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The map label for this gene is iscS [H]

Identifier: 126452227

GI number: 126452227

Start: 2613754

End: 2614977

Strand: Reverse

Name: iscS [H]

Synonym: BURPS1106A_2655

Alternate gene names: 126452227

Gene position: 2614977-2613754 (Counterclockwise)

Preceding gene: 126453431

Following gene: 126454599

Centisome position: 65.56

GC content: 64.79

Gene sequence:

>1224_bases
ATGAACAACGATATCCCCCACCTGCCCATCTACATGGACTACAGCGCGACGACCCCCGTCGATCCGCGCGTCGTCGACAA
GATGGTGCCGTATCTGCGCGAGCAGTTCGGCAACCCGGCGTCGCGCAGCCACGCATACGGCTGGGACGCGGAGCGCGCGG
TCGAAGAGGCGCGCGAGCAGGTGGCCGCCCTCGTGAACGCCGATCCGCGCGAGATCATCTGGACCTCCGGCGCGACCGAG
TCCGACAACCTCGCGATCAAGGGCGCCGCGCACTTCTATCAGGGCAAGGGCAAGCACATCGTCACCGTGAAGACCGAGCA
CAAGGCGGTGCTCGACACCTGCCGCGAGCTCGAGCGCGAAGGCTTCGAGGTGACCTATCTCGACGTGAAGGACGACGGGC
TCGTCGATCTCGACGTGTTCAAGGCCGCGCTGCGCCCGGACACGATCCTCGTGTCGGTGATGCATGTGAACAACGAGATC
GGCGTGATCCAGGACATCGCGACGATCGGCGAGATCTGCCGCGAGAAGGGCATCATCTTCCACGTCGACGCCGCGCAGGC
GACGGGCAAGGTCGAAATCGACCTCGCGAAGCTGAAGGTCGACCTGATGTCGTTCTCCGCGCACAAGACCTACGGCCCGA
AGGGCATCGGCGCGTTGTATGTGCGCCGCAAGCCGCGCGTGCGCATCGAGGCGCAGATGCACGGCGGCGGCCACGAGCGC
GGCATGCGCTCGGGCACGTTGCCGACGCACCAGATCGTCGGCATGGGCGAGGCGTTTCGCATCGCGCGCGAAGAGATGGC
GACCGAGAACGAGCGCATCCGGATGCTGCGCGACAAGCTGCTGCGCGGCCTGTCGGAAATCGACGAAACCTACGTGAACG
GCGATCTCGAGCACCGGATTCCGCACAACCTGAACATCAGCTTCAATTTTGTCGAAGGCGAATCACTGATCATGGCGATC
AAGGACGTCGCGGTGTCGTCGGGCTCCGCGTGCACGTCGGCGTCGCTCGAGCCGTCCTACGTGCTGCGCGCGCTCGGCCG
CAACGACGAGCTCGCGCACAGCTCGATCCGCTTCACGGTCGGCCGCTTCACGACGGAGCAGGAAGTCGACTACGTGATCG
ACCTGCTGAAGAGCAAGATCGCGAAGCTGCGCGACCTGTCGCCGCTTTGGGAGATGCATCAGGAAGGCATCGATCTGTCG
ACGATCGAATGGGCGGCGCACTGA

Upstream 100 bases:

>100_bases
CGAATTCGGTCTTCAACATCGCGAATTCATGATGGCCGCGCACTGAACGCGGCCCGTACAACGAATAGTCCCCGCACTGA
ATGCATACCCGGAGCGATAG

Downstream 100 bases:

>100_bases
GCGCGGCGACTTTTTCGCGGGCGCCGGCGCGCCGCGCAGACGAAACGAAGCAAGGAGTTTGAGTCATGTCTTACAGCAAC
AAGGTTCTGGACCACTACGA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MNNDIPHLPIYMDYSATTPVDPRVVDKMVPYLREQFGNPASRSHAYGWDAERAVEEAREQVAALVNADPREIIWTSGATE
SDNLAIKGAAHFYQGKGKHIVTVKTEHKAVLDTCRELEREGFEVTYLDVKDDGLVDLDVFKAALRPDTILVSVMHVNNEI
GVIQDIATIGEICREKGIIFHVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHER
GMRSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDKLLRGLSEIDETYVNGDLEHRIPHNLNISFNFVEGESLIMAI
KDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYVIDLLKSKIAKLRDLSPLWEMHQEGIDLS
TIEWAAH

Sequences:

>Translated_407_residues
MNNDIPHLPIYMDYSATTPVDPRVVDKMVPYLREQFGNPASRSHAYGWDAERAVEEAREQVAALVNADPREIIWTSGATE
SDNLAIKGAAHFYQGKGKHIVTVKTEHKAVLDTCRELEREGFEVTYLDVKDDGLVDLDVFKAALRPDTILVSVMHVNNEI
GVIQDIATIGEICREKGIIFHVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHER
GMRSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDKLLRGLSEIDETYVNGDLEHRIPHNLNISFNFVEGESLIMAI
KDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYVIDLLKSKIAKLRDLSPLWEMHQEGIDLS
TIEWAAH
>Mature_407_residues
MNNDIPHLPIYMDYSATTPVDPRVVDKMVPYLREQFGNPASRSHAYGWDAERAVEEAREQVAALVNADPREIIWTSGATE
SDNLAIKGAAHFYQGKGKHIVTVKTEHKAVLDTCRELEREGFEVTYLDVKDDGLVDLDVFKAALRPDTILVSVMHVNNEI
GVIQDIATIGEICREKGIIFHVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHER
GMRSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDKLLRGLSEIDETYVNGDLEHRIPHNLNISFNFVEGESLIMAI
KDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYVIDLLKSKIAKLRDLSPLWEMHQEGIDLS
TIEWAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=59.75, Blast_Score=527, Evalue=1e-150,
Organism=Homo sapiens, GI156713448, Length=418, Percent_Identity=33.0143540669856, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI48994898, Length=402, Percent_Identity=74.6268656716418, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=229, Percent_Identity=29.2576419213974, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1787970, Length=225, Percent_Identity=27.1111111111111, Blast_Score=92, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=61.3466334164589, Blast_Score=517, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=32.7217125382263, Blast_Score=145, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=58.1453634085213, Blast_Score=507, Evalue=1e-144,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=58, Blast_Score=492, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45455; Mature: 45455

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNDIPHLPIYMDYSATTPVDPRVVDKMVPYLREQFGNPASRSHAYGWDAERAVEEAREQ
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VAALVNADPREIIWTSGATESDNLAIKGAAHFYQGKGKHIVTVKTEHKAVLDTCRELERE
HHHHHCCCCHHEEEECCCCCCCCEEEEECHHHCCCCCCEEEEEECCHHHHHHHHHHHHHC
GFEVTYLDVKDDGLVDLDVFKAALRPDTILVSVMHVNNEIGVIQDIATIGEICREKGIIF
CCEEEEEEECCCCEEEHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
HVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHER
EEECCCCCCEEEEEHHHEEEEHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHC
GMRSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDKLLRGLSEIDETYVNGDLEHRI
CCCCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
PHNLNISFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTV
CCCCCEEEEEECCCEEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCEEEEEE
GRFTTEQEVDYVIDLLKSKIAKLRDLSPLWEMHQEGIDLSTIEWAAH
ECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure
MNNDIPHLPIYMDYSATTPVDPRVVDKMVPYLREQFGNPASRSHAYGWDAERAVEEAREQ
CCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
VAALVNADPREIIWTSGATESDNLAIKGAAHFYQGKGKHIVTVKTEHKAVLDTCRELERE
HHHHHCCCCHHEEEECCCCCCCCEEEEECHHHCCCCCCEEEEEECCHHHHHHHHHHHHHC
GFEVTYLDVKDDGLVDLDVFKAALRPDTILVSVMHVNNEIGVIQDIATIGEICREKGIIF
CCEEEEEEECCCCEEEHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEE
HVDAAQATGKVEIDLAKLKVDLMSFSAHKTYGPKGIGALYVRRKPRVRIEAQMHGGGHER
EEECCCCCCEEEEEHHHEEEEHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHC
GMRSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDKLLRGLSEIDETYVNGDLEHRI
CCCCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCC
PHNLNISFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTV
CCCCCEEEEEECCCEEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCEEEEEE
GRFTTEQEVDYVIDLLKSKIAKLRDLSPLWEMHQEGIDLSTIEWAAH
ECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA