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The map label for this gene is cyaE [H]

Identifier: 126452663

GI number: 126452663

Start: 3543888

End: 3545303

Strand: Direct

Name: cyaE [H]

Synonym: BURPS1106A_3644

Alternate gene names: 126452663

Gene position: 3543888-3545303 (Clockwise)

Preceding gene: 126453800

Following gene: 126453820

Centisome position: 88.85

GC content: 68.79

Gene sequence:

>1416_bases
ATGGCCGGCGCGCTCGCGCTCTTCGCATCGCAAGCGGCGTACGCGCAATGGCTCGATCTCTACGGAACCCGAAAGAATGT
GTCGGCTTCTCCCGCCGCGCCGATGCTGAAGAACGCGACATGCCAGCCCGAGCTCGCGGACCGTCCGATCGAGCTCGAAG
ACGCGATCCTGCAGGCGATCTGCGCGAACCCGCAGGCACGGCGCGCGTGGGCCGATGCGCGCGCGCAGGCCGCCCAGCTC
GGCGTGAAGGAGGCGGCGTATCTGCCGACGCTCAATGCAACAGCGGGCATTCAGCGCAACTGGCAAACGACGACGTATGA
GCAGGACCTCGGCCCGATCACGCTCACCGCCGACCAGAAGCAGATCCAGACGAGCCGCTACGGCGCGTTGAACCTGAGCT
GGGTGCTGTTCGATTTCGGCAAGCGCAGCGCGGCGCTCAGGCAGGCGCGCGAGCTCCTCGCCGCCGCCAACGCGACGCAG
GACGACACGCTCCAGACCATCCTGTTCAATGCCGCCCAGGCGTATTACAACCTGCGCGACACGCAGGCGGCGCTCGACGC
GGTGCGCACGACCGAACGCGTCGCGCAGGATAGCCTCACCGCGGCGAAGGCGAAGCACGACGCCGGCGCCGGCACGCTGA
GCGATCAACTGCAGGCGCAAACGAGCTATCGCCGCGCGGTGCTCGATCGCGTGAGCGCCGAAGGCGACGTGCGGAACGCG
ACGGGCGTGCTCGCCGTCGCGATGGGGCTCGACGCGAACACGCCGCTGCGGATCGCGACCGCCGAGCCGCCGGTCGATCG
CAACGCGTTCGCCGAAGGCATCGCGCAATTGATCGACGAGGCGAAGCGGCAGCATCCGAAGCTCGTCGCCGCGCGCGCGA
AACTCGACGCGGCGCGCGCGAACGTCGATGCGGTGCGTGCGCAGGGCCGCCCGACCATCTCGCTGACCGGCAGCCTCAGC
CGCAACAGCCCGTCGTATCAGCAGCAGCCGGGCATCCAGACCACCGCGAGCCACGGCAGCATGATCGGCGTGCAGGTGAC
GATCCCGCTCTTCGAAGGCTTCGCGTCCGGCTACCGCGTCGCGGCCGCGCAGGCACAGGCGGATGCGCAGGAGGCCGACG
TGCAGAACACCGAGCTGAACGTCTCGCTCGACGTGTGGAAGAGCTATCAGAGCCTGCAGACCGACACCGCGAATCTCGAC
AATTCGAAGGACCTGCTCGGCGATGCGCAACGCTCGCTCGACATCGCACGGGGCCGGTACAAGGCGGGCGTCGGCACGTT
CACGGAGTTGCTCAATGCGCAGACCGCGCTCGCGGACGCGCAGAAGCAACGCGTGCAGGCGCTGTCGAAATGGCGCACCG
CGCGGCTCAAGCTCGCGGCGAGCCTCGGCAGCATCGGCCTGTGGTCCGCCCATTGA

Upstream 100 bases:

>100_bases
CCGCTGCGGCCTGCGCCGCTCCCGCCGCGCCGCCCGCGCAAGCCGCTACGCAACCGGCCACGGGGCGATGATCGCGATGA
AGCCCGCACGCACCCTCGCC

Downstream 100 bases:

>100_bases
ACGATGCGCGAACACAAAGGCGAAGGCGAACGCTCCTCGCGCTCGCGCACGGAACGATGAAGCGACCGGCGAAGGCGGCC
CATGCAGCGACGCCCGCCGC

Product: TolC family type I secretion outer membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAGALALFASQAAYAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRAWADARAQAAQL
GVKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQ
DDTLQTILFNAAQAYYNLRDTQAALDAVRTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVSAEGDVRNA
TGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQGRPTISLTGSLS
RNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLD
NSKDLLGDAQRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWSAH

Sequences:

>Translated_471_residues
MAGALALFASQAAYAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRAWADARAQAAQL
GVKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQ
DDTLQTILFNAAQAYYNLRDTQAALDAVRTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVSAEGDVRNA
TGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQGRPTISLTGSLS
RNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLD
NSKDLLGDAQRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWSAH
>Mature_470_residues
AGALALFASQAAYAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRAWADARAQAAQLG
VKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQD
DTLQTILFNAAQAYYNLRDTQAALDAVRTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVSAEGDVRNAT
GVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQGRPTISLTGSLSR
NSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLDN
SKDLLGDAQRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWSAH

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=423, Percent_Identity=21.9858156028369, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50485; Mature: 50354

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGALALFASQAAYAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAI
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHCCCCCCHHHHHHHHH
CANPQARRAWADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQK
HCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHH
QIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQDDTLQTILFNAAQAYYNLRD
HHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TQAALDAVRTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVSAEGDVRNA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARA
CCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NVDAVRAQGRPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRV
CHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEEEEEHHHHHHHCCCEE
AAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLDNSKDLLGDAQRSLDIARGRY
EHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHH
KAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWSAH
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AGALALFASQAAYAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHCCCCCCHHHHHHHHH
CANPQARRAWADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQK
HCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHH
QIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQDDTLQTILFNAAQAYYNLRD
HHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
TQAALDAVRTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLDRVSAEGDVRNA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARA
CCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
NVDAVRAQGRPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRV
CHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEEEEEHHHHHHHCCCEE
AAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLDNSKDLLGDAQRSLDIARGRY
EHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCHH
KAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWSAH
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]