCCCCCEEECCCC
TRGAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYV
CCCCCCHHHHCHHHEEECCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCC
PEVRLAHADAVGALRAAFDGD
CCEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TMPLNPKIAQVLDMIERAKRPDYHEQTPAQARAAYEKSAPILDVAPAPMFSVEDLRVPS
CCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCHHHCCCCC
RDGGAFGARLYLPVEPSLAEPLPVLVYYHGGGFTVGSVNTHDALCRMFARDAQCAVLSVD
CCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHCCCCEEEEEEE
YRLAPEHKFPTAVDDAEDALVWLHAHASRFGIDSARLAVGGDSAGGTLATVCAVLARDRG
EEECCCCCCCCCCCCCCCEEEEEEECHHHCCCCCCEEEECCCCCCCHHHHHHHHHHCCCC
IALALQLLIYPGTVGHQQTESHARLAKGYLLSADTIQWFFGHYVRDASDRDDWRFAPLDG
HHEEEEHHHCCCCCCCCCHHHHHHHHHCEEECHHHHHHHHHHHHHCCCCCCCCEEECCCC
TRGAPSFERVAPAWIATAQYDPLSDEGEAYADKLRAAGNRVTLVAYAGMIHEFFKMGGYV
CCCCCCHHHHCHHHEEECCCCCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCC
PEVRLAHADAVGALRAAFDGD
CCEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA