Click here to switch to the map view.

The map label for this gene is bpeA [H]

Identifier: 126452582

GI number: 126452582

Start: 838555

End: 839817

Strand: Direct

Name: bpeA [H]

Synonym: BURPS1106A_0859

Alternate gene names: 126452582

Gene position: 838555-839817 (Clockwise)

Preceding gene: 126453448

Following gene: 126453664

Centisome position: 21.02

GC content: 69.83

Gene sequence:

>1263_bases
ATGCGCGTCGAACGGGTTCCATACCGCTTAATCACTGTCGCGACGGCTGCCGTTTTCCTGGCCGCGTGCGGAAAAAAAGA
ATCGGCTCCGCCCCCTCAAACGCCCGAAGTCGGCGTCGTCACCGTCCAGCCGCAGCCCGTGCCGGTCGTCTCCGAACTGC
CGGGCCGTACGAGCGCCTATCTGGTCGCGCAGGTGCGCGCACGGGTCGACGGCATCGTGTTGCGGCGCGAGTTCACGGAA
GGCAGCGACGTCAAGGCCGGCCAGCGCCTGTACAAGATCGATCCGGCACCCTATATCGCGCAATTGAACAGCGCGAAGGC
GACGCTCGCGAAGGCGCAGGCGAACCTCGCGACGCAGAACGCGCTCGTCGCGCGCTACAAGGTGCTCGTCGCCGCGAACG
CGGTCAGCAAGCAGCAGTACGACGATGCGGTGGCCGCGCAAGGGCAGGCGGCCGCCGACGTGGGCGCGGGCAAGGCCGCC
GTCGAGACCGCGCAGATCAACCTCGGCTATACGGATGTCGTCTCGCCGATCACGGGCCGCGTCGGCATCTCGCAGGTCAC
GCCGGGCGCGTACGTGCAGGCGAGCCAGGCGACGCTGATGTCGACCGTCCAGCAGCTCGATCCGGTCTACGTCGATCTCA
CGCAGTCGAGCCTCGACGGCCTGAAGCTGCGCCAGGACATCCAGAGCGGGCGCATCAAGACGGAAGGCCCGGGCGCGGCG
AAGGTCACGCTGATTCTCGAGGACGGCAAGCCGTACCCGGAGCGGGGCAAGCTGCAGTTCAGCGATGTCACGGTCGACCA
GACGACGGGCTCGGTCACGATCCGCGCGATCTTCCCGAACAAGCAGCGCGTGCTGCTGCCGGGCATGTTCGTGCGCGCGC
GCATCGAAGAGGGCATCAACGAGAACGCGTTCCTCGTTCCGCAGATCGGCGTCACGCACGATCCGAAGGGCCAGGCGATC
GCGATGATCGTCGACGGCAAGGGCAAGGTCGAGCCGCGCGTGCTGGTCACGGGCGGCACGCAGGGCCAGAACTGGGTGGT
CGAGAGCGGCCTGCAGGCGGGCGACCGCGTGATCGTGCAGGGCATCGACAAGGTGCGCCCGGGCATGACCGTGAAGGCCG
CCGAGGCTCAACTGCCGGCCGCGGCGGCCGGCGCGTCGGGTGCCGCGCCCGCGGGCGGCTCGCCCGCCCAGGCCGCGGCG
GCGAGCGCGGCCGCGTCGGGTGCCGCGCCGTCGAGCGCTGCCGCCGCGTCGAGCGCGCAATAA

Upstream 100 bases:

>100_bases
AGTCAAGCAATTCAGTTGTCCGATCCGCGCCGCAAGGCGCGTTCGTGTTGTGTCCCCGAGGCCGGCACTTATTTCATCTA
GTTACAAACGAGGTCGCTCC

Downstream 100 bases:

>100_bases
CAGGGGGCCTGTTTCATGGCAAAGTTTTTTATCGATCGCCCGATCTTCGCGTGGGTGATCGCCATCATCCTGATGCTGGC
CGGCGTCGCGGCGATCTTCA

Product: multidrug efflux periplasmic linker protein BpeA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQPVPVVSELPGRTSAYLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQQYDDAVAAQGQAAADVGAGKAA
VETAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
KVTLILEDGKPYPERGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGINENAFLVPQIGVTHDPKGQAI
AMIVDGKGKVEPRVLVTGGTQGQNWVVESGLQAGDRVIVQGIDKVRPGMTVKAAEAQLPAAAAGASGAAPAGGSPAQAAA
ASAAASGAAPSSAAAASSAQ

Sequences:

>Translated_420_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQPVPVVSELPGRTSAYLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQQYDDAVAAQGQAAADVGAGKAA
VETAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
KVTLILEDGKPYPERGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGINENAFLVPQIGVTHDPKGQAI
AMIVDGKGKVEPRVLVTGGTQGQNWVVESGLQAGDRVIVQGIDKVRPGMTVKAAEAQLPAAAAGASGAAPAGGSPAQAAA
ASAAASGAAPSSAAAASSAQ
>Mature_420_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQPVPVVSELPGRTSAYLVAQVRARVDGIVLRREFTE
GSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQNALVARYKVLVAANAVSKQQYDDAVAAQGQAAADVGAGKAA
VETAQINLGYTDVVSPITGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
KVTLILEDGKPYPERGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGINENAFLVPQIGVTHDPKGQAI
AMIVDGKGKVEPRVLVTGGTQGQNWVVESGLQAGDRVIVQGIDKVRPGMTVKAAEAQLPAAAAGASGAAPAGGSPAQAAA
ASAAASGAAPSSAAAASSAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=385, Percent_Identity=53.7662337662338, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1789665, Length=362, Percent_Identity=51.1049723756906, Blast_Score=348, Evalue=3e-97,
Organism=Escherichia coli, GI1789929, Length=365, Percent_Identity=50.4109589041096, Blast_Score=336, Evalue=2e-93,
Organism=Escherichia coli, GI87082036, Length=361, Percent_Identity=30.4709141274238, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43298; Mature: 43298

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQPVPVVSELPGRTSAY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQQYDDAVAAQGQAAADVGAGKAAVETAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEECCCCCE
KVTLILEDGKPYPERGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGIN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHCCCC
ENAFLVPQIGVTHDPKGQAIAMIVDGKGKVEPRVLVTGGTQGQNWVVESGLQAGDRVIVQ
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEE
GIDKVRPGMTVKAAEAQLPAAAAGASGAAPAGGSPAQAAAASAAASGAAPSSAAAASSAQ
CHHHHCCCCEEEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQPVPVVSELPGRTSAY
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCHHH
LVAQVRARVDGIVLRREFTEGSDVKAGQRLYKIDPAPYIAQLNSAKATLAKAQANLATQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHCCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQQYDDAVAAQGQAAADVGAGKAAVETAQINLGYTDVVSPITGR
HHHHHEEEEEEECHHHHHHHHHHHHCCCCCHHHCCCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLDGLKLRQDIQSGRIKTEGPGAA
CCCCCCCCCCEEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEECCCCCE
KVTLILEDGKPYPERGKLQFSDVTVDQTTGSVTIRAIFPNKQRVLLPGMFVRARIEEGIN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEECCHHHHHHHHCCCC
ENAFLVPQIGVTHDPKGQAIAMIVDGKGKVEPRVLVTGGTQGQNWVVESGLQAGDRVIVQ
CCEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEE
GIDKVRPGMTVKAAEAQLPAAAAGASGAAPAGGSPAQAAAASAAASGAAPSSAAAASSAQ
CHHHHCCCCEEEECCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]