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The map label for this gene is pyk [H]

Identifier: 126452857

GI number: 126452857

Start: 820021

End: 821457

Strand: Direct

Name: pyk [H]

Synonym: BURPS1106A_0840

Alternate gene names: 126452857

Gene position: 820021-821457 (Clockwise)

Preceding gene: 126454228

Following gene: 126451721

Centisome position: 20.56

GC content: 68.06

Gene sequence:

>1437_bases
ATGCACCGCGCCACCAAGATAGTCGCCACGATCGGTCCGGCATCCAGCTCGCCGGAAGTCCTGTCGCAGATGATGCACGC
GGGTCTCGACGTCGTGCGGCTCAATTTCTCGCACGGCTCCGCCGACGATCACCGCGAGCGCGCCGAGATGGTCCGCGAGG
CCGCGCGGCGGGTCGGCCGGGAAATCGCGATCATGGCGGACCTGCAGGGGCCGAAGATCCGGGTCGGCAAGTTCGAGGCG
GGCAAGACGACGCTCACGCCCGGCCAGCCGTTCATCCTCGACGCGACGTGCGAGCTCGGCAACGACGAGCGGGTCGGCCT
CGACTACAAGGATCTGCCGCGCGACCTGAAGCCGGGCGACGTGCTGCTGCTCAACGACGGCCTGATCGTGCTGAAGGTCG
ATCGCGTGCTCGGCGACGAGATCCACACGACCGTCAAGGTGGGCGGCGATCTGTCGAACAACAAGGGGATCAACCGGCAG
GGCGGCGGGCTGTCGGCGCCCGCGCTGACCGCGAAGGACATGGAGGACATCCGCACCGCGATGTCGCTCGGCGCGGATCT
CGTCGCGGTGTCGTTCCCGAAGAACGCGACCGACATGGAGATGGCGCGCCAGCTCGCGAACATCGCGGGCGCGCCTTACG
GCATCAAGCCGAAGATGATCGCGAAGATCGAGCGCGCGGAGGCGATTCCGGCGCTGCAGGAGATTCTCGATGCGTCGGAC
GGCATCATGGTCGCGCGCGGGGACCTCGCCGTCGAGGTCGGCAACGCGGCCGTGCCCGCGCTGCAGAAGCGGATGATCCG
GATGGCGCGCGAGTCGAACAAGCTCGTGATCACCGCGACGCAGATGATGGAATCGATGATCCACGCGCCGGTGCCGACGC
GCGCGGAAGTGTCGGACGTCGCGAACGCGGTGCTCGACGGCACCGACGCGGTGATGCTGTCGGCCGAGACGGCGACGGGC
AAGTACCCGGCCGTCACGATCGAGGCGATGGCGGCCGTCTGCGTCGAGGCGGAGAAATCCGAGCACGTCGAGCTCGACAA
GGATTTTCTCGACCGCACGTTCACGCGGATCGACCAGTCGATCGCGATGGGCGCGCTTTTCACCGCGTACCACCTGGGCG
CGAAGGCGATCGTCGCGCTGACCGAATCGGGCGCGACCGCGCTGTGGATGTCGCGCCACTACACGCACGTGCCGATCTTC
GCGCTCACGCCGCGGGTCGGCAGCGAGCGTGCGATGGCGCTGTTTCGCAACGTGACGCCGCTGCACGTGGACTTCAACAG
CGACCGCGATTCCGCGCTGCAGCAGGCGCTCGAACTGATCGTGCGGCGCGGCTATGTCGCGCACGGCGACATGGTCGTGC
TGACGGTGGGCGAGCCGATGGGGCAGGCGGGCGGCACGAACACGCTGAAGATCGTGCGCGTGGGCGAGCACTACTGA

Upstream 100 bases:

>100_bases
CGCACCGGCGCGCGTTCCGAAGCGGCGGCGGCTGGGCCGCGCGGCTTCGGCTCTCGCGGCGTTACATCCAGCGCTTTCAC
TCAGTCAGACGAGGAGATTC

Downstream 100 bases:

>100_bases
AGCAACCGAACGGGCGTTCGGCGCCCGGGCGCGCCGGGCACGCCGGGCACGCCCGTTGCGCGCGCAAAGAGCCGCGGGGT
CGGACGCCTCGCGGCTTTTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MHRATKIVATIGPASSSPEVLSQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIRVGKFEA
GKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVLGDEIHTTVKVGGDLSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQEILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETATG
KYPAVTIEAMAAVCVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIF
ALTPRVGSERAMALFRNVTPLHVDFNSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY

Sequences:

>Translated_478_residues
MHRATKIVATIGPASSSPEVLSQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIRVGKFEA
GKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVLGDEIHTTVKVGGDLSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQEILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETATG
KYPAVTIEAMAAVCVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIF
ALTPRVGSERAMALFRNVTPLHVDFNSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
>Mature_478_residues
MHRATKIVATIGPASSSPEVLSQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGREIAIMADLQGPKIRVGKFEA
GKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGDVLLLNDGLIVLKVDRVLGDEIHTTVKVGGDLSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQEILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETATG
KYPAVTIEAMAAVCVEAEKSEHVELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIF
ALTPRVGSERAMALFRNVTPLHVDFNSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=36.7139959432049, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=36.7139959432049, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=37.5, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=37.2983870967742, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=36.3083164300203, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.426116838488, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.426116838488, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.4728033472803, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.4728033472803, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.1926605504587, Blast_Score=105, Evalue=8e-23,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=52.3012552301255, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=39.0658174097665, Blast_Score=306, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.4754098360656, Blast_Score=283, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=38.3983572895277, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=38.3983572895277, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=38.3983572895277, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=37.782340862423, Blast_Score=265, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=36.3821138211382, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6319279, Length=490, Percent_Identity=34.6938775510204, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=38.9452332657201, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=38.9452332657201, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24648966, Length=464, Percent_Identity=36.6379310344828, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=31.9018404907975, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=36.7647058823529, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51371; Mature: 51371

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRATKIVATIGPASSSPEVLSQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
EIAIMADLQGPKIRVGKFEAGKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGD
EEEEEEECCCCEEEEEEECCCCEECCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
VLLLNDGLIVLKVDRVLGDEIHTTVKVGGDLSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQEILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAETATGKYPAVTIEAMAAVCVEAEKSEHVELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIFALTPRVGSERAMALFRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCE
LHVDFNSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
EEEECCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MHRATKIVATIGPASSSPEVLSQMMHAGLDVVRLNFSHGSADDHRERAEMVREAARRVGR
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
EIAIMADLQGPKIRVGKFEAGKTTLTPGQPFILDATCELGNDERVGLDYKDLPRDLKPGD
EEEEEEECCCCEEEEEEECCCCEECCCCCCEEEEEEECCCCCCCCCCCHHHCCCCCCCCC
VLLLNDGLIVLKVDRVLGDEIHTTVKVGGDLSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQEILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAETATGKYPAVTIEAMAAVCVEAEKSEHVELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIFALTPRVGSERAMALFRNVTP
HHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCE
LHVDFNSDRDSALQQALELIVRRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
EEEECCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]