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The map label for this gene is purU [H]

Identifier: 126452460

GI number: 126452460

Start: 574899

End: 575780

Strand: Direct

Name: purU [H]

Synonym: BURPS1106A_0607

Alternate gene names: 126452460

Gene position: 574899-575780 (Clockwise)

Preceding gene: 126451590

Following gene: 126452737

Centisome position: 14.41

GC content: 67.01

Gene sequence:

>882_bases
ATGCCGGCCGATCACAGCTTTATCCTGAAACTGTCGTGCCCGGACCGGCGCGGCATCGTTCACGCGGTCTCGGGCTTTCT
GCTCGAGCGCGGCAGCAATATCCTCGATTCGGCGCAGTTCGGCGACAGCCGCACCGGCGAATTCTTCATGCGCGTGCATT
TCGAGCAGGCGGGCGCGTGCGGCGCGGCGGCCGCGCTCGACGCCCTGCGCGCCGAGTTCGCGCCGCTCGCCGAGGCGTTC
GCGATGCGCTGGGAGCTGCACGACGCGGCGGTGAAGCCGCGCGTCGTGATCCTCGTGTCGAAGATCGGCCATTGCCTGAA
CGACTTGCTGTTCCGCTACCGCACGGGCCAGTTGCCGATCGAGATCTCGGCGATCGTGTCGAACCACAAGGATTTCTATC
AGCTCGCCGCGAGTTACGACATTCCGTTCCATCATCTCCCGCTCGCCGCGGGCGCGTCGGCCGACGCGAAAGCCGCGCAG
GAAGCGCGCGTGCTCGAGGTGATCGACGGGCACGCGGCCGATCTCGTCGTGCTCGCGCGCTACATGCAGATCCTGTCGCC
CGCCCTGTGCGAGCGGCTCGCGGGGCGCGCGATCAACATCCATCACTCGTTCTTGCCGAGCTTCAAGGGCGCGAAGCCGT
ACTACCAGGCGTTCGACCGCGGCGTGAAGCTGATCGGCGCGACCGCGCACTACGTGACGACCGACCTCGACGAAGGCCCG
ATCATCGAGCAGGAAGTCGAGCGCGTCGATCACAGCATGACGCCGGACGAGCTGACGGCCGTCGGCCGCGACGTCGAATG
CGTGACGCTCGCGCGCGCGGTCAAGTGGCACGTCGAGCACCGGATCGTGCTGAACGGCACGAAGACGGTGGTGTTCCGCT
AA

Upstream 100 bases:

>100_bases
GCGCGGAATCGACGTGCTGTTCGCGCCGCCGCCGCCGGGCCTGGGTTGAGCGCGGCGCATTGTTCGGAAGATTTGAGAAT
TTGAGAAGGGAGTCGCGGTC

Downstream 100 bases:

>100_bases
GCCGGCGCGCGCCCCGCCTTTCACGGGCCGGCACGCCGCGCGCGGCAGGCGGGCGCGGGCTGCCGGCGGCGCGTGCCGCC
GCGCGGGGACGAAAAAAGGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MPADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGACGAAAALDALRAEFAPLAEAF
AMRWELHDAAVKPRVVILVSKIGHCLNDLLFRYRTGQLPIEISAIVSNHKDFYQLAASYDIPFHHLPLAAGASADAKAAQ
EARVLEVIDGHAADLVVLARYMQILSPALCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR

Sequences:

>Translated_293_residues
MPADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGACGAAAALDALRAEFAPLAEAF
AMRWELHDAAVKPRVVILVSKIGHCLNDLLFRYRTGQLPIEISAIVSNHKDFYQLAASYDIPFHHLPLAAGASADAKAAQ
EARVLEVIDGHAADLVVLARYMQILSPALCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
>Mature_292_residues
PADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGACGAAAALDALRAEFAPLAEAFA
MRWELHDAAVKPRVVILVSKIGHCLNDLLFRYRTGQLPIEISAIVSNHKDFYQLAASYDIPFHHLPLAAGASADAKAAQE
ARVLEVIDGHAADLVVLARYMQILSPALCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGPI
IEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=175, Percent_Identity=33.1428571428571, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI209869995, Length=175, Percent_Identity=33.1428571428571, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI209869993, Length=175, Percent_Identity=33.1428571428571, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1787483, Length=285, Percent_Identity=39.6491228070175, Blast_Score=214, Evalue=6e-57,
Organism=Escherichia coli, GI1788846, Length=194, Percent_Identity=31.4432989690722, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=33.6898395721925, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32245; Mature: 32114

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGAC
CCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEEHHCCCC
GAAAALDALRAEFAPLAEAFAMRWELHDAAVKPRVVILVSKIGHCLNDLLFRYRTGQLPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCE
EISAIVSNHKDFYQLAASYDIPFHHLPLAAGASADAKAAQEARVLEVIDGHAADLVVLAR
EEHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
YMQILSPALCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
HHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCC
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
CCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEC
>Mature Secondary Structure 
PADHSFILKLSCPDRRGIVHAVSGFLLERGSNILDSAQFGDSRTGEFFMRVHFEQAGAC
CCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEEEHHCCCC
GAAAALDALRAEFAPLAEAFAMRWELHDAAVKPRVVILVSKIGHCLNDLLFRYRTGQLPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCE
EISAIVSNHKDFYQLAASYDIPFHHLPLAAGASADAKAAQEARVLEVIDGHAADLVVLAR
EEHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
YMQILSPALCERLAGRAINIHHSFLPSFKGAKPYYQAFDRGVKLIGATAHYVTTDLDEGP
HHHHHCHHHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEEECCCCCC
IIEQEVERVDHSMTPDELTAVGRDVECVTLARAVKWHVEHRIVLNGTKTVVFR
CCHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]