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The map label for this gene is cysD [H]

Identifier: 126452905

GI number: 126452905

Start: 2517783

End: 2518748

Strand: Reverse

Name: cysD [H]

Synonym: BURPS1106A_2563

Alternate gene names: 126452905

Gene position: 2518748-2517783 (Counterclockwise)

Preceding gene: 126453005

Following gene: 126452895

Centisome position: 63.15

GC content: 68.63

Gene sequence:

>966_bases
ATGAACGCAACGCTCGAACCATCTTCGCCGGGCGGGGCCGCCGGCGCGTCGACGAGCCGGATGGGCCATCTCGACTGGCT
CGAGGCCGAGTCGATCCATATCCTGCGCGAGCTCGTCGCGGAATGCAGCAAGCCGGCGCTGCTGTTCTCGGGCGGCAAGG
ATTCGGTGGTCGTGCTGCATCTCGCGCTCAAGGCGTTCGGGCTCGGCGCGAACCGCAGGACGACGCTGCCGTTTCCGCTC
GTGCACATCGACACGGGCCACAACTACGACGAGGTGATCGATTTCCGCGACCGCCGCGCAAAGCAGATCGGCGCGGAGCT
GGTGGTCGGCCACGTCGAGGATTCGATCGCGCGCGGCACGGTGGTGCTGCGCCGCGAGACGGATTCGCGCAACGCCGCGC
AGGCGGTCACGCTGCTCGAGACGATCGAGCGGCACGGCTACACGGCGATGATCGGCGGGGCGCGGCGCGACGAGGAGAAG
GCGCGGGCGAAGGAGCGGATTTTCTCGTTTCGCGACGAATTCGGCCAGTGGGACCCGAAGGCGCAGCGCCCGGAGCTGTG
GAGCCTGTACAACGCGCGGCTGCACCGGGGCGAACACCTGCGGGTGTTCCCGATCTCGAACTGGACGGAGCTCGACGTGT
GGCAGTACATCGCGCGCGAGAAGCTGGAGCTGCCGTCGATCTACTACGCGCACCGCCGGGAGATCGTGCGGCGCAACGGG
CTGCTCGTGCCGGTGACGCCGCTCACGCCGATGCGCGAGGGCGAGACGAGCGAGCAGGCGCTGGTGCGGTTCCGCACGGT
GGGGGACATTTCGTGCACGTGCCCGGTCGAGAGCGACGCGGACGACGTGGAGAAGATCATCGCGGAGACGGCGGTGACGG
AGATCACGGAGCGCGGGGCGACGCGGATGGACGACCAGGCGTCGGAGGCCGCGATGGAGCAGCGCAAGAAGCAGGGCTAT
TTCTGA

Upstream 100 bases:

>100_bases
TCGCCGCGGGCCCCATCGGCGGGCGGAACCGGCGGGCGGAACCGGCGCCGCCGCATCGCGGCCGTCGCGCCGCAGGGGTT
CGGAACGGGGGAGCGGAACC

Downstream 100 bases:

>100_bases
CGCACGCGAGGACACATCCATCATGAGCATCATCGAGAACAACGAAGACCTCGGCGTACTGCGGTTCATCACGGCGGGCA
GCGTCGACGACGGCAAGCGC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNATLEPSSPGGAAGASTSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPL
VHIDTGHNYDEVIDFRDRRAKQIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEK
ARAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNG
LLVPVTPLTPMREGETSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGY
F

Sequences:

>Translated_321_residues
MNATLEPSSPGGAAGASTSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPL
VHIDTGHNYDEVIDFRDRRAKQIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEK
ARAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNG
LLVPVTPLTPMREGETSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGY
F
>Mature_321_residues
MNATLEPSSPGGAAGASTSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRRTTLPFPL
VHIDTGHNYDEVIDFRDRRAKQIGAELVVGHVEDSIARGTVVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEK
ARAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNG
LLVPVTPLTPMREGETSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGY
F

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=304, Percent_Identity=46.3815789473684, Blast_Score=269, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36120; Mature: 36120

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATLEPSSPGGAAGASTSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLH
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHH
LALKAFGLGANRRTTLPFPLVHIDTGHNYDEVIDFRDRRAKQIGAELVVGHVEDSIARGT
HHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCE
VVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPK
EEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNG
CCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
LLVPVTPLTPMREGETSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGA
CEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
TRMDDQASEAAMEQRKKQGYF
CCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNATLEPSSPGGAAGASTSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLH
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHH
LALKAFGLGANRRTTLPFPLVHIDTGHNYDEVIDFRDRRAKQIGAELVVGHVEDSIARGT
HHHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHCCE
VVLRRETDSRNAAQAVTLLETIERHGYTAMIGGARRDEEKARAKERIFSFRDEFGQWDPK
EEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AQRPELWSLYNARLHRGEHLRVFPISNWTELDVWQYIAREKLELPSIYYAHRREIVRRNG
CCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
LLVPVTPLTPMREGETSEQALVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGA
CEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCC
TRMDDQASEAAMEQRKKQGYF
CCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]