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The map label for this gene is trmB [H]

Identifier: 126452731

GI number: 126452731

Start: 3014957

End: 3015754

Strand: Direct

Name: trmB [H]

Synonym: BURPS1106A_3091

Alternate gene names: 126452731

Gene position: 3014957-3015754 (Clockwise)

Preceding gene: 126453435

Following gene: 126455007

Centisome position: 75.59

GC content: 70.55

Gene sequence:

>798_bases
ATGATCCACGACGACGACCCGAACGCCCCCGGCGCGCCGCACGACGACGACGCCACCGCCGCCCCCGCCTCGGCAACCCG
CGCCGCGCCGGCCGCCGGTGACGACGACGACGCGAACCCGCTCCATCTGCGCCGCATCCGCAGCTTCGTCACGCGCGCCG
GCCGCGTATCCACCGGCCAGCGCCGCGCGATCGACGAGCTCGGCCCGCGCTTCGTCATCCCGTACGGCAGCGCGCAGCCG
GACTGGGACGCCATCTTCGACCGCCGCGCGCCGCGCGTGCTGGAAATCGGCTTCGGGATGGGCGCGTCGACGGCGGAAAT
CGCCGCGCTGCGCCCCGGCGACGACTTCATCGGCGTCGAGGTGCACGAGCCGGGCGTCGGCGCGTTGCTGAAGCTGATCG
GCGAGCAGCAACTGTCGAACATCCGGATCATTCAGCACGACGCGGTCGAGGTGCTCGCGCAGATGATCGCGCCCGACAGC
CTCGACGGCGTGCACATCTTCTTCCCGGACCCGTGGCACAAGGCGCGCCATCACAAGCGCCGGCTGATCCAGCCGCCGTT
CGTCGCGCAGCTCGCGGCGCATCTCAAACCCGGCGCATACCTGCATTGCGCAACCGACTGGCAGAACTACGCGGAACAGA
TGCTCGAGGTGCTGAGCGCCGACCCGTCGCTCGAAAACACCGCGCAAGACTATGCGCCCCGCCCCGGCTATCGTCCGGTG
ACGAAGTTCGAGCGCCGCGGCCTGCGGCTCGGGCACGGCGTATGGGATCTCGTGTTCAGAAAGAAGCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
TCGTATTTCATACGCAGTAAGGGCGGCCCGTCTCGAATGCGGCGAATGCGACGCCGTCGCGCACCGCGCCTGCGGCCCGC
CCCTCGCCTTCATTGTCACG

Downstream 100 bases:

>100_bases
CGCGCGACGCCGCGCGCATTACGCGCGGCGTCGGCCGGCATGCCCACCGGCGAACCGAAAGCCCGCAACGGCGGCGGCGG
CAGCGCGCTCACCGCCGCCG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVIPYGSAQP
DWDAIFDRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDS
LDGVHIFFPDPWHKARHHKRRLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
TKFERRGLRLGHGVWDLVFRKKRAG

Sequences:

>Translated_265_residues
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVIPYGSAQP
DWDAIFDRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDS
LDGVHIFFPDPWHKARHHKRRLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
TKFERRGLRLGHGVWDLVFRKKRAG
>Mature_265_residues
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQRRAIDELGPRFVIPYGSAQP
DWDAIFDRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVEVHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDS
LDGVHIFFPDPWHKARHHKRRLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
TKFERRGLRLGHGVWDLVFRKKRAG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=229, Percent_Identity=53.2751091703057, Blast_Score=246, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 29179; Mature: 29179

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQ
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
RRAIDELGPRFVIPYGSAQPDWDAIFDRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVE
HHHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHEEEECCCCCEEEEE
VHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDSLDGVHIFFPDPWHKARHHKR
ECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
RLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
HCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCH
TKFERRGLRLGHGVWDLVFRKKRAG
HHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MIHDDDPNAPGAPHDDDATAAPASATRAAPAAGDDDDANPLHLRRIRSFVTRAGRVSTGQ
CCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCH
RRAIDELGPRFVIPYGSAQPDWDAIFDRRAPRVLEIGFGMGASTAEIAALRPGDDFIGVE
HHHHHHCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCCCCCCCHHEEEECCCCCEEEEE
VHEPGVGALLKLIGEQQLSNIRIIQHDAVEVLAQMIAPDSLDGVHIFFPDPWHKARHHKR
ECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHH
RLIQPPFVAQLAAHLKPGAYLHCATDWQNYAEQMLEVLSADPSLENTAQDYAPRPGYRPV
HCCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCH
TKFERRGLRLGHGVWDLVFRKKRAG
HHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA