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The map label for this gene is ytwF [H]

Identifier: 126452692

GI number: 126452692

Start: 3021757

End: 3022086

Strand: Direct

Name: ytwF [H]

Synonym: BURPS1106A_3100

Alternate gene names: 126452692

Gene position: 3021757-3022086 (Clockwise)

Preceding gene: 126451653

Following gene: 126453909

Centisome position: 75.76

GC content: 68.79

Gene sequence:

>330_bases
GTGCCGATGCAGATCCTGAGCGCTTCCGCCCTCGCCGAATGGCTGCGCGACCCGGCGCGCCCGGCGCCCATCGTGCTCGA
CGTGCGCGAGCCGTGGGAGATCGCGACCGCGAAGATCGACGGCAGCGTGTCGATCCCGATGCAGCAGATTCCCGCGCGCA
GCGAGGAGCTCGACGACGAAGCGGCAATCGTCTGCGTGTGCCATCACGGGATGCGCAGCGCGCAGGTCGCGATGTTCCTC
GAATCGCGCGGCTTCACGAAGCTCTACAACCTGCAAGGCGGGATCGACGCGTGGTCGCGCGACGTCGATCCCGCGGTGCC
GCGCTACTGA

Upstream 100 bases:

>100_bases
TCGACGACACGCAATATCGCGTCGCCGACGTATTCGAGACCTACGTCGATCACCTCGTCAACGCGATCGAGCCGTCGCGC
TTCAAGTTCTGAGGCCGCGC

Downstream 100 bases:

>100_bases
GCGCGCATCGCGGCGGCGCGCGCGACGCGCGCCGCTTCGCCCGGGCGCGCCGCCCGTTTCCCTGAGCGCGGCGCCCGTTT
GCCGCGCTCCGCCTACTCCG

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 108

Protein sequence:

>109_residues
MPMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDEAAIVCVCHHGMRSAQVAMFL
ESRGFTKLYNLQGGIDAWSRDVDPAVPRY

Sequences:

>Translated_109_residues
MPMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDEAAIVCVCHHGMRSAQVAMFL
ESRGFTKLYNLQGGIDAWSRDVDPAVPRY
>Mature_108_residues
PMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDEAAIVCVCHHGMRSAQVAMFLE
SRGFTKLYNLQGGIDAWSRDVDPAVPRY

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 12076; Mature: 11945

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDE
CCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCEECCHHHCCCCCCCCCCC
AAIVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPAVPRY
CEEEEEECCCCCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PMQILSASALAEWLRDPARPAPIVLDVREPWEIATAKIDGSVSIPMQQIPARSEELDDE
CHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCEECCHHHCCCCCCCCCCC
AAIVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPAVPRY
CEEEEEECCCCCHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]