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The map label for this gene is nagE [H]

Identifier: 126452617

GI number: 126452617

Start: 524319

End: 526079

Strand: Direct

Name: nagE [H]

Synonym: BURPS1106A_0555

Alternate gene names: 126452617

Gene position: 524319-526079 (Clockwise)

Preceding gene: 126451463

Following gene: 126452283

Centisome position: 13.15

GC content: 69.73

Gene sequence:

>1761_bases
ATGGACGGAAATCCGTTTCTGAAAATACAGAGCCTCGGCAGGGCGCTGATGCTGCCGATCGCGGTGCTGCCGGTGGCGGG
CATCCTGCTGCGCCTCGGGCAGCAGGACGTGCTCAACATCAAGATGATCGCCGACGCGGGCGGCGCGATCTTCGAGAACC
TGCCGCTGCTGTTCGCGATCGGCGTCGCGGTCGGCTTCGCGAAGGACAACAACGGCGTGGCGGCGCTCGCGGGCGCGATC
GGCTATCTGATCGAAGTCGCGATCATGAAGGACATCGATCCGAAGCTGAACATGGGCGTGCTGTCCGGGATCATCGCGGG
CGTCGTCGCGGGGCTGCTGTACAACCGCTACAAGGACATCAAGCTGCCCGACTACCTCGCGTTCTTCGGCGGCAAGCGCT
TCGTGCCGATCATCACGGGGCTCGCGTGCGTCGTGCTCGGGATCGTGTTCGGCTACGTATGGCAGCCGGTGCAGCACGCG
ATCGACGCGGTCGGCCAGTGGCTGCTGACGGCGGGCGCGATCGGCACGTTCGTCTACGGGTTCCTGAACCGCCTGCTGCT
CGTCACGGGGCTGCACCACATCATCAATTCGCTCGTCTGGTTCGTGTTCGGCACGTTCACGCCGGCGGGCGGCGCCGCGG
TGACGGGCGATCTGCATCGCTTCTTCGCGGGCGATCCGAGCGCGGGCGGCTTCATGGCGGGCTTCTTCCCGATCATGATG
TTCGGCCTGCCGGCCGCGTGCCTCGCGATGTTTCACGAGGCGCCGAAGGCGCGCCGCGCGATCGTCGGCGGCCTGCTGTT
CTCGATGGCGCTCACCTCGTTCCTGACGGGCGTGACCGAGCCGATCGAGTTCAGCTTCATGTTCCTCGCGCCGGTGCTGT
ACGTGATCCACGCGGTGCTCACGGGCCTTTCGCTCGCGATCTGCCAGTTGCTCGGCGTGAAGCTCGGCTTCACGTTCTCG
GCGGGCGCGATCGACTATGTGCTGAACTACGGGCTGTCGACGAAGGGCTGGATCGCGATCCCGCTCGGCCTTGCGTACGG
TCTCGCCTACTACGGCCTCTTCCGCTTCTTCATCCGCAAGTTCAACATGGCGACGCCGGGCCGCGAGCCCGCGGGCGCGG
ACGCGCAGGCGCAGTCGTTCGCGTCGGGCGGTTTCGTCGCGCCGACGGCGGGCGCATCGGTGCCGCGCGCGCAGCGCTAC
ATCGCGGCGCTCGGCGGCGCGGCGAACCTGTCGGTCGTCGATGCGTGCACGACGCGGCTGCGTCTTTCCGTCGTCGATCC
CGAGAAGGTGTCCGAAGCGGATCTGCGCACGATCGGCGCGCGCGGCGTGCTCAAACGCGGCGGCAGCAGCGTGCAGGTGA
TCATCGGGCCGGAGGCGGACCTCATCGCCGATGAGATTCGCGCGACGCTCGGCAGCGGCGCGGCGGCGCCCGCGGCTGCG
GCTGCCGCGGCGCCTGCGGCGGCGGCAACGGCGGCGGGCGCGCAGTCGGGCCCGCTCGATCCGGAGCCGACGCGCTGGCT
CGCGGTGTTCGGCGGCGCGACGAACGTCGCTTCGCTCGACGCGGTCGCGGCGACGCGCCTGCGCGTCGTCGTGCGCGATC
CGTCGGCGGTCGATCGCCAGCGCCTCGCGACGCTTGACGTCGCCTGGGTCGCGAGCGACACGTTCCATATCGTCTGCGGC
CAGTCGGCGCCGCGCTATGCGCAGCAGCTCGCCGCGCGCCTGCCGTCGTCCGACGGCGGCACGGCGGCCCAGCCCGCCTG
A

Upstream 100 bases:

>100_bases
GTCCGGCGAATCGCGCGCGTGGCAAGCGCGTTTCAGGTGTCAATCAACAGAGCTGATCAATTATTCAACGACGAGACACG
ATAAAGGATTGGAGGATCGA

Downstream 100 bases:

>100_bases
CGCGGCGACGCGGCCGGCCGAATCCGCGAGGCGGATTCGGCTTCGGTCCGGATGCGGGGAACACGAAAAACGGCTTGCGG
ATATCGCAGGCCGTTTTTCC

Product: PTS system, N-acetylglucosamine-specific IIBC component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQQDVLNIKMIADAGGAIFENLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLACVVLGIVFGYVWQPVQHA
IDAVGQWLLTAGAIGTFVYGFLNRLLLVTGLHHIINSLVWFVFGTFTPAGGAAVTGDLHRFFAGDPSAGGFMAGFFPIMM
FGLPAACLAMFHEAPKARRAIVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQLLGVKLGFTFS
AGAIDYVLNYGLSTKGWIAIPLGLAYGLAYYGLFRFFIRKFNMATPGREPAGADAQAQSFASGGFVAPTAGASVPRAQRY
IAALGGAANLSVVDACTTRLRLSVVDPEKVSEADLRTIGARGVLKRGGSSVQVIIGPEADLIADEIRATLGSGAAAPAAA
AAAAPAAAATAAGAQSGPLDPEPTRWLAVFGGATNVASLDAVAATRLRVVVRDPSAVDRQRLATLDVAWVASDTFHIVCG
QSAPRYAQQLAARLPSSDGGTAAQPA

Sequences:

>Translated_586_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQQDVLNIKMIADAGGAIFENLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLACVVLGIVFGYVWQPVQHA
IDAVGQWLLTAGAIGTFVYGFLNRLLLVTGLHHIINSLVWFVFGTFTPAGGAAVTGDLHRFFAGDPSAGGFMAGFFPIMM
FGLPAACLAMFHEAPKARRAIVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQLLGVKLGFTFS
AGAIDYVLNYGLSTKGWIAIPLGLAYGLAYYGLFRFFIRKFNMATPGREPAGADAQAQSFASGGFVAPTAGASVPRAQRY
IAALGGAANLSVVDACTTRLRLSVVDPEKVSEADLRTIGARGVLKRGGSSVQVIIGPEADLIADEIRATLGSGAAAPAAA
AAAAPAAAATAAGAQSGPLDPEPTRWLAVFGGATNVASLDAVAATRLRVVVRDPSAVDRQRLATLDVAWVASDTFHIVCG
QSAPRYAQQLAARLPSSDGGTAAQPA
>Mature_586_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQQDVLNIKMIADAGGAIFENLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIEVAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLACVVLGIVFGYVWQPVQHA
IDAVGQWLLTAGAIGTFVYGFLNRLLLVTGLHHIINSLVWFVFGTFTPAGGAAVTGDLHRFFAGDPSAGGFMAGFFPIMM
FGLPAACLAMFHEAPKARRAIVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQLLGVKLGFTFS
AGAIDYVLNYGLSTKGWIAIPLGLAYGLAYYGLFRFFIRKFNMATPGREPAGADAQAQSFASGGFVAPTAGASVPRAQRY
IAALGGAANLSVVDACTTRLRLSVVDPEKVSEADLRTIGARGVLKRGGSSVQVIIGPEADLIADEIRATLGSGAAAPAAA
AAAAPAAAATAAGAQSGPLDPEPTRWLAVFGGATNVASLDAVAATRLRVVVRDPSAVDRQRLATLDVAWVASDTFHIVCG
QSAPRYAQQLAARLPSSDGGTAAQPA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=467, Percent_Identity=54.389721627409, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1787343, Length=486, Percent_Identity=44.238683127572, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1787908, Length=520, Percent_Identity=36.7307692307692, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 60839; Mature: 60839

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHEEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHEEEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1745234 [H]