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The map label for this gene is emrB [H]

Identifier: 126452845

GI number: 126452845

Start: 1594446

End: 1596065

Strand: Reverse

Name: emrB [H]

Synonym: BURPS1106A_1642

Alternate gene names: 126452845

Gene position: 1596065-1594446 (Counterclockwise)

Preceding gene: 126452822

Following gene: 126451768

Centisome position: 40.02

GC content: 66.54

Gene sequence:

>1620_bases
ATGAACGGATCGACGCCCACCCCGCCCGCCGCACCGCGCCGCGACCGCAACGGCAACGGCGACGGCACGTGGCGTCCCGC
CGCGAACCCGTGGCTGATCGCGGTCGTCGTCACGCTCGCCGCGTTCATGGAGGTGCTCGACACGACGATCGTCAACGTCG
CGCTGCCGCACATCGCCGGCACGATGTCGGCGAGCTACGACGAAGCGACCTGGACGCTCACGTCGTATCTTGTCGCCAAC
GGCATCGTGCTGCCGATCTCCGGCTTTCTCGGCCGCCTGCTCGGCCGCAAGCGCTACTTCGTGCTGTGCATCGTCGCGTT
CACGATCTGCTCGTTCCTGTGCGGAATCGCAACCGATCTCGGCCAACTGATCGTGTTCCGCGTGCTGCAGGGGCTGTTCG
GCGGCGGCCTGCAGCCGAACCAGCAATCGATCATCCTCGATACGTTCCCGCCCGAGCAGCGCAACCGCGCGTTCTCGATC
TCGGCCGTGGCGATCGTCGTCGCGCCGGTGCTCGGCCCGACGCTCGGCGGCTGGATCACCGACAACTTCTCGTGGCGCTG
GGTGTTCCTGCTCAACGTGCCGATCGGCGTGCTGACTTCGCTCGCGGTGATCCAGCTCGTCGAGGATCCGCCGTGGAAAC
GCGGCCGCGCGCGCGGCCTGTCGATCGACTACATCGGCATCACGCTGATCGCGATCGGCCTCGGCTGTCTGCAGGTGATG
CTCGATCGCGGCGAGGACGAGGACTGGTTCGCGTCGACGTTCATCCGCACGTTCGCGGTGCTGACCGTCGCGGGGCTCGT
CGGCGCGACGTTCTGGCTGCTCTACGCGAAAAAGCCCGTCGTCGATCTGTCGTGCCTGAAGGATCGCAATTTCGCGCTCG
GCTGCGTGACGATCGCGACGTTCGCGGTCGTGCTGTACGGCAGCGCCGTGCTCGTGCCGCAACTCGCGCAGCAGCGGCTC
GGCTACACGGCGATGCTCGCGGGTCTCGTGCTGTCGCCAGGCGCGCTCCTCATCACGCTCGAGATTCCGATCGTCAGCAA
GCTGATGCCGTACGTGCAGACGCGCTTTCTCGTATGCTTCGGCTTCCTGCTGCTCGCCGCGTCGCTCGTGTACTCGCACA
CGCTCGTGCCCGACATCGACTATCCGAACCTCCTGAAGATACGCAGCGCGCAATCGCTCGCGATCGGCTTCCTGTTCGTG
CCGATCACGACGCTCGCGTATCTGACCGTGCCGCGCCGGCTGAACGACGATGCGTCCGCGCTTTTCACGATGTTCCGCAA
TGTCGCGGGATCGATCGGCATTTCGCTGTCGACCGCGCTGATTCGCGAGCGCGCGCAGGCGCGCATGGCGCATCTGTCGA
CGCACCTGTCGCCGCTGTCGCAGAACTTCGACGATACGCTGCGGCAGAACGCGCAGACGATCGCATCGCTCTCGGGGATG
CCGCCGCCCGCCGCGCTGCAAACGGCGAGCGGGCAGTTGTACGAGACCTTCGTGTCGCAGGCGACGATCCTCGCGTACCT
CGACGTGTTCGCGATGCTCGCGATCTTCTGCTTCGTGTGCACGCCCCTCACGCTGCTTTTCTCGCCCGTGAAGGCGGCGG
GCGGCCCGGGAGGACACTGA

Upstream 100 bases:

>100_bases
GTATCCATCCCGTTCGCCGCATTCGCCGCGCCGCCGCGCCTGAGCGCGCACGCTTGGCCGCCGTCGGCGGCGCCGCATCG
TCTCTTCTCGCCCCGTGCCG

Downstream 100 bases:

>100_bases
TATGCGGCGCGCACTCACGCGATGGCGGCTGTCGTTCGCGCTCGGCGCGCTCGCCGCGCTCGCGAGCGCGTGCACGGTCG
GGCCGGACTTTCATCCTCCG

Product: drug:H+ antiporter-2 (DHA2) family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MNGSTPTPPAAPRRDRNGNGDGTWRPAANPWLIAVVVTLAAFMEVLDTTIVNVALPHIAGTMSASYDEATWTLTSYLVAN
GIVLPISGFLGRLLGRKRYFVLCIVAFTICSFLCGIATDLGQLIVFRVLQGLFGGGLQPNQQSIILDTFPPEQRNRAFSI
SAVAIVVAPVLGPTLGGWITDNFSWRWVFLLNVPIGVLTSLAVIQLVEDPPWKRGRARGLSIDYIGITLIAIGLGCLQVM
LDRGEDEDWFASTFIRTFAVLTVAGLVGATFWLLYAKKPVVDLSCLKDRNFALGCVTIATFAVVLYGSAVLVPQLAQQRL
GYTAMLAGLVLSPGALLITLEIPIVSKLMPYVQTRFLVCFGFLLLAASLVYSHTLVPDIDYPNLLKIRSAQSLAIGFLFV
PITTLAYLTVPRRLNDDASALFTMFRNVAGSIGISLSTALIRERAQARMAHLSTHLSPLSQNFDDTLRQNAQTIASLSGM
PPPAALQTASGQLYETFVSQATILAYLDVFAMLAIFCFVCTPLTLLFSPVKAAGGPGGH

Sequences:

>Translated_539_residues
MNGSTPTPPAAPRRDRNGNGDGTWRPAANPWLIAVVVTLAAFMEVLDTTIVNVALPHIAGTMSASYDEATWTLTSYLVAN
GIVLPISGFLGRLLGRKRYFVLCIVAFTICSFLCGIATDLGQLIVFRVLQGLFGGGLQPNQQSIILDTFPPEQRNRAFSI
SAVAIVVAPVLGPTLGGWITDNFSWRWVFLLNVPIGVLTSLAVIQLVEDPPWKRGRARGLSIDYIGITLIAIGLGCLQVM
LDRGEDEDWFASTFIRTFAVLTVAGLVGATFWLLYAKKPVVDLSCLKDRNFALGCVTIATFAVVLYGSAVLVPQLAQQRL
GYTAMLAGLVLSPGALLITLEIPIVSKLMPYVQTRFLVCFGFLLLAASLVYSHTLVPDIDYPNLLKIRSAQSLAIGFLFV
PITTLAYLTVPRRLNDDASALFTMFRNVAGSIGISLSTALIRERAQARMAHLSTHLSPLSQNFDDTLRQNAQTIASLSGM
PPPAALQTASGQLYETFVSQATILAYLDVFAMLAIFCFVCTPLTLLFSPVKAAGGPGGH
>Mature_539_residues
MNGSTPTPPAAPRRDRNGNGDGTWRPAANPWLIAVVVTLAAFMEVLDTTIVNVALPHIAGTMSASYDEATWTLTSYLVAN
GIVLPISGFLGRLLGRKRYFVLCIVAFTICSFLCGIATDLGQLIVFRVLQGLFGGGLQPNQQSIILDTFPPEQRNRAFSI
SAVAIVVAPVLGPTLGGWITDNFSWRWVFLLNVPIGVLTSLAVIQLVEDPPWKRGRARGLSIDYIGITLIAIGLGCLQVM
LDRGEDEDWFASTFIRTFAVLTVAGLVGATFWLLYAKKPVVDLSCLKDRNFALGCVTIATFAVVLYGSAVLVPQLAQQRL
GYTAMLAGLVLSPGALLITLEIPIVSKLMPYVQTRFLVCFGFLLLAASLVYSHTLVPDIDYPNLLKIRSAQSLAIGFLFV
PITTLAYLTVPRRLNDDASALFTMFRNVAGSIGISLSTALIRERAQARMAHLSTHLSPLSQNFDDTLRQNAQTIASLSGM
PPPAALQTASGQLYETFVSQATILAYLDVFAMLAIFCFVCTPLTLLFSPVKAAGGPGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=484, Percent_Identity=35.7438016528926, Blast_Score=314, Evalue=8e-87,
Organism=Escherichia coli, GI1788710, Length=508, Percent_Identity=34.251968503937, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1790195, Length=440, Percent_Identity=28.1818181818182, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI87081983, Length=438, Percent_Identity=25.7990867579909, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788392, Length=415, Percent_Identity=28.433734939759, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI87082320, Length=202, Percent_Identity=24.2574257425743, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=168, Percent_Identity=31.547619047619, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=152, Percent_Identity=30.2631578947368, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=152, Percent_Identity=32.2368421052632, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323735, Length=207, Percent_Identity=28.9855072463768, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58088; Mature: 58088

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00092 N6_MTASE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGSTPTPPAAPRRDRNGNGDGTWRPAANPWLIAVVVTLAAFMEVLDTTIVNVALPHIAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMSASYDEATWTLTSYLVANGIVLPISGFLGRLLGRKRYFVLCIVAFTICSFLCGIATDL
HHCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GQLIVFRVLQGLFGGGLQPNQQSIILDTFPPEQRNRAFSISAVAIVVAPVLGPTLGGWIT
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHCCCEEEHHHHHHHHHHHHCCCCCCCEE
DNFSWRWVFLLNVPIGVLTSLAVIQLVEDPPWKRGRARGLSIDYIGITLIAIGLGCLQVM
CCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
LDRGEDEDWFASTFIRTFAVLTVAGLVGATFWLLYAKKPVVDLSCLKDRNFALGCVTIAT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHH
FAVVLYGSAVLVPQLAQQRLGYTAMLAGLVLSPGALLITLEIPIVSKLMPYVQTRFLVCF
HHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHH
GFLLLAASLVYSHTLVPDIDYPNLLKIRSAQSLAIGFLFVPITTLAYLTVPRRLNDDASA
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFTMFRNVAGSIGISLSTALIRERAQARMAHLSTHLSPLSQNFDDTLRQNAQTIASLSGM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PPPAALQTASGQLYETFVSQATILAYLDVFAMLAIFCFVCTPLTLLFSPVKAAGGPGGH
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNGSTPTPPAAPRRDRNGNGDGTWRPAANPWLIAVVVTLAAFMEVLDTTIVNVALPHIAG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMSASYDEATWTLTSYLVANGIVLPISGFLGRLLGRKRYFVLCIVAFTICSFLCGIATDL
HHCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GQLIVFRVLQGLFGGGLQPNQQSIILDTFPPEQRNRAFSISAVAIVVAPVLGPTLGGWIT
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHCCCEEEHHHHHHHHHHHHCCCCCCCEE
DNFSWRWVFLLNVPIGVLTSLAVIQLVEDPPWKRGRARGLSIDYIGITLIAIGLGCLQVM
CCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
LDRGEDEDWFASTFIRTFAVLTVAGLVGATFWLLYAKKPVVDLSCLKDRNFALGCVTIAT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHH
FAVVLYGSAVLVPQLAQQRLGYTAMLAGLVLSPGALLITLEIPIVSKLMPYVQTRFLVCF
HHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHHHHHH
GFLLLAASLVYSHTLVPDIDYPNLLKIRSAQSLAIGFLFVPITTLAYLTVPRRLNDDASA
HHHHHHHHHHHHHCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFTMFRNVAGSIGISLSTALIRERAQARMAHLSTHLSPLSQNFDDTLRQNAQTIASLSGM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
PPPAALQTASGQLYETFVSQATILAYLDVFAMLAIFCFVCTPLTLLFSPVKAAGGPGGH
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]