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The map label for this gene is livK [C]

Identifier: 126452565

GI number: 126452565

Start: 1943286

End: 1944584

Strand: Reverse

Name: livK [C]

Synonym: BURPS1106A_1977

Alternate gene names: 126452565

Gene position: 1944584-1943286 (Counterclockwise)

Preceding gene: 126453473

Following gene: 126453202

Centisome position: 48.76

GC content: 69.21

Gene sequence:

>1299_bases
GTGCGCGACGCTCGCCGCACCGCGCACGGCGCATCACGAGAACCCGAACGCGCGGCGAGTTTCCCGACGGGCGCGCCCAC
TGTGTCGATCCATGCCGAAAGCGATTGGGCATCCGAACCGATCGATTCGTTTGAGGCCGCCCGGCAATCGAAAATCGTGA
CGTTTTCTCGAGCGAGGGTCGACATGAACGTTCGCATGGGGTGTTTATCGCTGCTGGCCGCCGTCGCATTCGACGCGCAC
GCGCAATCATCGGTCGTCCGGATCGGCGTCGCGATGCCGCTGACGGGGCCCGTCGCCCATCTGGGCAAGGACGTGCAGAA
CGGCGCGCAGCTCGCCGTCGACGAGCTGAACCGCGCGCCGCCGACGATCGACGGCAAGCCGGTGAAGTTCGTGCTCGTCG
TCGAAGACGATCAGGGCGATCCACGGCAGGCGGTGCAGGTCGCGCAGCGCCTCGTCGACGCGCGGGTCGCGGGCGTCGTC
GGCGATCTGAACTCGGGGCCGACGATCGTCGCGGCGAAGGTCTACGCGGCGGCCGGCATCGCGCAGATCGCGCCCGCCGC
GACCCATTCCGCGTACACGCAGCTAGGCTACAAGACCGCGTTCCGGCTGATGGCGACCGACAACCAGCAAGGCGCGTCGC
TCGCCGGGCTCGCCGCGAAGCTCGCGAAAGGCCGGCCGATCGCGCTGATCGACGATCGCGGCGCCTACGGCCAGGGCCTC
ATCGATCAGACGGAGAAGACACTGCGCGCGAGCGGCGTCACGCGAATCATCCGCGACTACACGACGGACACCGCGGTCAA
TTTCGCGTCGATTCTCACGCGCGTGAAGGGCGCGCATGCGGCCGTGATCGTCTACGGCGGCGCCGATGCACAGGCGGGGC
CGATGGTGCGGCAGATGAAGGCGCTCGGAATCGACGCGGCGTTCGTCGGCAGCGACGGCGTGTGCACCGGGCAGTGGACG
GCGCTGTCGTCGGGCGCGAACGAAGGCCAGTTCTGCACGCAAGCAGGCGACCCGCGCGCGAGGATGGCGGGTTACGCGGC
GTTCGAGCGGCGTTTCGAGGCGCGCTACGGCAAGGTGATCGTGTTCGCGCCGTATGGCTACGACGCGGTGATGCTGCTCG
CCGACGCGATGCGGCGCGCGAATTCGACCGAGCCGGCCGCTTTGCTCGGCGCGCTCGCGACGACCCGTTACGACGGCGTG
ATCGGCCGCATCCGCTTCAGTCCGCAGGGCGACAACCTGAACGGCGCGGTGACGGTCTACCGCGTGCAGCGCGGCGCGCT
CGTGCCGGTGTCCGACTGA

Upstream 100 bases:

>100_bases
ATCGGACGCGAGAGCGAGCGCATCGCGATCGTCGGGTGGCAGCGCCTGCTCGACGGCGCTTGAGCGCCCGTTTCATCGCC
CATTCCGAGGGCCTGGCTTC

Downstream 100 bases:

>100_bases
CACGCGATGCGGCGGCCGCGGAAGACGCGCGCGCCGCTTTGCCGAAGGCGCCGTTCGCGGCTCGGCAAGACCGGTTTGCC
GGACGCGCGCCCGTTCGCCG

Product: putative transporter, periplasmic substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MRDARRTAHGASREPERAASFPTGAPTVSIHAESDWASEPIDSFEAARQSKIVTFSRARVDMNVRMGCLSLLAAVAFDAH
AQSSVVRIGVAMPLTGPVAHLGKDVQNGAQLAVDELNRAPPTIDGKPVKFVLVVEDDQGDPRQAVQVAQRLVDARVAGVV
GDLNSGPTIVAAKVYAAAGIAQIAPAATHSAYTQLGYKTAFRLMATDNQQGASLAGLAAKLAKGRPIALIDDRGAYGQGL
IDQTEKTLRASGVTRIIRDYTTDTAVNFASILTRVKGAHAAVIVYGGADAQAGPMVRQMKALGIDAAFVGSDGVCTGQWT
ALSSGANEGQFCTQAGDPRARMAGYAAFERRFEARYGKVIVFAPYGYDAVMLLADAMRRANSTEPAALLGALATTRYDGV
IGRIRFSPQGDNLNGAVTVYRVQRGALVPVSD

Sequences:

>Translated_432_residues
MRDARRTAHGASREPERAASFPTGAPTVSIHAESDWASEPIDSFEAARQSKIVTFSRARVDMNVRMGCLSLLAAVAFDAH
AQSSVVRIGVAMPLTGPVAHLGKDVQNGAQLAVDELNRAPPTIDGKPVKFVLVVEDDQGDPRQAVQVAQRLVDARVAGVV
GDLNSGPTIVAAKVYAAAGIAQIAPAATHSAYTQLGYKTAFRLMATDNQQGASLAGLAAKLAKGRPIALIDDRGAYGQGL
IDQTEKTLRASGVTRIIRDYTTDTAVNFASILTRVKGAHAAVIVYGGADAQAGPMVRQMKALGIDAAFVGSDGVCTGQWT
ALSSGANEGQFCTQAGDPRARMAGYAAFERRFEARYGKVIVFAPYGYDAVMLLADAMRRANSTEPAALLGALATTRYDGV
IGRIRFSPQGDNLNGAVTVYRVQRGALVPVSD
>Mature_432_residues
MRDARRTAHGASREPERAASFPTGAPTVSIHAESDWASEPIDSFEAARQSKIVTFSRARVDMNVRMGCLSLLAAVAFDAH
AQSSVVRIGVAMPLTGPVAHLGKDVQNGAQLAVDELNRAPPTIDGKPVKFVLVVEDDQGDPRQAVQVAQRLVDARVAGVV
GDLNSGPTIVAAKVYAAAGIAQIAPAATHSAYTQLGYKTAFRLMATDNQQGASLAGLAAKLAKGRPIALIDDRGAYGQGL
IDQTEKTLRASGVTRIIRDYTTDTAVNFASILTRVKGAHAAVIVYGGADAQAGPMVRQMKALGIDAAFVGSDGVCTGQWT
ALSSGANEGQFCTQAGDPRARMAGYAAFERRFEARYGKVIVFAPYGYDAVMLLADAMRRANSTEPAALLGALATTRYDGV
IGRIRFSPQGDNLNGAVTVYRVQRGALVPVSD

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=344, Percent_Identity=30.8139534883721, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994941, Length=355, Percent_Identity=27.887323943662, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 45401; Mature: 45401

Theoretical pI: Translated: 9.40; Mature: 9.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDARRTAHGASREPERAASFPTGAPTVSIHAESDWASEPIDSFEAARQSKIVTFSRARV
CCCCHHHHCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEEEEEE
DMNVRMGCLSLLAAVAFDAHAQSSVVRIGVAMPLTGPVAHLGKDVQNGAQLAVDELNRAP
ECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHCHHHHCCHHHHHHHHCCCC
PTIDGKPVKFVLVVEDDQGDPRQAVQVAQRLVDARVAGVVGDLNSGPTIVAAKVYAAAGI
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHH
AQIAPAATHSAYTQLGYKTAFRLMATDNQQGASLAGLAAKLAKGRPIALIDDRGAYGQGL
HHHCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
IDQTEKTLRASGVTRIIRDYTTDTAVNFASILTRVKGAHAAVIVYGGADAQAGPMVRQMK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHH
ALGIDAAFVGSDGVCTGQWTALSSGANEGQFCTQAGDPRARMAGYAAFERRFEARYGKVI
HHCCCEEEECCCCCCCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCEE
VFAPYGYDAVMLLADAMRRANSTEPAALLGALATTRYDGVIGRIRFSPQGDNLNGAVTVY
EEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEE
RVQRGALVPVSD
EEECCCEEECCC
>Mature Secondary Structure
MRDARRTAHGASREPERAASFPTGAPTVSIHAESDWASEPIDSFEAARQSKIVTFSRARV
CCCCHHHHCCCCCCCHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEEEEEE
DMNVRMGCLSLLAAVAFDAHAQSSVVRIGVAMPLTGPVAHLGKDVQNGAQLAVDELNRAP
ECHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHCHHHHCCHHHHHHHHCCCC
PTIDGKPVKFVLVVEDDQGDPRQAVQVAQRLVDARVAGVVGDLNSGPTIVAAKVYAAAGI
CCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHH
AQIAPAATHSAYTQLGYKTAFRLMATDNQQGASLAGLAAKLAKGRPIALIDDRGAYGQGL
HHHCCHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCC
IDQTEKTLRASGVTRIIRDYTTDTAVNFASILTRVKGAHAAVIVYGGADAQAGPMVRQMK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHH
ALGIDAAFVGSDGVCTGQWTALSSGANEGQFCTQAGDPRARMAGYAAFERRFEARYGKVI
HHCCCEEEECCCCCCCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCEE
VFAPYGYDAVMLLADAMRRANSTEPAALLGALATTRYDGVIGRIRFSPQGDNLNGAVTVY
EEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEE
RVQRGALVPVSD
EEECCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA